Compatibility of different polymers for cord blood-derived hematopoietic progenitor cells
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The low yield of hematopoietic progenitor cells (HPC) present in cord blood grafts limits their application in clinics. A reliable strategy for ex vivo expansion of functional HPC is a present goal in regenerative medicine. Here we evaluate the capacity of several two-dimensional polymers to support HPC proliferation. Basic compatibility was tested by measuring cell viability, cytotoxicity and apoptosis of CD34+ progenitors that were short and long-term exposed to sixteen bio and synthetic polymers. Resomer® RG503, PCL and Fibrin might be good alternatives to tissue culture plastic for culture of CB-derived CD34+ progenitors. Further, these polymers will be produced in three-dimensional structures and tested for their cytocompatibility.
KeywordsHyaluronic Acid PDMS PLLA Hematopoietic Progenitor Cell International Standardization Organization
This work was financially supported by the Federal Ministry of Education and Research (BMBF), Germany (Grant-Number: 01GN0931). We thank the Department of Gynecology and Obstetrics from the University Hospital Aachen for the cooperation providing the cord blood samples. We thank the DWI e.V. and Institute of Technical and Macromolecular Chemistry, from the RWTH Aachen University, for the cooperation related to polymer production.
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