Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites
- 870 Downloads
Angiogenesis is essential for tissue regeneration and repair. A growing body of evidence shows that the use of bioactive glasses (BG) in biomaterial-based tissue engineering (TE) strategies may improve angiogenesis and induce increased vascularization in TE constructs. This work investigated the effect of adding nano-sized BG particles (n-BG) on the angiogenic properties of bovine type I collagen/n-BG composites. Nano-sized (20–30 nm) BG particles of nominally 45S5 Bioglass® composition were used to prepare composite films, which were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vivo angiogenic response was evaluated using the quail chorioallantoic membrane (CAM) as an model of angiogenesis. At 24 h post-implantation, 10 wt% n-BG containing collagen films stimulated angiogenesis by increasing by 41 % the number of blood vessels branch points. In contrast, composite films containing 20 wt% n-BG were found to inhibit angiogenesis. This experimental study provides the first evidence that addition of a limited concentration of n-BG (10 wt%) to collagen films induces an early angiogenic response making selected collagen/n-BG composites attractive matrices for tissue engineering and regenerative medicine.
KeywordsVascular Endothelial Growth Factor Composite Film Bioactive Glass Transmission Electron Microscopy Investigation Collagen Film
This study was supported by the National Research Council of Argentina (Grant PIP CONICET 0184 to A.A.G.). The authors thank the German research council (Deutsche Forschungsgemeinschaft, DFG) for partial financial support of this work.
- 5.Misra SK, Ansari T, Mohn D, Valappil SP, Brunner TJ, Stark WJ, Roy I, Knowles JC, Sibbons PD, Jones EV, Boccaccini AR, Salih V. Effect of nanoparticulate bioactive glass particles on bioactivity and cytocompatibility of poly(3-hydroxybutyrate) composites. J R Soc Interface. 2010;7:453–65.CrossRefGoogle Scholar
- 9.Vila OF, Bagó JR, Navarro M, Alieva M, Aguilar E, Engel E, Planell J, Rubio N, Blanco J. Calcium phosphate glass improves angiogenesis capacity of poly(lactic acid) scaffolds and stimulates differentiation of adipose tissue-derived mesenchymal stromal cells to the endothelial lineage. J Biomed Mater Res. 2012;. doi: 10.1002/jbm.a.34391.Google Scholar
- 16.Wahl DA, Czernuszka JT. Collagen-hydroxyapatite composites for hard tissue repair. Eur Cell Mater. 2006;11:43–56.Google Scholar
- 27.Singh S, Wu BM, Dunn JC. Delivery of VEGF using collagen-coated polycaprolactone scaffolds stimulates angiogenesis. J Biomed Mater Res A. 2012;100:720–7.Google Scholar
- 33.Parsons-Wingerter P, Lwai B, Yang MC, Elliot KE, Milaninia A, Redlitz A, Clark JI, Sage EH. A novel assay of angiogenesis in the quail chorioallantoic membrane: stimulation by bFGF and Inhibition by angiostatin according to fractal dimension and grid intersection. Microvasc Res. 1998;55:201–14.CrossRefGoogle Scholar
- 34.Brooks PC, Montgomery AM, Cheresh DA. Use of the 10-day-old chick embryo model for studying angiogenesis. Methods Mol Biol. 1999;129:257–69.Google Scholar
- 36.Baiguera S, Macchiarini P, Ribatti D. Chorioallantoic membrane for in vivo investigation of tissue-engineered construct biocompatibility. J Biomed Mater Res B Appl Biomater. 2012;100:1425–34.Google Scholar
- 38.Borselli C, Ungaro F, Oliviero O, d’Angelo I, Quaglia F, La Rotonda MI, Netti PA. Bioactivation of collagen matrices through sustained VEGF release from PLGA microspheres. J Biomed Mater Res A. 2010;92:94–102.Google Scholar
- 41.Steffens GC, Yao C, Prével P, Markowicz M, Schenck P, Noah EM, Pallua N. Modulation of angiogenic potential of collagen matrices by covalent incorporation of heparin and loading with vascular endothelial growth factor. Tissue Eng. 2004;10:1502–9.Google Scholar
- 42.Irvine SM, Cayzer J, Todd EM, Lun S, Floden EW, Negron L, Fisher JN, Dempsey SG, Alexander A, Hill MC, O’Rouke A, Gunningham SP, Knight C, Davis PF, Ward BR, May BC. Quantification of in vitro and in vivo angiogenesis stimulated by ovine forestomach matrix biomaterial. Biomaterials. 2011;32:6351–61.CrossRefGoogle Scholar
- 48.Sharma AK, Bury MI, Fuller NJ, Rozkiewicz DI, Hota PV, Kollhoff DM, Webber MJ, Tapaskar N, Meisner JW, Lariviere PJ, DeStefano S, Wang D, Ameer GA, Cheng EY. Growth factor release from a chemically modified elastomeric poly(1,8-octanediol-co-citrate) thin film promotes angiogenesis in vivo. J Biomed Mater Res, Part A. 2012;100A:561–70.CrossRefGoogle Scholar