Oxygen consumption, acidification and migration capacity of human primary osteoblasts within a three-dimensional tantalum scaffold
- 219 Downloads
A major clinical problem within synthetic, large-scaled scaffolds is the insufficient nutrient supply resulting in inhomogeneous cell proliferation and differentiation. The aim of this study was to analyse pH value, oxygen consumption and migration of human osteoblasts within a 3D tantalum scaffold, clinically used for larger bone defects. After 24 h the oxygen concentration within the scaffold decreased significantly and remained low during incubation. Monitoring of the pH value inside the tantalum scaffold showed a slightly acidification under static culture conditions. However, cell migration within the 3D scaffold was detected. Hence, in clinical application it can be assumed that porous tantalum scaffolds can be settled by osteoblasts under critical oxygen and nutrient supply. In general, monitoring of cell migration, oxygen consumption and acidification can be a suitable instrument for creating advanced 3D bone scaffolds.
KeywordsTantalum Human Osteoblast Large Bone Defect High Oxygen Consumption Trabecular Metal
The authors gratefully thank the European Union and the Ministry of Economic Affairs, Employment and Tourism of Mecklenburg-Vorpommern for financial support within the project “Tissue Regeneration”, sub-project “BONET”. We acknowledge Ms. Ricarda Niendorf for her technical support.
Conflict of interest
- 1.Hubble MJ. Bone grafts. Surg Technol Int. 2002;10:261–5.Google Scholar
- 2.Menze M. The bone graft and bone substitute market—aint nothing like the real thing? Review and analysis of trends and revenues in the bone graft market. PearlDiver Inc 2008.Google Scholar
- 3.Rueger JM. Bone substitution materials current status and prospects. Orthopade. 1998;27:72–9.Google Scholar
- 6.Grob D. Problems at the donor site in autologous bone transplantation. Unfallchirurg. 1986;89:339–45.Google Scholar
- 15.Muschler GF, Nakamoto C, Griffith LG. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg Am. 2004;86-A:1541–58.Google Scholar
- 17.Cohen R. A porous tantalum trabecular metal: basic science. Am J Orthop (Belle Mead NJ). 2002;31:216–7.Google Scholar
- 18.Christie MJ. Clinical applications of trabecular metal. Am J Orthop (Belle Mead NJ). 2002;31:219–20.Google Scholar
- 19.Welldon KJ, Atkins GJ, Howie DW, Findlay DM. Primary human osteoblasts grow into porous tantalum and maintain an osteoblastic phenotype. J Biomed Mater Res A. 2008;84:691–701.Google Scholar
- 21.Shimko DA, Shimko VF, Sander EA, Dickson KF, Nauman EA. Effect of porosity on the fluid flow characteristics and mechanical properties of tantalum scaffolds. J Biomed Mater Res B Appl Biomater. 2005;73:315–24.Google Scholar
- 27.Frick KK, Jiang L, Bushinsky DA. Acute metabolic acidosis inhibits the induction of osteoblastic egr-1 and type 1 collagen. Am J Physiol. 1997;272:C1450–6.Google Scholar