Electrospun polycaprolactone scaffolds with tailored porosity using two approaches for enhanced cellular infiltration

  • Nicole E. ZanderEmail author
  • Joshua A. Orlicki
  • Adam M. Rawlett
  • Thomas P. BeebeJr.


The impact of mat porosity of polycaprolactone (PCL) electrospun fibers on the infiltration of neuron-like PC12 cells was evaluated using two different approaches. In the first method, bi-component aligned fiber mats were fabricated via the co-electrospinning of PCL with polyethylene oxide (PEO). Variation of the PEO flow rate, followed by selective removal of PEO from the PCL/PEO mesh, allowed for control of the porosity of the resulting scaffold. In the second method, aligned fiber mats were fabricated from various concentrations of PCL solutions to generate fibers with diameters between 0.13 ± 0.06 and 9.10 ± 4.1 μm. Of the approaches examined, the variation of PCL fiber diameter was found to be the better method for increasing the infiltration of PC12 cells, with the optimal infiltration into the ca. 1.5-mm-thick meshes observed for the mats with the largest fiber diameters, and hence largest pore sizes.


PC12 Cell Fiber Diameter Electrospun Fiber Cellular Infiltration Infiltration Depth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York (outside the USA) 2012

Authors and Affiliations

  • Nicole E. Zander
    • 1
    • 2
    Email author
  • Joshua A. Orlicki
    • 1
  • Adam M. Rawlett
    • 1
  • Thomas P. BeebeJr.
    • 2
  1. 1.US Army Research LaboratoryWeapons and Materials Research DirectorateAberdeen Proving GroundUSA
  2. 2.Department of Chemistry and BiochemistryUniversity of DelawareNewarkUSA

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