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Journal of Materials Science: Materials in Medicine

, Volume 25, Issue 12, pp 2659–2667 | Cite as

Ex vivo bio-compatibility of honey-alginate fibrous matrix for HaCaT and 3T3 with prime molecular expressions

  • Ananya Barui
  • Ritesh Khare
  • Santanu Dhara
  • Provas Banerjee
  • Jyotirmoy Chatterjee
Article

Abstract

Honey’s inherent compositional diversity, bio-compatibility and time tested therapeutic efficacy, especially in tissue repair as a topical agent, attract researchers towards harnessing its biomaterial potential particularly in developing matrix for tissue engineering applications. Hence, this study fabricates fibrous mat from optimum honey-alginate formulation and alginate solution using wet spinning technology. The physical and morphological properties of the scaffolds are assessed and finally their comparative biological performances are evaluated through in vitro studies on adherence, viability and prime molecular expression of HaCaT and 3T3 cells. The honey-alginate scaffold demonstrates better performance than that of alginate in terms of cellular adherence, viability and proper expression of cell–cell adhesion molecule (E-cadherin) and prime molecules of extra cellular matrix (Collagen I and III) by HaCaT and 3T3 respectively.

Keywords

Alginate Tissue Engineering HaCaT Cell Tissue Engineering Application Cutaneous Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

Financial support from ISIRD, SRIC, IIT Kharagpur (IIT/SRIC/ISIRD/2007-08, 02.01.2008) for this work is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.School of Medical Science & TechnologyIIT KharagpurKharagpurIndia
  2. 2.Banerjee’s Biomedical Research FoundationSainthiaIndia

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