Fiber scaffolds of polysialic acid via electrospinning for peripheral nerve regeneration
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Fiber scaffolds of bioactive polysialic acid have been prepared via electrospinning for peripheral nerve regeneration. The diameter, morphology and alignment of fibers in scaffolds were adjusted by variation of electrospinning parameters, which are decisive for the cell-scaffold interaction. Due to the high water solubility of polysialic acid (poly-α-2,8-N-acetylneuraminic acid) a photoactive derivative (poly-α-2,8-N-pentenoylneuraminic acid) was used to obtain stable fiber scaffolds in water by photochemical crosslinking. At the optimized fiber scaffolds good cell viability and directed cell proliferation along the fibers was achieved by cell tests with immortalized Schwann cells.
KeywordsFiber Diameter Electrospun Fiber Electrospinning Process Polyethylene Oxide Peripheral Nerve Regeneration
This work was supported by DFG (Deutschen Forschungsgemeinschaft) and arose within the research group (FOR 548, “Polysialic acid: Towards the evaluation of a new bio-identical scaffold material”). Helpful discussions with Stefanie Böhm and Cornelia Kasper (Institut für Technische Chemie, Universität Hannover) are grateful acknowledged.