Assay of in vitro osteoclast activity on dentine, and synthetic calcium phosphate bone substitutes
- 446 Downloads
Resorption of synthetic bone substitute materials is essential for the integration of these materials into the natural bone remodeling process. Osteoclast behavior in the presence of calcium phosphate bioceramics (CaPB) is partially understood, and a better understanding of the underlying mechanisms is expected to facilitate the development of new synthetic bone substitutes to improve bone regeneration. In the present study, our aim was to investigate osteoclastic resorption of various synthetic CaPB. We used neonatal total rabbit bone cells to generate osteoclasts. Osteoclast-generated resorption on dentine and multiple CaPB was investigated by quantifying the surface resorbed and measuring tartrate resistant acid phosphatase (TRAP) enzyme activity. In this study, we observed that osteoclastic cells responded in a different way to each substrate. Both dentine and CaPB were resorbed but the quantitative results for the surface resorbed and TRAP activity showed a specific response to each substrate and that increased mineral density seemed to inhibit osteoclast activity.
KeywordsBone Substitute Tartrate Resistant Acid Phosphatase Biphasic Calcium Phosphate Resorption Lacuna Bone Substitute Material
This work was supported by a grant from INSERM and the Région des pays de la Loire. We would like to thank Dr. Laetitia Obadia for kindly providing the biomaterial pellets.
- 2.Roodman GD, Windle JJ. Paget disease of bone. J Clin Invest. 2005;115(2):200–8.Google Scholar
- 4.Blair HC, Athanasou NA. Recent advances in osteoclast biology and pathological bone resorption. Histol Histopathol. 2004;19(1):189–99.Google Scholar
- 6.Soueidan A, Gan OI, Gouin F, Godard A, Heymann D, Jacques Y, Daculsi G. Culturing of cells from giant cell tumour of bone on natural and synthetic calcified substrata: the effect of leukaemia inhibitory factor and vitamin D3 on the resorbing activity of osteoclast-like cells. Virchows Arch. 1995;426(5):469–77.CrossRefGoogle Scholar
- 11.Chambers TJ, Thomson BM, Fuller K. Effect of substrate composition on bone resorption by rabbit osteoclasts. J Cell Sci. 1984;70:61–71.Google Scholar
- 12.Obadia L, Rouillon T, Bujoli B, Daculsi G, Bouler JM. Calcium-deficient apatite synthesized by ammonia hydrolysis of dicalcium phosphate dihydrate: influence of temperature, time, and pressure. J Biomed Mater Res B. 2007;80(1):32–42.Google Scholar
- 17.Fuller K, Ross JL, Szewczyk KA, Moss R, Chambers TJ. Bone is not essential for osteoclast activation. PLoS One. 2010;17(5):9.Google Scholar
- 20.Grimandi G, Soueidan A, Anjrini AA, Badran Z, Pilet P, Daculsi G, Faucheux C, Bouler JM, Guicheux J. Quantitative and reliable in vitro method combining scanning electron microscopy and image analysis for the screening of osteotropic modulators. Microsc Res Tech. 2006;69(8):606–12.CrossRefGoogle Scholar
- 22.Winkler T, Hoenig E, Huber G, Janssen R, Fritsch D, Gildenhaar R, Berger G, Morlock MM, Schilling AF. Osteoclastic bioresorption of biomaterials: two- and three-dimensional imaging and quantification. Int J Artif Organs. 2010;33(4):198–203.Google Scholar
- 28.Halleen JM. Tartrate-resistant acid phosphatase 5B is a specific and sensitive marker of bone resorption. Anticancer Res. 2003;23(2A):1027–9.Google Scholar
- 31.Halleen JM, Raisanen S, Salo JJ, Reddy SV, Roodman GD, Hentunen TA, Lehenkari PP, Kaija H, Vihko P, Vaananen HK. Intracellular fragmentation of bone resorption products by reactive oxygen species generated by osteoclastic tartrate-resistant acid phosphatase. J Biol Chem. 1999;274(33):22907–10.CrossRefGoogle Scholar