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Effects of the polymeric niche on neural stem cell characteristics during primary culturing

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Abstract

The polymeric niche encountered by cells during primary culturing can affect cell fate. However, most cell types are primarily propagated on polystyrene (PS). A cell type specific screening for optimal primary culture polymers particularly for regenerative approaches seems inevitable. The effect of physical and chemical properties of treated (corona, oxygen/nitrogen plasma) and untreated cyclic olefin polymer (COP), polymethymethacrylate (PMMA), PP, PLA, PS, PC on neuronal stem cell characteristics was analyzed. Our comprehensive approach revealed plasma treated COP and PMMA as optimal polymers for primary neuronal stem cell culturing and propagation. An increase in the number of NT2/D1 cells with pronounced adhesion, metabolic activities and augmented expression of neural precursor markers was associated to the plasma treatment of surfaces of COP and PMMA with nitrogen or oxygen, respectively. A shift towards large cell sizes at stable surface area/volume ratios that might promote the observed increase in metabolic activities and distinct modulations in F-actin arrangements seem to be primarily mediated by the plasma treatment of surfaces. These results indicate that the polymeric niche has a distinct impact on various cell characteristics. The selection of distinct polymers and the controlled design of an optimized polymer microenvironment might thereby be an effective tool to promote essential cell characteristics for subsequent approaches.

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Abbreviations

COP:

Cyclic olefin polymer

PMMA:

Polymethymethacrylate

PP:

Polypropylene

PLA:

Polylactic acid

PS:

Polystyrene

PC:

Polycarbonate

Polymeric niche:

Chemical and physical polymer surface characteristics encountered by cultured cells

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Acknowledgments

We thank Paul Freudenberger for his support and helpful comments. This work was supported by a FFG grant (Project Number 824915).

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Authors and Affiliations

  1. Research Unit for Experimental Neurotraumatology, Department of Neurosurgery, Medical University of Graz, Auenbruggerplatz 2/2, 8036, Graz, Austria

    Stefan Haubenwallner, Ulrike Fasching, Silke Patz, Christa Trattnig, Natascha Andraschek, Gerda Grünbacher & Ute Schäfer

  2. Department of Polymer Engineering and Science, Montanuniversitaet Leoben, Franz Josef Strasse 18, 8700, Leoben, Austria

    Matthias Katschnig, Stephan Laske & Clemens Holzer

  3. Core Facility Microscopy, Centre of Medical Research, Medical University of Graz, Stiftingtalstrasse 24, 8010, Graz, Austria

    Markus Absenger

  4. Sony DADC Austria AG BioSciences, Sonystrasse 20, 5081, Anif, Salzburg, Austria

    Werner Balika & Manuela Wagner

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  1. Stefan Haubenwallner
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Correspondence to Ute Schäfer.

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Stefan Haubenwallner and Matthias Katschnig have contributed equally to this work.

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Fig. S1

Mean values (n = 6) obtained after 2 h to create a standard curve starting with 2 × 103–3.2 × 106 cells for the generation of a linear equation y = mx + b.; n = 4. Supplementary material 1 (PPT 108 kb)

Fig. S2

AFM 3-D surface images of treated and untreated polymers. Supplementary material 2 (PPT 843 kb)

Fig. S3

Time dependent hydrophobic recovery of treated polymers a Time dependent changes in contact angles of corona treated polymers b Time dependent changes in contact angles of plasma treated polymers; n = 12 for each data set. Supplementary material 3 (PPT 134 kb)

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Haubenwallner, S., Katschnig, M., Fasching, U. et al. Effects of the polymeric niche on neural stem cell characteristics during primary culturing. J Mater Sci: Mater Med 25, 1339–1355 (2014). https://doi.org/10.1007/s10856-014-5155-y

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