Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones
- 889 Downloads
Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.
KeywordsAlveolar Bone Autologous Blood Osteoporotic Bone Haversian Canal Cement Line
The research presented in this paper was supported by the Ministry of Education and Science of the Republic of Serbia under the Project No. III45004 and by the NIH/NIDCR grant K99-DE021416. Confocal microscopy data for this study were acquired at the Nikon Imaging Center at University of California, San Francisco. FE-SEM images were obtained by courtesy of Dr. Srečo Škapin from Jožef Stefan Institute. Authors would also like to thank MSc. Ljilijana Veselinović for XRD measurements.
- 2.Pollähne W, Pfeifer M, Lazarescu A, Minne HW. Osteoporose: bildgebende diagnostik. Medizin im Bild. 1996;3:37–44.Google Scholar
- 7.Le Geros RZ, Craig RG. Strategies to affect bone remodeling: osteointegration. J Bone Miner Res. 1993;8(2):S583–96.Google Scholar
- 8.Ajdukovic Z, Ignjatovic N, Petrovic D, Uskokovic D. Substitution of osteoporotic alveolar bone by biphasic calcium phosphate/poly –DL-lactide–co-glycolide biomaterials. J Biomater Appl. 2007;21(3):317–28.Google Scholar
- 13.Ignjatovic N, Ajdukovic Z, Savic V, Uskokovic D. Size effect of calcium phosphate coated with poly-(DL-lactide-co-glycolide) on healing processes in bone reconstruction. J Biomed Mater Res B Appl Biomater. 2010;94B:108–17.Google Scholar
- 30.Macleod I, Heath N. Cone-beam computed tomography (CBCT) in dental practice. Dent Update. 2008;35:590–8.Google Scholar
- 35.Horev-Azaria L, Kirkpatrick CJ, Korenstein R, Marche PN, Maimon O, Ponti J, Romano R, Rossi F, Golla-Schindler U, Sommer D, Uboldi C, Unger RE, Villiers C. Predictive toxicology of cobalt nanoparticles and ions: comparative in vitro study of different cellular models using methods of knowledge discovery from data. Toxicol Sci. 2011;122(2):489–501.CrossRefGoogle Scholar
- 38.de Lima IR, Alves GG, Soriano CA, Campaneli AP, Gasparoto TH, Ramos ES Jr, de Sena LÁ, Rossi AM, Granjeiro JM. Understanding the impact of divalent cation substitution on hydroxyapatite: an in vitro multiparametric study on biocompatibility. J Biomed Mater Res A. 2011;98(3):351–8. doi: 10.1002/jbm.a.33126.Google Scholar
- 41.Hallab NJ, Chan FW, Harper ML, Quantifying subtle but persistent peri-spine inflammation in vivo to submicron cobalt-chromium alloy particles, Eur Spine J, 2012 Mar 10. [Epub ahead of print].Google Scholar
- 43.D.B. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons. Biomaterials science. An Introduction to Materials in Medicine, 2nd Edition, Elsevier Academic Press San Diego, California, USA 2004:23-40;Google Scholar
- 44.Seibel JM. Biochemical markers of bone turnover part II: clinical applications in the management of osteoporosis. Clin Biochem Rev. 2006;27(3):123–38.Google Scholar