Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) based composite materials

  • R. Costa
  • C. Ribeiro
  • A. C. Lopes
  • P. Martins
  • V. Sencadas
  • R. Soares
  • S. Lanceros-Mendez


Electroactive materials can be taken to advantage for the development of sensors and actuators as well as for novel tissue engineering strategies. Composites based on poly(vinylidene fluoride), PVDF, have been evaluated with respect to their biological response. Cell viability and proliferation were performed in vitro both with Mesenchymal Stem Cells differentiated to osteoblasts and Human Fibroblast Foreskin 1. In vivo tests were also performed using 6-week-old C57Bl/6 mice. It was concluded that zeolite and clay composites are biocompatible materials promoting cell response and not showing in vivo pro-inflammatory effects which renders both of them attractive for biological applications and tissue engineering, opening interesting perspectives to development of scaffolds from these composites. Ferrite and silver nanoparticle composites decrease osteoblast cell viability and carbon nanotubes decrease fibroblast viability. Further, carbon nanotube composites result in a significant increase in local vascularization accompanied an increase of inflammatory markers after implantation.


Nitric Oxide Mesenchymal Stem Cell PVDF CoFe2O4 Vinylidenefluoride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work is funded by FEDER funds through the “Programa Operacional Factores de Competitividade — COMPETE” and by national funds by FCT— Fundação para a Ciência e a Tecnologia, project references NANO/NMed-SD/0156/2007. C. Ribeiro thanks the INL for a PhD grant. V.Sencadas thanks the FCT for the SFRH/BPD/63148/2009 grants. The authors also thank the support of the COST Action MP1003, 2010 ‘European Scientific Network for Artificial Muscles’ (ESNAM).


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • R. Costa
    • 1
  • C. Ribeiro
    • 2
    • 4
  • A. C. Lopes
    • 2
  • P. Martins
    • 2
    • 4
  • V. Sencadas
    • 2
    • 3
    • 4
  • R. Soares
    • 1
  • S. Lanceros-Mendez
    • 2
    • 4
  1. 1.Department of Biochemistry (U38-FCT), Faculty of MedicineUniversity of PortoPortoPortugal
  2. 2.Centro/Departamento de FísicaUniversidade do Minho, Campus de GualtarBragaPortugal
  3. 3.Escola Superior de TecnologiaInstituto Politécnico do Cávado e do Ave, Campus do IPCABarcelosPortugal
  4. 4.INL—International Iberian Nanotechnology LaboratoryBragaPortugal

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