Ectopic osteogenesis of an injectable nHAC/CSH loaded with blood-acquired mesenchymal progenitor cells in a nude mice model
- 209 Downloads
An injectable bone cement, nHAC/CSH, which consists of nano-hydroxyapatite/collagen (nHAC) and calcium sulphate hemihydrate (CaSO4.½H2O; CSH) was investigated as a tissue-engineered scaffold material with blood-acquired mesenchymal progenitor cells (BMPCs) as seeding cells. An in vitro study on the cytocompatability of nHAC/CSH and an in vivo study on the ectopic bone formation of nHAC/CSH loaded with dBMPCs were both conducted. The dBMPCs morphology, proliferation, differentiation and apoptosis assays were conducted using the direct contact and extract method. The cells tests exhibited normal growth and bioactive function in vitro. Studies in vivo showed that this injectable tissue engineered bone (ITB) formed bone structure in the heterotopic site of nude mice. These findings indicate that the ITB composed of nHAC/CSH and dBMPCs may represent a useful strategy for clinical reconstruction of irregular bone defects.
KeywordsOsteogenic Differentiation Extract Medium Nude Mouse Model Ectopic Bone Formation Injectable Cement
This work is supported by Postdoctoral Science Foundation of China No.20100481483 and National Natural Science Foundation of China No.81271180. The authors report no conflicts of interest related to this study.
- 8.Pecora GE, De Leonardis D, Della Rocca C, Cornelini R, Cortesini C. Short-term healing following the use of calcium sulfate as a grafting material for sinus augmentation: a clinical report. Int J Oral Maxillo-fac Implants. 1998;13:866–73.Google Scholar
- 13.Liu X, Wang XM, Chen Z, Cui FZ, Liu HY, Mao K, Wang Y. Injectable bone cement based on mineralized collagen. J Biomed Mater Res B Appl Biomater. 2010;94:72–9.Google Scholar
- 16.Cesselli D, Beltrami AP, Rigo S, Bergamin N, D’Aurizio F, Verardo R, Piazza S, Klaric E, Fanin R, Toffoletto B, Marzinotto S, Mariuzzi L, Finato N, Pandolfi M, Leri A, Schneider C, Beltrami CA, Anversa P. Multipotent progenitor cells are present in human peripheral blood. Circ Res. 2009;104:1225–34.CrossRefGoogle Scholar
- 20.Han X, Liu H, Wang D, Su F, Zhang Y, Zhou W, Li S, Yang R. Alveolar bone regeneration around immediate implants using an injectable nHAC/CSH loaded with autogenic blood-acquired mesenchymal progenitor cells: an experimental study in the dog mandible. Clin Implant Dent Relat Res. 2013;15:390–401.CrossRefGoogle Scholar
- 24.Guarnieri R, Bovi M. Maxillary sinus augmentation using prehardened calcium sulfate: a case report. Int J Periodontics Restorative Dent. 2002;22:503–8.Google Scholar
- 27.Nilsson M, Wang JS, Wielanek L, Tanner KE, Lidgren L. Biodegradation and biocompatability of a calcium sulphate-hydroxyapatite bone substitute. J Bone Joint Surg Br. 2004;86:120–5.Google Scholar
- 31.Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol. 1990;143:420–30.CrossRefGoogle Scholar