Novel biodegradable, biomimetic and functionalised polymer scaffolds to prevent expansion of post-infarct left ventricular remodelling
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Over the past decade, a large number of strategies and technologies have been developed to reduce heart failure progression. Among these, cardiac tissue engineering is one of the most promising. Aim of this study is to develop a 3D scaffold to treat cardiac failure. A new three-block copolymer, obtained from δ-valerolactone and polyoxyethylene, was synthesised under high vacuum without catalyst. Copolymer/gelatine blends were microfabricated to obtain a ECM-like geometry. Structures were studied under morphological, mechanical, degradation and biological aspects. To prevent left ventricular remodelling, constructs were biofunctionalises with molecularly imprinted nanoparticles towards the matrix metalloproteinase MMP-9. Results showed that materials are able to reproduce the ECM structure with high resolution, mechanical properties were in the order of MPa similar to those of the native myocardium and cell viability was verified. Nanoparticles showed the capability to rebind MMP-9 (specific rebinding 18.67) and to be permanently immobilised on the scaffold surface.
KeywordsLeft Ventricular Assistance Device Ventricular Assistance Device Soft Lithography Molecular Imprint Cardiac Tissue Engineering
Authors would acknowledge Dr. F. Boccafoschi (University of Eastern Piedmont “Amedeo Avogadro”, Novara, Italy) for cytotoxicity tests and Dr. T. Prescimone (Institute of Clinical Physiology, National Research Council, Pisa, Italy) for zymography analysis. This work was financially supported by Italian Ministry of University and Research PRIN-2008 grant (Bioartificial stem cell niches for cardiac tissue engineering, 2010–2012).
- 5.Giusti P, Lazzeri L, Lelli L. Bioartificial polymeric materials: a new method to design biomaterials by using both biological and synthetic polymers. Trends Polym Sci. 1993;1:261–7.Google Scholar
- 14.Wang PY, Yu J, Lin JH, Tsai WB. Modulation of alignment, elongation and contraction of cardiomyocytes through a combination of nanotopography and rigidity of substrates. Acta Biomater. 2011. doi: 10.1016/j.actbio.2011.05.021.
- 17.Rosellini E, Vozzi G, Barbani N, Giusti P, Cristallini C. Three-dimensional microfabricated scaffolds with cardiac extracellular matrix-like architecture. Int J Artif Organs. 2010;33:885–94.Google Scholar
- 19.Khademhosseini A, Langer R. Nanobiotechnology for drug delivery and tissue engineering. Chem Eng Prog. 2006;102:38–42.Google Scholar
- 22.Shea KJ. Molecular imprinting of synthetic network polymers: the de novo synthesis of macromolecular binding and catalytic sites. Trends Polym Sci. 1994;2:166–73.Google Scholar
- 23.Steinke J, Sherrington D, Dunkin I. Imprinting of synthetic polymers using molecular templates. Adv Polym Sci. 1995;123:80–125.Google Scholar
- 34.Sbarbati-Del Guerra R, Cascone MG, Tricoli M, Cerrai P. In vitro validation of poly(ester–ether–ester) block copolymers as biomaterials. Altern Lab Anim. 1993;21:97–101.Google Scholar
- 41.Guerra GD, Cristallini C, Barbani N, Gagliardi M. Bioresorbable microspheres as devices for the controlled release of paclitaxel. Int J Biol Biomed Eng. 2011;5:121–8.Google Scholar