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Compatibility of different polymers for cord blood-derived hematopoietic progenitor cells

  • Mónica Ventura Ferreira
  • Norina Labude
  • Daniela Piroth
  • Willi Jahnen-Dechent
  • Ruth Knüchel
  • Thomas Hieronymus
  • Martin Zenke
  • Sabine Neuss
Article

Abstract

The low yield of hematopoietic progenitor cells (HPC) present in cord blood grafts limits their application in clinics. A reliable strategy for ex vivo expansion of functional HPC is a present goal in regenerative medicine. Here we evaluate the capacity of several two-dimensional polymers to support HPC proliferation. Basic compatibility was tested by measuring cell viability, cytotoxicity and apoptosis of CD34+ progenitors that were short and long-term exposed to sixteen bio and synthetic polymers. Resomer® RG503, PCL and Fibrin might be good alternatives to tissue culture plastic for culture of CB-derived CD34+ progenitors. Further, these polymers will be produced in three-dimensional structures and tested for their cytocompatibility.

Keywords

Hyaluronic Acid PDMS PLLA Hematopoietic Progenitor Cell International Standardization Organization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was financially supported by the Federal Ministry of Education and Research (BMBF), Germany (Grant-Number: 01GN0931). We thank the Department of Gynecology and Obstetrics from the University Hospital Aachen for the cooperation providing the cord blood samples. We thank the DWI e.V. and Institute of Technical and Macromolecular Chemistry, from the RWTH Aachen University, for the cooperation related to polymer production.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Mónica Ventura Ferreira
    • 1
  • Norina Labude
    • 1
  • Daniela Piroth
    • 2
  • Willi Jahnen-Dechent
    • 3
    • 4
  • Ruth Knüchel
    • 1
  • Thomas Hieronymus
    • 3
    • 5
  • Martin Zenke
    • 3
    • 5
  • Sabine Neuss
    • 1
    • 3
    • 4
  1. 1.Institute of PathologyRWTH Aachen UniversityAachenGermany
  2. 2.Department for GynecologyRWTH Aachen UniversityAachenGermany
  3. 3.Helmholtz Institute for Biomedical EngineeringRWTH Aachen UniversityAachenGermany
  4. 4.Institute for Biomedical Engineering, Biointerface GroupRWTH Aachen UniversityAachenGermany
  5. 5.Department of Cell Biology, Institute for Biomedical EngineeringRWTH Aachen UniversityAachenGermany

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