Journal of Materials Science: Materials in Medicine

, Volume 24, Issue 11, pp 2567–2575 | Cite as

Regeneration of critical bone defects with anionic collagen matrix as scaffolds

  • Fúlvio Borges Miguel
  • Aryon de Almeida Barbosa Júnior
  • Fabiana Lopes de Paula
  • Isabela Cerqueira Barreto
  • Gilberto Goissis
  • Fabiana Paim Rosa
Article
First Online:
Received:
Accepted:

Abstract

The aim of this study was to make a histomorphometric evaluation of the osteogenic potential of anionic collagen matrix as scaffolds; either crosslinked in glutaraldehyde or not cross-linked and, implanted in critical bone defects in rat calvaria. Seventy-two rats were randomly distributed in three groups: anionic collagen scaffolds treated for 24 h of selective hydrolysis (ACSH); anionic collagen scaffolds treated for 24 h of selective hydrolysis and 5 min of crosslinking in glutaraldehyde 0.05 % (ACSHGA); empty bone defect (Control), evaluated at the biological points of 15, 45, 90 and 120 days. The results showed that the biomaterials implanted were biocompatible and showed a high osteogenic potential. These biomaterials presented a speed of biodegradation compatible with bone neoformation, which was shown to be associated with angiogenesis inside the scaffolds at all biological points. The percentage of mineralization of ACSH (87 %) differed statistically from that found in ACSHGA (66 %). It was concluded that the regeneration of critical bone defect was more evident in anionic collagen without crosslinking (ACSH).

Keywords

Bone Defect Osteogenic Potential Biological Point Selective Hydrolysis Bone Edge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

The authors thank Cristina Vasconcelos for technical assistance and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Fúlvio Borges Miguel
    • 1
    • 2
  • Aryon de Almeida Barbosa Júnior
    • 1
  • Fabiana Lopes de Paula
    • 2
  • Isabela Cerqueira Barreto
    • 3
  • Gilberto Goissis
    • 4
  • Fabiana Paim Rosa
    • 3
  1. 1.Gonçalo Moniz Research CenterOswaldo Cruz FoundationSalvadorBrazil
  2. 2.Health Sciences Center of Federal University of the Recôncavo of BahiaSanto Antônio de JesusBrazil
  3. 3.Health Sciences Institute of Federal University of BahiaSalvadorBrazil
  4. 4.Biotech Biomédica®São CarlosBrazil

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