Chitosan-based electrospun nanofibrous mats, hydrogels and cast films: novel anti-bacterial wound dressing matrices

  • Sohail Shahzad
  • Muhammad Yar
  • Saadat Anwar Siddiqi
  • Nasir Mahmood
  • Abdul Rauf
  • Zafar-ul-Ahsan Qureshi
  • Muhammad Sabieh Anwar
  • Shahida Afzaal
Delivery Systems
Part of the following topical collections:
  1. Delivery Systems

Abstract

The development of highly efficient anti-bacterial wound dressings was carried out. For this purpose nanofibrous mats, hydrogels and films were synthesized from chitosan, poly(vinyl alcohol) and hydroxyapatite. The physical/chemical interactions of the synthesized materials were evaluated by FTIR. The morphology, structure; average diameter and pore size of the materials were investigated by scanning electron microscopy. The hydrogels showed a greater degree of swelling as compared to nanofibrous mats and films in phosphate buffer saline solution of pH 7.4. The in vitro drug release studies showed a burst release during the initial period of 4 h and then a sustained release profile was observed in the next upcoming 20 h. The lyophilized hydrogels showed a more slow release as compared to nanofibrous mats and films. Antibacterial potential of drug released solutions collected after 24 h of time interval was determined and all composite matrices showed good to moderate activity against Gram-positive and Gram-negative bacterial strains respectively. To determine the cytotoxicity, cell culture was performed for various cefixime loaded substrates by using neutral red dye uptake assay and all the matrices were found to be non-toxic.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Sohail Shahzad
    • 1
  • Muhammad Yar
    • 2
  • Saadat Anwar Siddiqi
    • 2
  • Nasir Mahmood
    • 3
    • 4
  • Abdul Rauf
    • 1
  • Zafar-ul-Ahsan Qureshi
    • 5
  • Muhammad Sabieh Anwar
    • 6
  • Shahida Afzaal
    • 5
  1. 1.Department of ChemistryThe Islamia University of BahawalpurBahawalpurPakistan
  2. 2.Interdisciplinary Research Center in Biomedical MaterialsCOMSATS Institute of Information TechnologyLahorePakistan
  3. 3.Department of Allied Health Sciences and Chemical PathologyUniversity of Health SciencesLahorePakistan
  4. 4.Department of Human Genetics and Molecular BiologyUniversity of Health SciencesLahorePakistan
  5. 5.Veterinary Research InstituteLahorePakistan
  6. 6.Department of Physics, Syed Babar Ali School of Science and EngineeringLahore University of Management Sciences (LUMS)LahorePakistan

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