Journal of Materials Science: Materials in Medicine

, Volume 22, Issue 2, pp 279–288

Fabrication of chitosan/poly(ε-caprolactone) composite hydrogels for tissue engineering applications

  • Xia Zhong
  • Chengdong Ji
  • Andrew K. L. Chan
  • Sergei G. Kazarian
  • Andrew Ruys
  • Fariba Dehghani
Article
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Abstract

The aim of this study was to fabricate three-dimensional (3D) porous chitosan/poly(ε-caprolactone) (PCL) hydrogels with improved mechanical properties for tissue engineering applications. A modified emulsion lyophilisation technique was developed to produce 3D chitosan/PCL hydrogels. The addition of 25 and 50 wt% of PCL into chitosan substantially enhanced the compressive strength of composite hydrogel 160 and 290%, respectively, compared to pure chitosan hydrogel. The result of ATR–FTIR imaging corroborated that PCL and chitosan were well mixed and physically co-existed in the composite structures. The composite hydrogels were constructed of homogenous structure with average pore size of 59.7 ± 14 μm and finer pores with average size of 4.4 ± 2 μm on the wall of these larger pores. The SEM and confocal laser scanning microscopy images confirmed that fibroblast cells were attached and proliferated on the 3D structure of these composite hydrogels. The composite hydrogels acquired in this study possessed homogeneous porous structure with improved mechanical strength and integrity. They may have a high potential for the production of 3D hydrogels for tissue engineering applications.

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Xia Zhong
    • 1
  • Chengdong Ji
    • 1
  • Andrew K. L. Chan
    • 2
  • Sergei G. Kazarian
    • 2
  • Andrew Ruys
    • 3
  • Fariba Dehghani
    • 1
  1. 1.School of Chemical and Biomolecular EngineeringUniversity of SydneySydneyAustralia
  2. 2.Department of Chemical EngineeringImperial College LondonLondonUK
  3. 3.School of Aerospace, Mechanical and Mechatronic EngineeringUniversity of SydneySydneyAustralia

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