Assay of in vitro osteoclast activity on dentine, and synthetic calcium phosphate bone substitutes
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Resorption of synthetic bone substitute materials is essential for the integration of these materials into the natural bone remodeling process. Osteoclast behavior in the presence of calcium phosphate bioceramics (CaPB) is partially understood, and a better understanding of the underlying mechanisms is expected to facilitate the development of new synthetic bone substitutes to improve bone regeneration. In the present study, our aim was to investigate osteoclastic resorption of various synthetic CaPB. We used neonatal total rabbit bone cells to generate osteoclasts. Osteoclast-generated resorption on dentine and multiple CaPB was investigated by quantifying the surface resorbed and measuring tartrate resistant acid phosphatase (TRAP) enzyme activity. In this study, we observed that osteoclastic cells responded in a different way to each substrate. Both dentine and CaPB were resorbed but the quantitative results for the surface resorbed and TRAP activity showed a specific response to each substrate and that increased mineral density seemed to inhibit osteoclast activity.
KeywordsBone Substitute Tartrate Resistant Acid Phosphatase Biphasic Calcium Phosphate Resorption Lacuna Bone Substitute Material
This work was supported by a grant from INSERM and the Région des pays de la Loire. We would like to thank Dr. Laetitia Obadia for kindly providing the biomaterial pellets.
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