Bioactive glass-derived trabecular coating: a smart solution for enhancing osteointegration of prosthetic elements

  • Chiara Vitale-Brovarone
  • Francesco Baino
  • Francesca Tallia
  • Cristina Gervasio
  • Enrica Verné


In this work, the use of foam-like glass–ceramic scaffolds as trabecular coatings on ceramic prosthetic devices to enhance implant osteointegration is proposed. The feasibility of this innovative device was explored in a simplified, flat geometry: glass–ceramic scaffolds, prepared by polymeric sponge replication and mimicking the trabecular architecture of cancellous bone, were joined to alumina square substrates by a dense glass coating (interlayer). The role played by different formulations of starting glasses was examined, with particular care to the effect on the mechanical properties and bioactivity of the final coating. Microindentations at the coating/substrate interface and tensile tests were performed to evaluate the bonding strength between the sample’s components. In vitro bioactive behaviour was assessed by soaking in simulated body fluid and evaluating the apatite formation on the surface and inside the pores of the trabecular coating. The concepts disclosed in the present study can have a significant impact in the field of implantable devices, suggesting a valuable alternative to traditional, often invasive bone-prosthesis fixation.


Simulated Body Fluid Bioactive Glass Wollastonite Alumina Substrate Glass Powder 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was partly funded by the EU Network of Excellence project “Knowledge-based Multicomponent Materials for Durable and Safe Performance” (KMM-NoE, NMP3-CT-2004-502243). Prof. Milena Salvo is gratefully acknowledged for fruitful discussions.


  1. 1.
    Soballe K, Overgaard S, Hansen ES, Brokstedt-Rasmussen H, Lind M, Bunger C. A review of ceramic coatings for implant fixation. J Long Term Eff Med Implants. 1999;9:131–51.Google Scholar
  2. 2.
    Manley MT, Sutton K. Bearings of the future for total hip arthroplasty. J Arthroplasty. 2008;23:S47–50.CrossRefGoogle Scholar
  3. 3.
    Hench LL, Anderson O. Bioactive glass coatings. In: Hench LL, Wilson J, editors. An introduction to bioceramics. Singapore: World Scientific; 1993. p. 239–60.CrossRefGoogle Scholar
  4. 4.
    Verné E, Bosetti M, Vitale-Brovarone C, Moisescu C, Lupo F, Spriano S, Cannas M. Fluoroapatite glass–ceramic coatings on alumina: structural, mechanical and biological characterisation. Biomaterials. 2002;23:3395–403.CrossRefGoogle Scholar
  5. 5.
    Lopez-Esteban S, Saiz E, Fujino S, Oku T, Suganuma K, Tomsia AP. Bioactive glass coating for orthopaedic metallic implants. J Eur Ceram Soc. 2003;23:2921–30.CrossRefGoogle Scholar
  6. 6.
    Vitale-Brovarone C, Verné E. SiO2–CaO–K2O coatings on alumina and Ti6Al4V substrates for biomedical applications. J Mater Sci Mater Med. 2005;16:863–71.CrossRefGoogle Scholar
  7. 7.
    Verné E, Vitale-Brovarone C, Moisescu C. Glazing of alumina by a fluoroapatite-containing glass–ceramic. J Mater Sci. 2005;40:1209–15.CrossRefGoogle Scholar
  8. 8.
    Bigi A, Boanini E, Bracci B, Facchini A, Panzavolta S, Segatti F, Sturba L. Monocrystalline hydroxyapatite coatings on titanium: a new fast biomimetic method. Biomaterials. 2005;26:4085–9.CrossRefGoogle Scholar
  9. 9.
    Fathi MH, Doostmohammadi A. Bioactive glass nanopowder and bioglass coating for biocompatibility improvement of metallic implant. J Mater Processing Technol. 2009;209:1385–91.CrossRefGoogle Scholar
  10. 10.
    Wang X, Li X, Onuma K, Ito A, Sogo Y, Kosuge K, Oyane A. Mesoporous bioactive glass coatings on stainless steel for enhanced cell activity, cytoskeletal organization and AsMg immobilization. J Mater Chem. 2010;20:6437–45.CrossRefGoogle Scholar
  11. 11.
    Sun L, Berndt CC, Gross KA, Kucuk A. Material fundamentals and clinical performance of plasma-sprayed hydroxyapatite coatings: a review. J Biomed Mater Res Appl Biomater. 2001;58:570–92.CrossRefGoogle Scholar
  12. 12.
    Yang Y, Kim KH, Ong JL. A review on calcium phosphate coatings produced using a sputtering process—an alternative to plasma spraying. Biomaterials. 2005;26:327–37.CrossRefGoogle Scholar
  13. 13.
    Boccaccini AR, Keim S, Ma R, Li Y, Zhitomirsky I. Electrophoretic deposition of biomaterials. J R Soc Interface. 2010;7:S581–613.CrossRefGoogle Scholar
  14. 14.
    Klein CPAT, Wolke JGC, De Groot K. Stability of calcium phosphate ceramics and plasma sprayed coatings. In: Hench LL, Wilson J, editors. An introduction to bioceramics. Singapore: World Scientific; 1993. p. 199–222.CrossRefGoogle Scholar
  15. 15.
    Dorozhkin SV. Bioceramics of calcium orthophosphates. Biomaterials. 2010;31:1465–85.CrossRefGoogle Scholar
  16. 16.
    Tomsia AP, Saiz E, Song J, Bertozzi CR. Biomimetic bonelike composites and novel bioactive glass coatings. Adv Eng Mater. 2005;7:999–1004.CrossRefGoogle Scholar
  17. 17.
    Hench LL, Splinter RJ, Allen WC, Greenlee TK. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res. 1972;2:117–41.Google Scholar
  18. 18.
    Hench LL. The story of Bioglass®. J Mater Sci Mater Med. 2006;17:967–78.CrossRefGoogle Scholar
  19. 19.
    Hench LL. Genetic design of bioactive glasses. J Eur Ceram Soc. 2009;29:1257–65.CrossRefGoogle Scholar
  20. 20.
    Hoppe A, Guldal NS, Boccaccini AR. A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials. 2011;32:2757–74.CrossRefGoogle Scholar
  21. 21.
    Foppiano S, Marshall SJ, Marshall GW, Saiz E, Tomsia AP. Bioactive glass coatings affect the behavior of osteoblast-like cells. Acta Biomater. 2007;3:765–71.CrossRefGoogle Scholar
  22. 22.
    Gerhardt LC, Boccaccini AR. Bioactive glass and glass–ceramic scaffolds for bone tissue engineering. Materials. 2010;3:3867–910.CrossRefGoogle Scholar
  23. 23.
    Baino F, Vitale-Brovarone C. Three-dimensional glass-derived scaffolds for bone tissue engineering: current trends and forecasts for the future. J Biomed Mater Res A. 2011;97:514–35.Google Scholar
  24. 24.
    Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005;26:5474–91.CrossRefGoogle Scholar
  25. 25.
    Verné E, Vitale-Brovarone C, Robiglio L, Baino F. Single-piece ceramic prosthesis elements. WO 2008/146322 A2.Google Scholar
  26. 26.
    Vitale-Brovarone C, Verné E, Robiglio L, Appendino P, Bassi F, Martinasso G, Muzio G, Canuto R. Development of glass–ceramic scaffolds for bone tissue engineering: characterisation, proliferation of human osteoblasts and nodule formation. Acta Biomater. 2007;3:199–208.CrossRefGoogle Scholar
  27. 27.
    Vitale-Brovarone C, Baino F, Verné E. High strength bioactive glass-ceramic scaffolds for bone regeneration. J Mater Sci Mater Med. 2009;20:643–53.CrossRefGoogle Scholar
  28. 28.
    Verné E, Miola M, Vitale-Brovarone C, Cannas M, Gatti S, Fucale G, Maina G, Massé A, Di Nunzio S. Surface silver-doping of biocompatibile glass to induce antibacterial properties. Part I: massive glass. J Mater Sci Mater Med. 2009;20:733–40.CrossRefGoogle Scholar
  29. 29.
    Baino F, Ferraris M, Bretcanu O, Verné E, Vitale-Brovarone C. Optimization of composition, structure and mechanical strength of bioactive 3-D glass-ceramic scaffolds for bone substitution. J Biomater Appl. 2011 doi: 10.1177/0885328211429193.
  30. 30.
    Priven AI. General method for calculating the properties of oxide glass and glass forming melts from their composition and temperature. Glass Technol. 2004;45:244–54.Google Scholar
  31. 31.
    Chalker PR, Bull SJ, Rickerby DS. A review of the methods for the evaluation of coating-substrate adhesion. Mater Sci Eng A. 1991;140:583–92.CrossRefGoogle Scholar
  32. 32.
    Gomez-Vega JM, Saiz E, Tomsia AP, Marshall GW, Marshall SJ. Bioactive glass coatings with hydroxyapatite and Bioglass® particle on Ti-based implants. 1. Processing. Biomaterials. 2000;21:105–11.CrossRefGoogle Scholar
  33. 33.
    ASTM F1538-03. Standard specification for glass and glass ceramic biomaterials for implantation;2009.Google Scholar
  34. 34.
    ASTM C633-01. Standard test method for adhesion or cohesion strength of thermal spray coatings;2008.Google Scholar
  35. 35.
    Kokubo T, Takadama H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials. 2006;27:2907–15.CrossRefGoogle Scholar
  36. 36.
    Kokubo T, Ito S, Sakka S, Yamamuro T. Formation of a high-strength bioactive glass–ceramic in the system MgO–CaO–SiO2–P2O5. J Mater Sci. 1986;21:536–40.CrossRefGoogle Scholar
  37. 37.
    Xue W, Liu X, Zheng XB, Ding C. In vivo evaluation of plasma-sprayed wollastonite coating. Biomaterials. 2005;26:3455–60.CrossRefGoogle Scholar
  38. 38.
    Erol M, Kucukbayrak S, Ersoy-Mericboyu A. Influence of particle size on the crystallization kinetics of glasses produced from waste materials. J Non-Cryst Solids. 2011;357:211–9.CrossRefGoogle Scholar
  39. 39.
    ISO 13779-4. Implants for surgery—Hydroxyapatite—Part 4: Determination of coating adhesion strength;2002.Google Scholar
  40. 40.
    Chen QZ, Thompson ID, Boccaccini AR. 45S5 bioglass®-derived glass–ceramic scaffolds for bone tissue engineering. Biomaterials. 2006;27:2414–25.CrossRefGoogle Scholar
  41. 41.
    Bretcanu O, Chen Q, Misra SK, Boccaccini AR, Verné E, Vitale-Brovarone C. Biodegradable polymer coated 45S5 Bioglass-derived glass–ceramic scaffolds for bone tissue engineering. Glass Tech Eur J Glass Sci Tech A. 2007;48:227–34.Google Scholar
  42. 42.
    Kokubo T, Kushitani H, Ohtsuki C, Sakka S, Yamamuro T. Effects of ions dissolved from bioactive glass–ceramics on surface apatite formation. J Mater Sci Mater Med. 1993;4:1–4.CrossRefGoogle Scholar
  43. 43.
    Verné E, Ferraris S, Vitale-Brovarone C, Spriano S, Bianchi CL, Naldoni A, Morra M, Cassinelli C. Alkaline phosphatase grafting on bioactive glasses and glass–ceramics. Acta Biomater. 2010;6:229–40.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Chiara Vitale-Brovarone
    • 1
  • Francesco Baino
    • 1
  • Francesca Tallia
    • 1
  • Cristina Gervasio
    • 1
  • Enrica Verné
    • 1
  1. 1.Institute of Materials Engineering and Physics, Applied Science and Technology DepartmentPolitecnico di TorinoTurinItaly

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