Journal of Materials Science: Materials in Medicine

, Volume 25, Issue 12, pp 2669–2676 | Cite as

Multilayered implantation using acellular dermal matrix into nude mice

  • Dong Won Lee
  • Myung Chul Lee
  • Hyun Roh
  • Won Jai LeeEmail author


Soft tissue augmentation using acellular dermal matrix has gained popularity to overcome the shortcomings of autogenous and alloplastic materials. Sometimes it needs multilayered stacking to obtain enough volume. In this study, we investigated the efficacy of multilayered implantation using acellular dermal matrix (MatriDerm®) for soft tissue augmentation. MatriDerm was implanted subdermally on each side of the dorsum of nude mice (n = 20), stacked two layers thick in the control group and three layers thick in the experimental group. Alterations of thickness, degree of angiogenesis, and collagen and elastin fiber syntheses were observed over 40 days. Three-layered implantation with MatriDerm maintained its volume similarly as in two-layered implantation, although the thickness decreased after 30 days in both groups. At the early stage of implantation, angiogenesis and collagen and elastin fiber syntheses occurred fluently on the central portion, which is the farthest away from the surface in contact with the host tissue. Collagen and elastin fibers became more concentrated over time, and the original structure of MatriDerm could not be maintained due to being replaced with newly formed collagen and elastin fibers 40 days after implantation. Multilayered implantation with MatriDerm is considered appropriate for tissue ingrowth and can be used as a substitute for soft tissue augmentation.


Middle Layer Acellular Dermal Matrix Elastin Fiber Acid Fuchsin Alloplastic Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (No. 2011-0022012, Lee WJ and No. 2013R1A1A1009764, Lee DW).


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Dong Won Lee
    • 1
  • Myung Chul Lee
    • 1
  • Hyun Roh
    • 1
  • Won Jai Lee
    • 1
    Email author
  1. 1.Department of Plastic and Reconstructive Surgery, Institute for Human Tissue RestorationYonsei University College of MedicineSeoulKorea

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