Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) based composite materials
- 797 Downloads
Electroactive materials can be taken to advantage for the development of sensors and actuators as well as for novel tissue engineering strategies. Composites based on poly(vinylidene fluoride), PVDF, have been evaluated with respect to their biological response. Cell viability and proliferation were performed in vitro both with Mesenchymal Stem Cells differentiated to osteoblasts and Human Fibroblast Foreskin 1. In vivo tests were also performed using 6-week-old C57Bl/6 mice. It was concluded that zeolite and clay composites are biocompatible materials promoting cell response and not showing in vivo pro-inflammatory effects which renders both of them attractive for biological applications and tissue engineering, opening interesting perspectives to development of scaffolds from these composites. Ferrite and silver nanoparticle composites decrease osteoblast cell viability and carbon nanotubes decrease fibroblast viability. Further, carbon nanotube composites result in a significant increase in local vascularization accompanied an increase of inflammatory markers after implantation.
KeywordsNitric Oxide Mesenchymal Stem Cell PVDF CoFe2O4 Vinylidenefluoride
This work is funded by FEDER funds through the “Programa Operacional Factores de Competitividade — COMPETE” and by national funds by FCT— Fundação para a Ciência e a Tecnologia, project references NANO/NMed-SD/0156/2007. C. Ribeiro thanks the INL for a PhD grant. V.Sencadas thanks the FCT for the SFRH/BPD/63148/2009 grants. The authors also thank the support of the COST Action MP1003, 2010 ‘European Scientific Network for Artificial Muscles’ (ESNAM).
- 4.Lovinger AJ. Developments in crystalline polymers. London: Elsevier Applied Science; 1982.Google Scholar
- 5.Nalwa HS. Ferroelectric polymers: chemistry, physics and applications. New York: Marcel Dekker, Inc.; 1995.Google Scholar
- 7.Rodrigues MT, Gomes ME, Mano JF, Reis RL. beta-PVDF membranes induce cellular proliferation and differentiation in static and dynamic conditions. In: Marques AT, Silva AF, Baptista APM, Sa C, Alves F, Malheiros LF, et al., editors. Advanced Materials Forum Iv. Materials Science Forum. Stafa-Zurich: Trans Tech; 2008. p. 72–6.Google Scholar
- 19.Guan GP, Bai L, Zuo BQ, Li MZ, Wu ZY, Li YL. Scaffolds decorated by in vivo environment improve cell proliferation and wound healing. New York: IEEE; 2009. 1-4.Google Scholar
- 20.Rickert D, Moses MA, Lendlein A, Kelch S, Franke RP. The importance of angiogenesis in the interaction between polymeric biomaterials and surrounding tissue. Clin Hemorheol Microcirc. 2003;28(3):175–81.Google Scholar
- 26.Firmino Mendes S, Costa C, Sencadas V, Serrado Nunes J, Costa P, Gregorio R, et al. Effect of the ceramic grain size and concentration on the dynamical mechanical and dielectric behavior of poly(vinilidene fluoride)/Pb(Zr0.53Ti0.47)O3 composites. Appl Phys A: Mater. 2009;96(4):899–908. doi: 10.1007/s00339-009-5141-2.CrossRefGoogle Scholar
- 27.Costa P, Silva J, Sencadas V, Costa CM, van Hattum FWJ, Rocha JG, et al. The effect of fibre concentration on the alpha to beta-phase transformation, degree of crystallinity and electrical properties of vapor grown carbon nanofibre/poly(vinylidene fluoride) composites. Carbon. 2009;47(11):2590–9. doi: 10.1016/j.carbon.2009.05.011.CrossRefGoogle Scholar
- 28.Miranda D, Sencadas V, Sanchez-Iglesias A, Pastoriza-Santos I, Liz-Marzan LM, Ribelles JLG, et al. Influence of silver nanoparticles concentration on the alpha- to beta-phase transformation and the physical properties of silver nanoparticles doped poly(vinylidene fluoride) nanocomposites. J Nanosci Nanotechnol. 2009;9(5):2910–6. doi: 10.1166/jnn.2009.208.CrossRefGoogle Scholar
- 32.Shastri VR, Rahman N, Martin I, Robert S Langer Jr. Electro active materials for stimulation of biological activity of bone marrow stromal cells. US Patent 6,190,893. 20 Feb 2001.Google Scholar
- 34.Williamson MR, Black R, Kielty C. PCL–PU composite vascular scaffold production for vascular tissue engineering: attachment, proliferation and bioactivity of human vascular endothelial cells. Biomaterials. 2006;27(19):3608–16.Google Scholar
- 39.Serrano MC, Pagani R, Vallet-Regi M, Pena J, Comas JV, Portoles MT. Nitric oxide production by endothelial cells derived from blood progenitors cultured on NaOH-treated polycaprolactone films: A biofunctionality study. Acta Biomater. 2009;5(6):2045–53. doi: 10.1016/j.actbio.2009.02.034.CrossRefGoogle Scholar