Journal of Materials Science: Materials in Medicine

, Volume 24, Issue 11, pp 2491–2503 | Cite as

Recent advances in synthesis, characterization of hydroxyapatite/polyurethane composites and study of their biocompatible properties

  • L. M. Popescu
  • R. M. Piticescu
  • A. Antonelli
  • C. F. Rusti
  • E. Carboni
  • C. Sfara
  • M. Magnani
  • V. Badilita
  • E. Vasile
  • R. Trusca
  • T. Buruiana
Article
First Online:
Received:
Accepted:

Abstract

The development of engineered biomaterials that mimic bone tissues is a promising research area that benefits from a growing interest. Polymers and polymer–ceramic composites are the principle materials investigated for the development of synthetic bone scaffolds thanks to their proven biocompatibility and biostability. Several polymers have been combined with calcium phosphates (mainly hydroxyapatite) to prepare nanocomposites with improved biocompatible and mechanical properties. Here, we report the hydrothermal synthesis in high pressure conditions of nanostructured composites based on hydroxyapatite and polyurethane functionalized with carboxyl and thiol groups. Cell-material interactions were investigated for potential applications of these new types of composites as coating for orthopedic implants. Physical–chemical and morphological characteristics of hydroxyapatite/polyurethane composites were evaluated for different compositions, showing their dependence on synthesis parameters (pressure, temperature). In vitro experiments, performed to verify if these composites are biocompatible cell culture substrates, showed that they are not toxic and do not affect cell viability.

Keywords

HeLa Cell Polyurethane Hydroxyapatite High Resolution Transmission Electron Microscope High Resolution Transmission Electron Microscope 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

L. M. P. and T. B. would like to acknowledge the financial support of European Social Fund—“Cristofor I. Simionescu” Postdoctoral Fellowship Programme (ID POSDRU/89/1.5/S/55216), Sectoral Operational Programme Human Resources Development 2007–2013. R.M.P would like to acknowledge COST TD0802 Programme. The authors would like to thank Dr. Michele Menotta for AFM analyses. L. M. P and R. M. P have equal contributions to this paper.

References

  1. 1.
    Swetha M, Sahithi K, Moorthi A, Srinivasan N, Ramasamy K, Selvamurugan N. Biocomposites containing natural polymers and hydroxyapatite for bone tissue engineering. Int J Biol Macromol. 2010;47:1–4. doi: 10.1016/j.ijbiomac.2010.03.015.CrossRefGoogle Scholar
  2. 2.
    Sun F, Zhou H, Lee J. Various preparation methods of highly porous hydroxyapatite/polymer nanoscale biocomposites for bone regeneration. Acta Biomater. 2011;7:3813–28. doi: 10.1016/j.actbio.2011.07.002.CrossRefGoogle Scholar
  3. 3.
    Mourino V, Boccaccini AR. Bone tissue engineering therapeutics:controlled drug delivery in three-dimensional scaffolds. J R Soc Interface. 2010;7:209–27. doi: 10.1098/rsif.2009.0379.CrossRefGoogle Scholar
  4. 4.
    Porter JR, Ruckh TT, Popat KC. Bone tissue engineering: a review in bone biomimetics and drug delivery strategies. Biotechnol Prog. 2009;25:1539–60. doi: 10.1002/btpr.246.Google Scholar
  5. 5.
    Neuendorf RE, Saiz E, Tomsia AP, Ritchie RO. Adhesion between biodegradable polymers and hydroxyapatite: relevance to synthetic bone-like materials and tissue engineering scaffolds. Acta Biomater. 2008;4:1288–96. doi: 10.1016/j.actbio.2008.04.006.CrossRefGoogle Scholar
  6. 6.
    Supova M. Problem of hydroxyapatite dispersion in polymer matrices: a review. J Mater Sci Mater Med. 2009;20:1201–1213; and references herein. doi: 10.1007/s10856-009-3696-2.Google Scholar
  7. 7.
    Bertoldi S, Farè S, Ciapetti G, Tanzi MC. Polyurethane foams and Ca–P composites for bone tissue engineering. J Appl Biomater Biomech. 2007;5(3):195.Google Scholar
  8. 8.
    Armentano I, Dottori M, Fortunati E, Mattioli S, Kenny JM. Biodegradable polymer matrix nanocomposites for tissue engineering: a review. Polym Degrad Stab. 2010;95:2126–46. doi: 10.1016/j.polymdegradstab.2010.06.007.CrossRefGoogle Scholar
  9. 9.
    Habraken WJEM, Wolke JGC, Jansen JA. Ceramic composites as matrices and scaffolds for drug delivery in tissue engineering. Adv Drug Deliv Rev. 2007;59:234–48. doi: 10.1016/j.addr.2007.03.011.CrossRefGoogle Scholar
  10. 10.
    Ma PX. Biomimetic materials for tissue engineering. Adv Drug Deliv Rev. 2008;60:184–98. doi: 10.1016/j.addr.2007.08.041.CrossRefGoogle Scholar
  11. 11.
    Laurencin CT, Attawia MA, Elgendy HE, Herbert KM. Tissue engineered bone-regeneration using degradable polymers: the formation of mineralized matrices. Bone. 1996;19:93S–9S.CrossRefGoogle Scholar
  12. 12.
    Deplaine H, Gómez Ribelles JL, Gallego Ferrer G. Effect of the content of hydroxyapatite nanoparticles on the properties and bioactivity of poly(l-lactide)—hybrid membranes. Compos Sci Technol. 2010;70:1805–12. doi: 10.1016/j.compscitech.2010.06.009.CrossRefGoogle Scholar
  13. 13.
    Lee JE, Park JC, Kwang HL, Sang HO, Kim JG, Hwal S. An infection-preventing bilayered collagen membrane containing antibiotic-loaded hyaluronan microparticles: physical and biological properties. Artif Organs. 2002;26:636–46.CrossRefGoogle Scholar
  14. 14.
    Park SN, Kim JK, Suh H. Evaluation of antibiotic-loaded collagen-hyaluronic acid matrix as a skin substitute. Biomaterials. 2004;25:3689–98. doi: 10.1016/j.biomaterials.2003.10.072.CrossRefGoogle Scholar
  15. 15.
    Sripriya R, Kumar MS, Sehgal PK. Improved collagen bilayer dressing for the controlled release of drugs. J Biomed Mater Res B Appl Biomater. 2004;70:389–96.CrossRefGoogle Scholar
  16. 16.
    Prabu P, Dharmaraj N, Aryal S, Lee BM, Ramesh V, Kim HY. Preparation and drug release activity of scaffolds containing collagen and poly(caprolactone). J Biomed Mater Res A. 2006;79:153–8. doi: 10.1002/jbm.a.30715.Google Scholar
  17. 17.
    Shanmugasundaram N, Sundaraseelan J, Uma S, Selvaraj D, Babu M. Design and delivery of silver sulfadiazine from alginate microspheres-impregnated collagen scaffold. J Biomed Mater Res B Appl Biomater. 2006;77:378–88. doi: 10.1002/jbm.b.30405.Google Scholar
  18. 18.
    Muzzarelli R, Tarsi R, Filippini O, Giovanetti E, Biagini G, Varaldo PE. Antimicrobial properties of N-carboxybutyl chitosan. Antimicrob Agents Chemother. 1990;34:2019–23. doi: 10.1128/AAC.34.10.2019.CrossRefGoogle Scholar
  19. 19.
    Chung LY, Schmidt RJ, Hamlyn PF, Sagar BF, Andrews AM, Turner TD. Biocompatibility of potential wound management products: fungal mycelia as a source of chitin/chitosan and their effect on the proliferation of human F1000 fibroblasts in culture. J Biomed Mater Res. 1994;24:463–9.CrossRefGoogle Scholar
  20. 20.
    Mi F-L, Wu Y-B, Shyu S–S, Schoung J-Y, Huang Y-B, Tsai Y-H, Hao J-Y. Control of wound infections using a bilayer chitosan wound dressing with sustainable antibiotic delivery. J Biomed Mater Res. 2002;59:438–49. doi: 10.1002/jbm.1260.CrossRefGoogle Scholar
  21. 21.
    Aoyagi S, Onishi H, Machida Y. Novel chitosan wound dressing loaded with minocycline for the treatment of severe burn wounds. Int J Pharm. 2007;330:138–45. doi: 10.1016/j.ijpharm.2006.09.016.CrossRefGoogle Scholar
  22. 22.
    Rossi S, Marciello M, Sandri G, Ferrari F, Bonferoni MC, Papetti A, Caramella C, Dacarro C, Grisoli P. Wound dressings based on chitosans and hyaluronic acid for the release of chlorhexidine diacetate in skin ulcer therapy. Pharm Dev Technol. 2007;12:415–22. doi: 10.1080/10837450701366903.CrossRefGoogle Scholar
  23. 23.
    Munarin F, Petrini P, Tanzi MC, Barbosa MA, Granja PL. Biofunctional chemically modified pectin for cell delivery. Soft Matter. 2012;8:4731–9. doi: 10.1039/C2SM07260B.CrossRefGoogle Scholar
  24. 24.
    Munarin F, Guerreiro SG, Grellier MA, Tanzi MC, Barbosa MA, Petrini P, Granja PL. Pectin-based injectable biomaterials for bone tissue engineering. Biomacromolecules. 2011;12:568–77. doi: 10.1021/bm101110x.CrossRefGoogle Scholar
  25. 25.
    Popescu LM, Rusti CF, Piticescu RM, Valero T, Kintzios S, Buruiana T. Synthesis and characterization of acid polyurethane—hydroxyapatite composites for biomedical applications. J Compos Mater. 2012;. doi: 10.1177/0021998312443396.Google Scholar
  26. 26.
    Popescu LM, Piticescu RM, Rusti CF, Maly M, Danani A, Kintzios S, Valero Grinan MT. Preparation and characterization of new hybrid nanostructured thin films for biosensors design. Mater Lett. 2011;65:2032–5. doi: 10.1016/j.matlet.2011.04.028.CrossRefGoogle Scholar
  27. 27.
    Negroiu G, Piticescu RM, Chitanu GC, Mihailescu IN, Zdrentu L, Miroiu M. Biocompatibility evaluation of a novel hydroxyapatite-polymer coating for medical implants (in vitro tests). J Mater Sci Mater Med. 2008;19:1537–44. doi: 10.1007/s10856-007-3300-6.CrossRefGoogle Scholar
  28. 28.
    Bartczak D, Kanaras AG. Diacetylene-containing ligand as a new capping agent for the preparation of water-soluble colloidal nanoparticles of remarkable stability. Langmuir. 2010;26:7072–7. doi: 10.1021/la9044013.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • L. M. Popescu
    • 1
    • 2
  • R. M. Piticescu
    • 2
  • A. Antonelli
    • 3
  • C. F. Rusti
    • 2
  • E. Carboni
    • 3
  • C. Sfara
    • 3
  • M. Magnani
    • 3
  • V. Badilita
    • 2
  • E. Vasile
    • 4
  • R. Trusca
    • 4
  • T. Buruiana
    • 1
  1. 1.“Petru Poni” Institute of Macromolecular ChemistryIasiRomania
  2. 2.National R&D Institute for Non-Ferrous and Rare MetalsPantelimonRomania
  3. 3.Department of Biomolecular SciencesUniversity of Urbino “Carlo Bo”UrbinoItaly
  4. 4.S.C. METAV-CDBucharestRomania

Personalised recommendations