Journal of Materials Science: Materials in Medicine

, Volume 25, Issue 12, pp 2711–2720 | Cite as

Evaluation of new bone formation in irradiated areas using association of mesenchymal stem cells and total fresh bone marrow mixed with calcium phosphate scaffold

  • P. BléryEmail author
  • P. Corre
  • O. Malard
  • S. Sourice
  • P. Pilet
  • Y. Amouriq
  • J. Guicheux
  • P. Weiss
  • F. Espitalier


The consequences of the treatment of the squamous cell carcinomas of the upper aerodigestive tract (bone removal and external radiation therapy) are constant. Tissue engineering using biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSC) is considered as a promising alternative. We previously demonstrated the efficacy of BCP and total fresh bone marrow (TBM) in regenerating irradiated bone defect. The aim of this study was to know if adding MSC to BCP + TBM mixture could improve the bone formation in irradiated bone defects. Twenty-four Lewis 1A rats received a single dose of 20 Gy to the hind limbs. MSC were sampled from non-irradiated donors and amplified in proliferative, and a part in osteogenic, medium. 3 weeks after, defects were created on femurs and tibias, which were filled with BCP alone, BCP + TBM, BCP + TBM + uncommitted MSC, or BCP + TBM + committed MSC. 3 weeks after, samples were removed and prepared for qualitative and quantitative analysis. The rate of bone ingrowth was significantly higher after implantation of BCP + TBM mixture. The adding of a high concentration of MSC, committed or not, didn’t improve the bone regeneration. The association BCP + TBM remains the most efficient material for bone substitution in irradiated areas.


Bone Formation Mesenchymal Stem Cell Bone Defect Osteogenic Differentiation Bone Ingrowth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by Grants from “La Ligue Contre Le Cancer” foundation, committees “Pays de La Loire” and “Côtes d’Armor”. We thank Biomatlante (Vigneux de Bretagne, France) for supplying materials. We thank Doctor Marion Eveillard for the bone marrow myelographic analysis.

Conflict of interest

The authors declare no industrial conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • P. Bléry
    • 1
    • 2
    • 3
    • 4
    • 5
    Email author
  • P. Corre
    • 1
    • 2
    • 4
    • 6
  • O. Malard
    • 1
    • 2
    • 4
    • 7
  • S. Sourice
    • 1
    • 2
  • P. Pilet
    • 1
    • 2
    • 4
  • Y. Amouriq
    • 1
    • 2
    • 3
    • 4
    • 5
  • J. Guicheux
    • 1
    • 2
    • 4
  • P. Weiss
    • 1
    • 2
    • 3
    • 4
  • F. Espitalier
    • 1
    • 2
    • 4
    • 7
  1. 1.INSERM, UMR-S 791, Laboratoire d’Ingénierie Ostéo-Articulaire et Dentaire, LIOADNantes Cedex 1France
  2. 2.Université de Nantes, Laboratoire d’Ingénierie Ostéo-Articulaire et Dentaire, LIOADNantes Cedex 1France
  3. 3.Université de Nantes, Faculté de Chirurgie DentaireNantes Cedex 1France
  4. 4.CHU Nantes, PHU 4 OTONNNantes Cedex 1France
  5. 5.CHU Nantes, PHU 4 OTONN, Service d’Odontologie Restauratrice et ChirurgicaleNantes Cedex 1France
  6. 6.CHU Nantes, PHU 4 OTONN, Service de Stomatologie et de Chirurgie Maxillo-FacialeNantes Cedex 1France
  7. 7.CHU Nantes, PHU 4 OTONN, Service d’ORL et de Chirurgie Cervico-FacialeNantes Cedex 1France

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