Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites

  • Gabriela E. Vargas
  • Luis A. Haro Durand
  • Vanesa Cadena
  • Marcela Romero
  • Rosa Vera Mesones
  • Mirza Mačković
  • Stefanie Spallek
  • Erdmann Spiecker
  • Aldo R. Boccaccini
  • Alejandro A. GorustovichEmail author


Angiogenesis is essential for tissue regeneration and repair. A growing body of evidence shows that the use of bioactive glasses (BG) in biomaterial-based tissue engineering (TE) strategies may improve angiogenesis and induce increased vascularization in TE constructs. This work investigated the effect of adding nano-sized BG particles (n-BG) on the angiogenic properties of bovine type I collagen/n-BG composites. Nano-sized (20–30 nm) BG particles of nominally 45S5 Bioglass® composition were used to prepare composite films, which were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vivo angiogenic response was evaluated using the quail chorioallantoic membrane (CAM) as an model of angiogenesis. At 24 h post-implantation, 10 wt% n-BG containing collagen films stimulated angiogenesis by increasing by 41 % the number of blood vessels branch points. In contrast, composite films containing 20 wt% n-BG were found to inhibit angiogenesis. This experimental study provides the first evidence that addition of a limited concentration of n-BG (10 wt%) to collagen films induces an early angiogenic response making selected collagen/n-BG composites attractive matrices for tissue engineering and regenerative medicine.


Vascular Endothelial Growth Factor Composite Film Bioactive Glass Transmission Electron Microscopy Investigation Collagen Film 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by the National Research Council of Argentina (Grant PIP CONICET 0184 to A.A.G.). The authors thank the German research council (Deutsche Forschungsgemeinschaft, DFG) for partial financial support of this work.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Gabriela E. Vargas
    • 1
    • 2
  • Luis A. Haro Durand
    • 1
  • Vanesa Cadena
    • 1
    • 2
  • Marcela Romero
    • 2
  • Rosa Vera Mesones
    • 2
  • Mirza Mačković
    • 3
    • 4
  • Stefanie Spallek
    • 3
    • 4
  • Erdmann Spiecker
    • 3
    • 4
  • Aldo R. Boccaccini
    • 3
  • Alejandro A. Gorustovich
    • 1
    Email author
  1. 1.Interdisciplinary Materials Group-IESIING-UCASAL, INTECIN UBA-CONICETSaltaArgentina
  2. 2.School of Natural Sciences, National University of SaltaSaltaArgentina
  3. 3.Department of Materials Science and Engineering, Institute of BiomaterialsUniversity of Erlangen-NurembergErlangenGermany
  4. 4.Center for Nanoanalysis and Electron Microscopy (CENEM)University of Erlangen-NurembergErlangenGermany

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