Preparation of silver nanoparticles with antimicrobial activities and the researches of their biocompatibilities

  • X. L. Cao
  • C. Cheng
  • Y. L. Ma
  • C. S. Zhao


Silver nanoparticles were prepared by chemical reduction method using chitosan as stabilizer and ascorbic acid as reducing agent in this work. The silver/chitosan nanocomposites were characterized in terms of their particle sizes and morphology by using UV spectrophotometer, nano-grainsize analyzer, and transmission electron microscopy. Antibacterial activities of these nanocomposites were carried out for Staphylococcus aureus and Escherichia coli. The silver nanoparticles exhibited significantly inhibition capacity towards these bacteria. Detailed studies on the biocompatibility of the silver/chitosan nanocomposites were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell adhesion test. The results indicated that these silver/chitosan nanocomposites were benefit for the proliferation and adhesion of L-929 cells, and the biocompatibilities between the nanocomposites and the cells would become better with the culturing days. We anticipated that these silver/chitosan nanocomposites could be a promising candidate as coating material in biomedical engineering and food packing fields wherein antibacterial properties and biocompatibilities are crucial.


Chitosan Silver Nanoparticles Chitosan Solution Silver Nitrate Solution Adsorption Peak 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was financially sponsored by the National Natural Science Foundation of China (No. 50673064, 50973070), and the Sichuan Youth Science and Technology Foundation, 08ZQ026-038). We should also thank our laboratory members for their generous help.


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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.College of Polymer Science and EngineeringState Key Laboratory of Polymer Materials Engineering, Sichuan UniversityChengDuChina

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