In vitro study in stimulating the secretion of angiogenic growth factors of strontium-doped calcium polyphosphate for bone tissue engineering

  • Fei Liu
  • Xu Zhang
  • Xixun Yu
  • Yuanting Xu
  • Ting Feng
  • Dawei Ren
Article
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Abstract

Angiogenesis of tissue-engineered bone remains a limited factor for the engineering of larger bone tissue constructs. Attempts to stimulate angiogenesis, using recombinant protein or gene transfer of angiogenic growth factors, have been proposed; however, these approaches have been associated with some problems regarding such as complex technique, expensive prices as well as safety problems and short half-life of angiogenic growth factors. This study was performed to determine the ability of strontium-doped calcium polyphosphate (SCPP) to induce angiogenesis via researching its effect on the mRNA expressions and protein secretion of VEGF and bFGF in/from cultured osteoblasts (ROS17/2.8 cells). We cultured osteoblasts with SCPP scaffolds containing various doses of strontium as well as calcium polyphosphate (CPP) scaffold. Through the detection of MTT and SEM, we have found that SCPP could promote cell proliferation and maintain their morphology. The results of RT–PCR and ELISA indicated that, compared with those in CPP group, the mRNA expression as well as protein levels of VEGF and bFGF in/from cultured osteoblasts were dose-dependent increasing in response to increasing strontium before reaching the peak in SCPP groups, and 8% SCPP showed the optimal promoting role. Therefore, SCPP containing proper dose of strontium could be served as a potential biomaterial with stimulating angiogenesis in bone tissue engineering and bone repair.

Keywords

Vascular Endothelial Growth Factor Strontium Strontium Ranelate Bone Tissue Engineering Angiogenic Growth Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (30870616), Basic Research Program of Sichuan Province-China (07JY029-075) and Natural Science Foundation of Jiangsu Province-China (BK2008152). We would like to thank the Analysis and Testing Center, Sichuan University (China) for their assistance of the completion of scanning electron microscopy analysis.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Fei Liu
    • 1
    • 2
  • Xu Zhang
    • 1
  • Xixun Yu
    • 1
    • 2
  • Yuanting Xu
    • 1
  • Ting Feng
    • 3
  • Dawei Ren
    • 1
  1. 1.College of Polymer Science and EngineeringSichuan UniversityChengduPeople’s Republic of China
  2. 2.Suzhou Institute of Sichuan UniversitySuzhouPeople’s Republic of China
  3. 3.West China Second University HospitalSichuan UniversityChengduPeople’s Republic of China

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