Joint immobilization inhibits spontaneous hyaline cartilage regeneration induced by a novel double-network gel implantation
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We have recently discovered that spontaneous hyaline cartilage regeneration can be induced in an osteochondral defect in the rabbit, when we implant a novel double-network (DN) gel plug at the bottom of the defect. To clarify whether joint immobilization inhibits the spontaneous hyaline cartilage regeneration, we conducted this study with 20 rabbits. At 4 or 12 weeks after surgery, the defect in the mobile knees was filled with a sufficient volume of the hyaline cartilage tissue rich in proteoglycan and type-2 collagen, while no cartilage tissues were observed in the defect in the immobilized knees. Type-2 collagen, Aggrecan, and SOX9 mRNAs were expressed only in the mobile knees at each period. This study demonstrated that joint immobilization significantly inhibits the spontaneous hyaline cartilage regeneration induced by the DN gel implantation. This fact suggested that the mechanical environment is one of the significant factors to induce this phenomenon.
KeywordsCartilage Tissue Chondrogenic Differentiation Hyaline Cartilage Osteochondral Defect Cartilage Regeneration
This work was financially supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and from Takeda Science Foundation, Japan.
Conflict of interest
The authors declared that they had no conflicts of interests in their authorship and publication of the contribution.
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