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Highly sensitive pyrogen detection on medical devices by the monocyte activation test

  • Katharina Stang
  • Stefan Fennrich
  • Stefanie Krajewski
  • Sandra Stoppelkamp
  • Iwan Anton Burgener
  • Hans-Peter WendelEmail author
  • Marcell Post
Article

Abstract

Pyrogens are components of microorganisms, like bacteria, viruses or fungi, which can induce a complex inflammatory response in the human body. Pyrogen contamination on medical devices prior operation is still critical and associated with severe complications for the patients. The aim of our study was to develop a reliable test, which allows detection of pyrogen contamination on the surface of medical devices. After in vitro pyrogen contamination of different medical devices and incubation in a rotation model, the human whole blood monocyte activation test (MAT), which is based on an IL-1β-specific ELISA, was employed. Our results show that when combining a modified MAT protocol and a dynamic incubation system, even smallest amounts of pyrogens can be directly detected on the surface of medical devices. Therefore, screening of medical devices prior clinical application using our novel assay, has the potential to significantly reduce complications associated with pyrogen-contaminated medical devices.

Keywords

Medical Device Systemic Inflammatory Response Syndrome Stainless Steel Plate Stainless Steel Sample ePTFE Graft 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors would like to express their gratitude to the companies Qualimed and Jotec, who generously made the stents and vascular grafts available.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Katharina Stang
    • 1
  • Stefan Fennrich
    • 1
  • Stefanie Krajewski
    • 1
  • Sandra Stoppelkamp
    • 1
  • Iwan Anton Burgener
    • 2
  • Hans-Peter Wendel
    • 1
    Email author
  • Marcell Post
    • 1
  1. 1.Clinical Research Laboratory, Department of Thoracic, Cardiac and Vascular SurgeryUniversity Hospital Tuebingen, Tuebingen UniversityTuebingenGermany
  2. 2.Department of Small Animal Medicine, Faculty of Veterinary MedicineUniversity of LeipzigLeipzigGermany

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