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Novel fatty acid gentamicin salts as slow-release drug carrier systems for anti-infective protection of vascular biomaterials

  • A. ObermeierEmail author
  • F. D. Matl
  • J. Schwabe
  • A. Zimmermann
  • K. D. Kühn
  • S. Lakemeier
  • R. v. Eisenhart-Rothe
  • A. Stemberger
  • R. Burgkart
Article

Abstract

Infections of vascular prostheses are still a major risk in surgery. The current work presents an in vitro evaluation of novel slow release antibiotic coatings based on new gentamicin fatty acid salts for polytetrafluoroethylene grafts. These grafts were coated with gentamicin sodium dodecyl sulfate, gentamicin laurate and gentamicin palmitate. Drug release kinetics, anti-infective characteristics, biocompatibility and haemocompatibility of developed coatings were compared to commercially available gelatin sealed PTFE grafts (SEALPTFE™) and knitted silver coated Dacron® grafts (InterGard®). Each gentamicin fatty acid coating showed a continuous drug release in the first eight hours followed by a low continuous release. Grafts coated with gentamicin fatty acids reduced bacterial growth even beyond pathologically relevant high concentrations. Cytotoxicity levels depending on drug formulation bringing up gentamicin palmitate as the most promising biocompatible coating. Thrombelastography studies, ELISA assays and an amidolytic substrate assay confirmed haemocompatibility of developed gentamicin fatty acid coatings comparable to commercially available grafts.

Keywords

Drug Release Gentamicin Vascular Graft Vascular Prosthesis PTFE Graft 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was partially financial supported by the Heraeus Medical GmbH (Werheim, Germany). We would like to thank V. Vatou (Institute for Medical Microbiology and Hygiene at the Klinikum rechts der Isar, TU München) for her great assistance in microbial testing. Furthermore, we acknowledge T. C. Hohnschildt for her contribution to this publication.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • A. Obermeier
    • 1
    Email author
  • F. D. Matl
    • 1
  • J. Schwabe
    • 2
  • A. Zimmermann
    • 3
  • K. D. Kühn
    • 4
  • S. Lakemeier
    • 5
  • R. v. Eisenhart-Rothe
    • 1
  • A. Stemberger
    • 1
  • R. Burgkart
    • 1
  1. 1.Klinik für Orthopädie und Sportorthopädie, Klinikum r.d. IsarTechnische Universität München, Muskuloskelettale ForschungMunichGermany
  2. 2.Alfried Krupp Krankenhaus Essen-SteeleKlinik für Unfallchirurgie und OrthopädieEssenGermany
  3. 3.Klinik für Gefäßchirurgie, Klinikum r.d. IsarTechnische Universität MünchenMunichGermany
  4. 4.Universitätsklinik für OrthopaedieMedizinische Universität GrazGrazAustria
  5. 5.Universitätsklinikum Giessen und Marburg GmbHKlinik für Orthopädie und RheumatologieMarburgGermany

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