Na-doped β-tricalcium phosphate: physico-chemical and in vitro biological properties
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Synthetic calcium phosphate ceramics as β-tricalcium phosphate (Ca3(PO4)2; β-TCP) are currently successfully used in human bone surgery. The aim of this work was to evaluate the influence of the presence of sodium ion in β-TCP on its mechanical and biological properties. Five Na-doped-β-TCP [Ca10.5−x/2Na x (PO4)7, 0 ≤ x ≤ 1] microporous pellets were prepared via solid phase synthesis, and their physico-chemical data (lattice compacity, density, porosity, compressive strength, infrared spectra) denote an increase of the mechanical properties and a decrease of the solubility when the sodium content is raised. On the other hand, the in vitro study of MC3T3-E1 cell activity (morphology, MTS assay and ALP activity) shows that the incorporation of sodium does not modify the bioactivity of the β-TCP. These results strongly suggest that Na-doped-β-TCP appear to be good candidates for their use as bone substitutes.
KeywordsCompressive Strength Bone Substitute Transwell Insert Calcium Phosphate Ceramic Phosphate Buffer Salt
This work was supported by CNRS “Programme Matériaux Nouveaux, Nouvelles Fonctionnalités”, Région Pays de Loire “Programme Biomatériaux S3” and Fondation Avenir pour la Recherche Médicale appliquée, étude ET2-321. Marion Julien received a fellowship from INSERM and region des Pays de la Loire.
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