Molecularly imprinted nanoparticles prepared by miniemulsion polymerization as selective receptors and new carriers for the sustained release of carbamazepine

  • Mehdi Esfandyari-Manesh
  • Mehran Javanbakht
  • Rassoul Dinarvand
  • Fatemeh Atyabi


Water-compatible imprinted nanoparticles were prepared for carbamazepine as a template and used for the selective extraction and controlled release of carbamazepine. Assay materials and drug delivery carriers were typically used in aqueous environments, so it is generally preferable to prepare solvent-free molecularly imprinted nanoparticles in water using the miniemulsion polymerization method. The present work investigates a bio-analytical strategy generically applicable to imprinted materials for molecular recognition studies, including equilibrium and non-equilibrium binding, and release experiments, increasing the knowledge of the molecular interactions between the template molecules and imprinted nanoparticles. The results showed that the imprinted nanoparticles exhibited a higher binding level and slower release rate than non-imprinted nanoparticles. The selectivity of imprinted nanoparticles for carbamazepine studied in comparison with an analogue compound, oxcarbazepine, the main metabolite of carbamazepine. The recovery and selectivity of carbamazepine in human serum was determined to be 100%, 1.7 times that of oxcarbazepine. The results indicated that carbamazepine-imprinted nanoparticles are appropriate for serum level determination of the drug in therapeutic range. The template to functional monomer ratio as a key factor controlling the recognition and release kinetic mechanism of imprinted nanoparticles is discussed. The imprinted nanoparticles prepared at the appropriate template to functional monomer mole ratio (2:8) exhibited the best drug affinity (5.1 times higher) and a slower drug release rate due to the interaction of carbamazepine with the imprinted cavities within the nanoparticles. Loaded imprinted nanoparticles as drug reservoirs were able to prolong carbamazepine release, in 1% wt sodium dodecyl sulfate aqueous solution, for more than 8 days.





Molecularly imprinted polymer


Non-molecularly imprinted polymer




Methacrylic acid


Ethylene glycol dimethacrylate




Sodium dodecyl sulfate


Differential scanning calorimetry


Scanning electron microscopy

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mehdi Esfandyari-Manesh
    • 1
  • Mehran Javanbakht
    • 1
    • 2
  • Rassoul Dinarvand
    • 3
  • Fatemeh Atyabi
    • 3
  1. 1.Department of ChemistryAmirkabir University of TechnologyTehranIran
  2. 2.Nano Science and Technology Research CenterAmirkabir University of TechnologyTehranIran
  3. 3.Nanotechnology Research Center Faculty of PharmacyTehran University of Medical SciencesTehranIran

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