Sputter deposited bioceramic coatings: surface characterisation and initial protein adsorption studies using surface-MALDI-MS
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Protein adsorption onto calcium phosphate (Ca–P) bioceramics utilised in hard tissue implant applications has been highlighted as one of the key events that influences the subsequent biological response, in vivo. This work reports on the use of surface-matrix assisted laser desorption ionisation mass spectrometry (Surface-MALDI-MS) as a technique for the direct detection of foetal bovine serum (FBS) proteins adsorbed to hybrid calcium phosphate/titanium dioxide surfaces produced by a novel radio frequency (RF) magnetron sputtering method incorporating in situ annealing between 500°C and 700°C during deposition. XRD and XPS analysis indicated that the coatings produced at 700°C were hybrid in nature, with the presence of Ca–P and titanium dioxide clearly observed in the outer surface layer. In addition to this, the Ca/P ratio was seen to increase with increasing annealing temperature, with values of between 2.0 and 2.26 obtained for the 700°C samples. After exposure to FBS solution, surface-MALDI-MS indicated that there were significant differences in the protein patterns as shown by unique peaks detected at masses below 23.1 kDa for the different surfaces. These adsorbates were assigned to a combination of growth factors and lipoproteins present in serum. From the data obtained here it is evident that surface-MALDI-MS has significant utility as a tool for studying the dynamic nature of protein adsorption onto the surfaces of bioceramic coatings, which most likely plays a significant role in subsequent bioactivity of the materials.
KeywordsProtein Adsorption Hybrid Coating Titanium Layer Post Deposition Annealing ICDD File
This work was kindly supported by a RTD Networking grant from Invest Northern Ireland (RTD 375). This work was part of the Centre for Nanostructured Polymer Surfaces for Medical Applications, funded by the Danish Ministry for Science, Technology and Innovation (2002-603-4001-87). The authors would also like to thank Kratos Analytical (UK) for their assistance with the XPS analysis.
- 11.Meenan BJ, Boyd A, Leyland NS, Love E, Akay M. The influence of substrate morphology on the structure and composition of RF sputter deposited calcium phosphate thin films. Bioceramics. 1999;12:471–4.Google Scholar
- 38.Zheng X, Baker H, Hancock WS, Fawaz F, McCaman M, Pungor E Jr. Proteomic analysis for the assessment of different lots of fetal bovine serum as a raw material for cell culture. Part IV. Application of proteomics to the manufacture of biological drugs. Biotechnol Prog. 2006;22(5):1294–300.CrossRefGoogle Scholar
- 39.Kingshott P, Hoecker H. Adsorption of proteins: assessment methods. In: Somasundaran P, editor. Encyclopedia of surface and colloid science, vol 5. 2st ed. New York: Taylor and Francis; 2006. p. 669–94.Google Scholar
- 44.Konashi K, Kambara M, Noshi H, Uemura MJ. X-ray photoelectron spectroscopic (ESCA) study on the surface of hydroxyapatite. J Osaka Dent Univ. 1987;21:1–8.Google Scholar
- 53.Xu S, Long J, Sim L, Diong CH, Ostrikov K (Ken). RF plasma sputtering deposition of hydroxyapatite bioceramics: synthesis, performance, and biocompatibility. Plasma process. Polymer. 2005;2:373–90.Google Scholar
- 54.Qian WJ, Jacobs JM, Camp DG II, Monroe ME, Moore RJ, Gritsenko MA, Calvano SE, Lowry SF, Xiao W, Moldawer LL, Davis RW, Tompkins RG, Smith RD. Comparative proteome analyses of human plasma following in vivo lipopolysaccharide administration using multidimensional separations coupled with tandem mass spectrometry. Proteomics. 2005;5:572–84.CrossRefGoogle Scholar
- 55.iProClass database. http://pir.georgetown.edu/.
- 57.Yan X, Scherphof GL, Kamps JAAM. Liposome opsonization. J Lipsome Res. 2005;15:109–39.Google Scholar