Development of strong and bioactive calcium phosphate cement as a light-cure organic–inorganic hybrid

Article

Abstract

In this research, light cured calcium phosphate cements (LCCPCs) were developed by mixing a powder phase (P) consisting of tetracalcium phosphate and dicalcium phosphate and a photo-curable resin phase (L), mixture of hydroxyethylmethacrylate (HEMA)/poly acrylic-maleic acid at various P/L ratios of 2.0, 2.4 and 2.8 g/mL. Mechanical strength, phase composition, chemical groups and microstructure of the cured cements were evaluated at pre-set times, i.e. before and after soaking in simulated body fluid (SBF). The proliferation of Rat-derived osteoblastic cells onto the LCCPCs as well as cytotoxicity of cement extracts were determined by cell counting and 3-{4,5-dimethylthiazol-2yl}-2,5-diphenyl-2H-tetrazolium bromide assay after different culture times. It was estimated from Fourier transforming infrared spectra of cured cements that the setting process is ruled by polymerization of HEMA monomers as well as formation of calcium poly-carboxylate salts. Microstructure of the cured cements consisted of calcium phosphate particles surrounded by polymerized resin phase. Formation of nano-sized needlelike calcium phosphate phase on surfaces of cements with P/L ratios of 2.4 and 2.8 g/mL was confirmed by scanning electron microscope images and X-ray diffractometry (XRD) of the cured specimen soaked in SBF for 21 days. Also, XRD patterns revealed that the formed calcium phosphate layer was apatite phase in a poor crystalline form. Biodegradation of the cements was confirmed by weight loss, change in molecular weight of polymer and morphology of the samples after different soaking periods. The maximum compressive strength of LCCPCs governed by resin polymerization and calcium polycarboxylate salts formation was about 80 MPa for cement with P/L ratio of 2.8 g/mL, after incubation for 24 h. The strength of all cements decreased by decreasing P/L ratio as well as increasing soaking time. The preliminary cell studies revealed that LCCPCs could support proliferation of osteoblasts cultured on their surfaces and no cytotoxic effect was observed for the extracts of them.

Notes

Acknowledgments

Authors wish to acknowledge Pasteur Institute and Medical College of Shahid Beheshti University for the cell experiments.

References

  1. 1.
    Charnley J. Anchorage of femoral head prosthesis to the shaft of the femur. J Bone J Surg. 1960;42B:28–30.Google Scholar
  2. 2.
    Jasty M, Maloney WJ, Bragdon CR, Haire T, Harris WH. Histomorphological studies of the long-term skeletal responses to well fixed cemented femoral component. J Bone J Surg. 1990;72A:1220–5.Google Scholar
  3. 3.
    Freeman MAR, Bradley GW, Revell PA. Observation upon the interface between bone and polymethylmethacrylate cement. J Bone J Surg. 1982;64B:489–93.Google Scholar
  4. 4.
    Dickens SH, Kelly SR, Flaim GM, Giuseppetti AA. Dentin adhesion and microleakage of a resin-based calcium phosphate pulp capping and basing cement. Eur J Oral Sci. 2004;112:452–7.CrossRefGoogle Scholar
  5. 5.
    Walsh WR, Svehla MJ, Russell J, Saito M, Nakashima T, Gillies RM, et al. Cemented fixation with PMMA or Bis-GMA resin hydroxyapatite cement: effect of implant surface roughness. Biomaterials. 2004;25:4929–34.CrossRefGoogle Scholar
  6. 6.
    Dalby MJ, Di Silvio L, Harper EJ, Bonfield W. Initial interaction of osteoblasts with the surface of a hydroxyapatite–poly(methylmethacrylate) cement. Biomaterials. 2001;22:1739–47.CrossRefGoogle Scholar
  7. 7.
    Fujita H, Nakamura T, Tamura J, Kobayashi M, Katsura Y, Kokubo T, et al. Bioactive bone cement: effect of the amount of glass-ceramic powder on bone-bonding strength. J Biomed Mater Res. 1998;40:145–52.CrossRefGoogle Scholar
  8. 8.
    LeGeros RZ, Chohayeb A, Shulman A. Apatitic calcium phosphates: possible restorative materials. J Dent Res. 1982;61:343–51.Google Scholar
  9. 9.
    Brown WE, Chow LC. A new calcium phosphate water setting cement. In: Brown PW, editor. Cements research progress. Westerville: American Ceramic Society; 1986. p. 352–79.Google Scholar
  10. 10.
    Friedman CD, Costantino PD, Jones K, Chow LC, Pelzer HJ, Sisson GA. Hydroxyapatite cement: II, obliteration and reconstruction of the cat frontal sinus. Arch Otolaryngol Head Neck Surg. 1991;117:385–9.CrossRefGoogle Scholar
  11. 11.
    Costantino PD, Friedman CD, Jones K, Chow LC, Sisson GA. Experimental hydroxyapatite cement cranioplasty. Plast Reconstr Surg. 1992;90:174–91.CrossRefGoogle Scholar
  12. 12.
    Takagi S, Chow LC, Markovic M, Friedman CD, Costantino PD. Morphological and phase characterizations of retrieved calcium phosphate cement implants. J Biomed Mater Res (Appl Biomater). 2001;58:36–41.CrossRefGoogle Scholar
  13. 13.
    Bohner M, Gbureck U, Barralet JE. Technological issues for the development of more efficient calcium phosphate bone cements: a critical assessment. Biomaterials. 2005;26:6423–9.CrossRefGoogle Scholar
  14. 14.
    Ayad MF, Bahannan SA, Rosenstiel SF. Morphological characteristics of the interface between resin composite and glass-ionomer cement to thin-walled roots: a microscopic investigation. Am J Dent. 2010;23:103–7.Google Scholar
  15. 15.
    Jakubiak J, Allonas X, Fouassier JP, Sionkowska A, Andrzejewsk E, Linden LA, Rabek JF. Camphorquinone-amines photoinitating systems for the initiation of free radical polymerization. Polymer. 2003;44:5219–26.CrossRefGoogle Scholar
  16. 16.
    Hesaraki S, Moztarzadeh F, Sharifi D. Formation of interconnected macropores in apatitic calcium phosphate bone cement with use of an effervescent additive. J Biomed Mater Res. 2007;83A:80–7.CrossRefGoogle Scholar
  17. 17.
    Hesaraki S, Sharifi D, Nemati R, Nezafati N. Preparation and characterisation of calcium phosphate cement made by poly(acrylic/itaconic) acid. Adv Appl Ceram. 2009;108:106–10.CrossRefGoogle Scholar
  18. 18.
    Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro TJ. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. J Biomed Mater Res. 1990;24:721–34.CrossRefGoogle Scholar
  19. 19.
    Hesaraki S, Alizadeh M, Nazarian H, Sharifi D. Physico-chemical and in vitro biological evaluation of strontium/calcium silicophosphate glass. J Mater Sci Mater Med. 2010;21:695–705.CrossRefGoogle Scholar
  20. 20.
    Nourmohammadi J, Sadrnezhaad SK, Ghader AB. Bone-like apatite layer formation on the new resin-modified glass-ionomer cement. J Mater Sci Mater Med. 2008;19:3507–14.CrossRefGoogle Scholar
  21. 21.
    Hesaraki S, Moztarzadeh F, Solati-Hashjin M. Phase evaluation of an effervescent-added apatitic calcium phosphate bone cement. J Biomed Mater Res B. 2006;79:203–9.Google Scholar
  22. 22.
    Barralet JE, Grover L, Gaunt T, Wright AJ, Gibson IR. Preparation of macroporous calcium phosphate cement tissue engineering scaffold. Biomaterials. 2002;23:3063–72.CrossRefGoogle Scholar
  23. 23.
    Yuasa T, Miyamoto Y, Ishikawa K, Takechi M, Nagayama M, Suzuki K. In vitro resorption of three apatite cements with osteoclasts. J Biomed Mater Res. 2001;54:344–50.CrossRefGoogle Scholar
  24. 24.
    Hesaraki S, Nemati R. Cephalexin-loaded injectable macroporous calcium phosphate bone cement. J Biomed Mater Res B. 2009;89B:342–52.CrossRefGoogle Scholar
  25. 25.
    Hesaraki S, Zamanian A, Nazarian H. Physical and physicochemical evaluation of calcium phosphate bone cement made using human derived blood plasma. Adv Appl Ceram. 2009;108:253–60.CrossRefGoogle Scholar
  26. 26.
    Ratner BD, Hoffman AS, Schoen FJ, Lemons JE. Biomaterials science: an introduction to materials in medicine. Chapter 2: classes of materials used in medicine. San Diego: Academic; 1996.Google Scholar
  27. 27.
    Lisch W, Wasielica-Poslednik J, Lisch C, Saikia P, Pitz S. Contact lens-induced regression of Lisch epithelial corneal dystrophy. Cornea. 2010;29:342–5.CrossRefGoogle Scholar
  28. 28.
    Terada S, Suzuki K, Nozaki M, Okano T, Takemura N. Anti-thrombogenic effects of 2-hydroxyethylmethacrylate-styrene block copolymer and argatroban in synthetic small-caliber vascular grafts in a rabbit inferior vena cava model. J Reconstr Microsurg. 1997;13:9–16.CrossRefGoogle Scholar
  29. 29.
    Migliaresi C, Nicolais L. Composite materials for biomedical applications. Int J Artif Organs. 1980;3:114–8.Google Scholar
  30. 30.
    Morra M. Acid–base properties of adhesive dental polymers. Dent Mater. 1993;9:375–8.CrossRefGoogle Scholar
  31. 31.
    Jones DW, Rizkalla AS. Characterization of experimental composite biomaterials. J Biomed Mater Res. 1996;33:89–100.CrossRefGoogle Scholar
  32. 32.
    Deb S, Aiyathurai L, Roether JA, Luklinska ZB. Development of high-viscosity, two-paste bioactive bone cements. Biomaterials. 2005;26:3713–8.CrossRefGoogle Scholar
  33. 33.
    Park J, Lakes R. Biomaterials: an introduction. New York: Springer; 2007. p. 185–8.Google Scholar
  34. 34.
    Kamitakahara M, Kawashita M, Kokubo T, Nakamura T. Effect of polyacrylic acid on the apatite formation of a bioactive ceramic in a simulated body fluid: fundamental examination of the possibility of obtaining bioactive glass-ionomer cements for orthopaedic use. Biomaterials. 2001;23:3191–6.CrossRefGoogle Scholar
  35. 35.
    Shinzato S, Nakamura T, Kokubo T, Kitamura Y. Bioactive bone cement: effect of filler size on mechanical properties and osteoconductivity. J Biomed Mater Res. 2001;56:452–8.CrossRefGoogle Scholar
  36. 36.
    Takagi S, Chow LC, Ishikawa K. Formation of hydroxyapatite in new calcium phosphate cements. Biomaterials. 1998;19:1593–9.CrossRefGoogle Scholar
  37. 37.
    Saravanapavan P, Jones JR, Pryce RS, Hench LL. Bioactivity of gel-glass powders in the CaO–SiO2 system: a comparison with ternary (CaO–P2O5–SiO2) and quaternary glasses (SiO2–CaO–P2O5–Na2O). J Biomed Mater Res A. 2003;66:110–9.CrossRefGoogle Scholar
  38. 38.
    Kokubo T, Kim HM, Kawashita M. Novel bioactive materials with different mechanical properties. Biomaterials. 2003;24:2161–75.CrossRefGoogle Scholar
  39. 39.
    Fukuda R, Yoshida Y, Nakayama Y, Okazaki M, Inoue S, Sano H, Suzuki K, Shintani H, Meerbeek BV. Bonding efficacy of polyalkenoic acids to hydroxyapatite enamel and dentin. Biomaterials. 2003;24:1861–7.CrossRefGoogle Scholar
  40. 40.
    Matsumura K, Hayami T, Hyon SH, Tsutsumi S. Control of proliferation and differentiation of osteoblasts on apatite-coated poly(vinyl alcohol) hydrogel as an artificial articular cartilage material. J Biomed Mater Res A. 2010;15:1225–32.Google Scholar
  41. 41.
    Kim SS, Sun Park M, Jeon O, Yong Choi C, Kim BS. Poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds for bone tissue engineering. Biomaterials. 2006;27:1399–409.CrossRefGoogle Scholar
  42. 42.
    Cerroni L, Filocamo R, Fabbri M, Piconi C, Caropreso S, Condò SG. Growth of osteoblast-like cells on porous hydroxyapatite ceramics: an in vitro study. Biomol Eng. 2002;19:119–24.CrossRefGoogle Scholar
  43. 43.
    Shu R, McMullen R, Baumann MJ, McCabe LR. Hydroxyapatite accelerates differentiation and suppresses growth of MC3T3-E1 osteoblasts. J Biomed Mater Res A. 2003;15(67):1196–204.CrossRefGoogle Scholar
  44. 44.
    Nakamura M, Nagai A, Tanaka Y, Sekijima Y, Yamashita K. Polarized hydroxyapatite promotes spread and motility of osteoblastic cells. J Biomed Mater Res A. 2010;92:783–90.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Materials and Energy Research CenterTehranIran

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