Effects of HA released calcium ion on osteoblast differentiation



Hydroxyapatite (HA) is a widely used calcium phosphate implant substitute and has dissolution property. Although HA has been shown a beneficial effect on osteoblast differentiation, the exact mechanism is still unclear. In the present study, we proposed that Ca2+ released from HA activated the expression bone associated proteins, OPN and BSP, mediated by L-type calcium channel and calcium/calmodulin-dependent protein kinase (CaMK) 2 which resulted into improved osteoblast differentiation. Results showed that HA elevated ALP expression as well as OPN and BSP expression in MC3T3-E1 cells. The result from western blot of CaMK2α indicated that HA released Ca2+ activated CaMK2 through L-type calcium channel. Furthermore, upregulation of OPN and BSP mRNA expression was significantly inhibited when blocking both the L-type calcium channel and CaMK2. These findings suggested that HA accelerated the osteoblast differentiation by releasing Ca2+.


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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Interdisciplinary Program for BioengineeringSeoul National UniversitySeoulKorea
  2. 2.Department of Craniomaxilofacial Reconstructive ScienceSchool of DentistrySeoulKorea
  3. 3.Department of Prosthodontics and Dental Research Center, School of DentistrySeoul National UniversitySeoulRepublic of Korea

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