Effect of solid freeform fabrication-based polycaprolactone/poly(lactic-co-glycolic acid)/collagen scaffolds on cellular activities of human adipose-derived stem cells and rat primary hepatocytes

  • Jin-Hyung Shim
  • Arthur Joon Kim
  • Ju Young Park
  • Namwoo Yi
  • Inhye Kang
  • Jaesung Park
  • Jong-Won Rhie
  • Dong-Woo Cho


Highly biocompatible polycaprolactone (PCL)/poly(lactic-co-glycolic acid) (PLGA)/collagen scaffolds in which the PCL/PLGA collagen solution was selectively dispensed into every other space between the struts were fabricated using solid freeform fabrication (SFF) technology, as we described previously. The objective of this study was to evaluate and compare the PCL/PLGA/collagen scaffolds (group 3) with PCL/PLGA-only scaffolds (group 1) and PCL/PLGA scaffolds with collagen by the dip-coating method (group 2) using human adipose-derived stem cells (hASCs) and rat primary hepatocytes. The selectively dispensed collagen formed a three-dimensional (3D) network of nanofibers in group 3, as observed by scanning electron microscopy. The compressive strength and modulus of group 3 were approximately 140 and 510 times higher, respectively, than those of a sponge-type collagen scaffold whose weak mechanical properties were regarded as a critical drawback. Proliferation and osteogenic differentiation of hASCs were promoted significantly in group 3 compared to groups 1 and 2. In addition, we found that the viability and albumin secretion ability of rat primary hepatocytes were highly retained for 10 days in group 3 but not group 1. Interestingly, hepatocyte aggregation, which enhances hepatic function through cell–cell interactions, was observed particularly in group 3. In conclusion, group 3, in which the collagen was selectively dispensed in the 3D space of the porous PCL/PLGA framework, will be a promising 3D scaffold for culturing various cell types.


Compressive Strength Osteogenic Differentiation Collagen Solution Primary Hepatocyte Hepatocyte Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MEST) (Nos. 2012-0001235, and 2011-0028845).

Supplementary material

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Jin-Hyung Shim
    • 1
  • Arthur Joon Kim
    • 2
  • Ju Young Park
    • 3
  • Namwoo Yi
    • 1
  • Inhye Kang
    • 1
  • Jaesung Park
    • 1
  • Jong-Won Rhie
    • 4
  • Dong-Woo Cho
    • 1
    • 3
  1. 1.Department of Mechanical EngineeringPohang University of Science and Technology (POSTECH)PohangSouth Korea
  2. 2.Harvard School of Dental MedicineBostonUSA
  3. 3.Division of Integrative Biosciences and BiotechnologyPohang University of Science and Technology (POSTECH)PohangSouth Korea
  4. 4.Department of Plastic SurgeryCollege of Medicine, The Catholic University of KoreaBucheonSouth Korea

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