Collagen–cellulose composite thin films that mimic soft-tissue and allow stem-cell orientation

  • Terry W. J. Steele
  • Charlotte L. Huang
  • Evelyne Nguyen
  • Udi Sarig
  • Saranya Kumar
  • Effendi Widjaja
  • Joachim S. C. Loo
  • Marcelle Machluf
  • Freddy Boey
  • Zlata Vukadinovic
  • Andreas Hilfiker
  • Subbu S. Venkatraman
Article

Abstract

Mechanical properties of collagen films are less than ideal for biomaterial development towards musculoskeletal repair or cardiovascular applications. Herein, we present a collagen–cellulose composite film (CCCF) compared against swine small intestine submucosa in regards to mechanical properties, cell growth, and histological analysis. CCCF was additionally characterized by FE-SEM, NMR, mass spectrometry, and Raman Microscopy to elucidate its physical structure, collagen–cellulose composition, and structure activity relationships. Mechanical properties of the CCCF were tested in both wet and dry environments, with anisotropic stress–strain curves that mimicked soft-tissue. Mesenchymal stem cells, human umbilical vein endothelial cells, and human coronary artery smooth muscle cells were able to proliferate on the collagen films with specific cell orientation. Mesenchymal stem cells had a higher proliferation index and were able to infiltrate CCCF to a higher degree than small intestine submucosa. With the underlying biological properties, we present a collagen–cellulose composite film towards forthcoming biomaterial-related applications.

Graphical Abstract

Supplementary material

10856_2013_4940_MOESM1_ESM.tif (205 kb)
Fig. S11H-NMR of D2O rinse from CCCF. Glycerol (3.7-3.4 ppm) and benzene (7.35 ppm, internal standard) are labeled.. Right inset: Magnified view from 2.2-1.0 ppm, displaying CH2 peaks from fatty acid(s) or ester(s) present. (TIFF 205 kb)
10856_2013_4940_MOESM2_ESM.tif (53 kb)
Fig. S2Macrophage stimulating assay. PLGA: Poly-L-Lactic-co- Glycolic-acid (85 %:15 %); LPS: Lipopolysaccharide from Pseudomonas aeruginosa, 1 μg/mL. * CCCF and PLGA were non-significantly different from one another P < 0.05, ** Basal NO secretion was significantly lower (P < 0.01) than all tested groups. (TIFF 52 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Terry W. J. Steele
    • 1
  • Charlotte L. Huang
    • 1
  • Evelyne Nguyen
    • 1
  • Udi Sarig
    • 1
    • 2
  • Saranya Kumar
    • 1
  • Effendi Widjaja
    • 3
  • Joachim S. C. Loo
    • 1
  • Marcelle Machluf
    • 2
  • Freddy Boey
    • 1
  • Zlata Vukadinovic
    • 4
  • Andreas Hilfiker
    • 4
  • Subbu S. Venkatraman
    • 1
  1. 1.Division of Materials Technology, Materials and Science EngineeringNanyang Technological UniversitySingaporeSingapore
  2. 2.Faculty of Biotechnology and Food EngineeringTechnion—Israel Institute of TechnologyHaifaIsrael
  3. 3.Process Science and ModelingInstitute of Chemical and Engineering Sciences, Agency for Science, Technology and Research (A*STAR)SingaporeSingapore
  4. 4.Leibniz Research Labs for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery Hannover Medical School (MHH)Hans-Borst-Zentrum (HBZ)HannoverGermany

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