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Low level laser therapy does not modulate the outcomes of a highly bioactive glass–ceramic (Biosilicate®) on bone consolidation in rats

  • Poliani Oliveira
  • Daniel Araki Ribeiro
  • Elaine Favaro Pipi
  • Patricia Driusso
  • Nivaldo A. Parizotto
  • Ana Claudia Muniz Renno
Article

Abstract

The main purpose of the present work was to evaluate if low level laser therapy (LLLT) can improve the effects of novel fully-crystallized glass–ceramic (Biosilicate®) on bone consolidation in tibial defects of rats. Forty male Wistar rats with tibial bone defects were used. Animals were divided into four groups: group bone defect control (CG); group bone defect filled with Biosilicate® (BG); group bone defect filled with Biosilicate®, irradiated with LLLT, at 60 J cm−2 (BG 60) and group bone defect filled with Biosilicate®, irradiated with LLLT, at 120 J cm−2 (BG 120). A low-energy GaAlAs 830 nm, CW, 0.6 mm beam diameter, 100 W cm−2, 60 and 120 J cm² was used in this study. Laser irradiation was initiated immediately after the surgery procedure and it was performed every 48 h for 14 days. Fourteen days post-surgery, the three-point bending test revealed that the structural stiffness of the groups CG and BG was higher than the values of the groups BG60 and BG120. Morphometric analysis revealed no differences between the control group and the Biosilcate® group. Interestingly, the groups treated with Biosilicate® and laser (BG 60 and BG120) showed statistically significant lower values of newly formed bone in the area of the defect when compared to negative control (CG) and bone defect group filled with Biosilicate (CB). Our findings suggest that although Biosilicate® exerts some osteogenic activity during bone repair, laser therapy is not able to modulate this process.

Keywords

Bone Defect Bioactive Glass Bone Repair Structural Stiffness Parent Glass 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Einhorn TA. The cell and molecular biology of fracture healing. Clin Orthop Relat Res. 1998;355S:S7–21.CrossRefGoogle Scholar
  2. 2.
    Gautier E, Sommer C. Guidelines for the clinical application of the LCP. Injury. 2003;34B:63–76.CrossRefGoogle Scholar
  3. 3.
    Gauthier O, Müller R, von Stechow D, Lamy B, Weiss P, Bouler JM, et al. In vivo bone regeneration with injectable calcium phosphate biomaterial: a three-dimensional micro-computed tomographic, biomechanical and SEM study. Biomaterials. 2005;26:5444–53.CrossRefPubMedGoogle Scholar
  4. 4.
    Hench LL, Polak JM. Third-generation biomedical materials. Science. 2002;295:1014–7.CrossRefPubMedADSGoogle Scholar
  5. 5.
    Hench LL. Glass and genes: the 2001 W. E. S. Turner memorial lecture. Glass Technol. 2003;44:1–10.Google Scholar
  6. 6.
    Fundação Universidade Federal de São Carlos; Universidade de São Paulo. Zanotto ED et al. Process and compositions for preparing particulate, bioactive or resorbable biosilicates for use in the treatment of oral ailments. Int. C. C03C10/00, 20 Feb. 2004, WO2004/074199.Google Scholar
  7. 7.
    Moura J, Teixeira LN, Ravagnani C, Peitl O, Zanotto ED, Beloti MM, et al. In vitro osteogenesis on a highly bioactive glass-ceramic (Biosilicate). J Biomed Mater Res A. 2007;82:545–57.PubMedGoogle Scholar
  8. 8.
    Granito RN, Ribeiro DA, Rennó AC, Ravagnani C, Bossini PS, Peitl-Filho O, Zanotto ED, Parizotto NA, Oishi J. Effects of biosilicate and bioglass 45S5 on tibial bone consolidation on rats: a biomechanical and a histological study. J Mater Sci Mater Med. 2009 [Epub ahead of print].Google Scholar
  9. 9.
    Luger EJ, Rochkind S, Wollman Y, Kogan G, Dekel S. Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med. 1998;22:97–102.CrossRefPubMedGoogle Scholar
  10. 10.
    Ozawa Y, Shimizu N, Kariya G, Abiko Y. Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone. 1998;22:347–54.CrossRefPubMedGoogle Scholar
  11. 11.
    Stein A, Benayahu D, Maltz L, Oron U. Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg. 2005;23:161–6.CrossRefPubMedGoogle Scholar
  12. 12.
    Nicola R, Jorgetti V, Rigau J, Pacheco MTT, Reis LM, Zângaro RA. Effect of low-power GaAlAs laser (660 nm) on bone structure and cell activity: an experimental animal study. Lasers Med Sci. 2003;18:89–94.CrossRefPubMedGoogle Scholar
  13. 13.
    Pinheiro AL, Limeira Junior FA, Gerbi ME. Effect of 830-nm laser light on the repair of bone defects grafted with inorganic bovine bone and decalcified cortical osseus membrane. J Clin Laser Med Surg. 2003;21:301–6.CrossRefPubMedGoogle Scholar
  14. 14.
    Yamamoto M, Tamura K, Hiratsuka K, Abiko Y. Stimulation of MCM3 gene expression in osteoblast by low level laser irradiation. Lasers Med Sci. 2001;16:213–7.CrossRefPubMedGoogle Scholar
  15. 15.
    Garavelo-Freitas I, Baranauskas V, Joazeiro PP, Padovani CR, Dal Pai-Silva M, Cruz-Hofling MA. Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochem Photobiol B. 2003;70:81–9.CrossRefGoogle Scholar
  16. 16.
    Hamajima S, Hiratsuka K, Kiyama-Kishikawa M, Tagawa T, Kawahara M, Ohta M, et al. Effect of low-level laser irradiation on osteoglycin gene expression in osteoblasts. Lasers Med Sci. 2003;18:78–82.CrossRefPubMedGoogle Scholar
  17. 17.
    Liu X, Lyon R, Meier HT, Thometz J, Haworth ST. Effect of lower-level laser therapy on rabbit tibial fracture. Photomed Laser Surg. 2007;25:487–94.CrossRefPubMedGoogle Scholar
  18. 18.
    Matsumoto MA, Ferino RF, Monteleone GF, Ribeiro DA. Low-level laser therapy modulates cyclo-oxygenase-2 expression during bone repair in rats. Laser Med Sci. 2009;24:195–201.CrossRefGoogle Scholar
  19. 19.
    Granito RN. The effects of Biosilicate on the bone consolidation in rats. PhD thesis, Federal University of São Carlos; 2009.Google Scholar
  20. 20.
    Calixto RF, Teófilo JM, Brentegani LG, Lamano-Carvalho TL. Grafting of tooth extraction socket with inorganic bovine bone or bioactive glass particles: comparative histometric study in rats. Implant Dent. 2007;16:260–9.CrossRefPubMedGoogle Scholar
  21. 21.
    Olivera MI, Martínez MP, Conti MI, Bozzini C, Bozzini CE, Alippi RM. Permanent reduction of mandibular size and bone stiffness induced in post-weaning rats by cyclophosphamide. Arch Oral Biol. 2008;54:6–11.CrossRefPubMedGoogle Scholar
  22. 22.
    Renno ACM, McDonnel P-A, Laakso L. Effect of 830 nm laser phototherapy on osteoblasts grown in vitro on Biosilicate scaffolds. Photomed Laser Surg. 2009;12:124.Google Scholar
  23. 23.
    Pinheiro ALB, Gerbi MEM. Photoengineering of bone repair processes. Photomed Laser Surg. 2006;24:169–78.CrossRefPubMedGoogle Scholar
  24. 24.
    Obradović R, Kesic L, Mihailović D, Ignjatović N, Uskoković D. Comparative efficacy analysis of biomaterials and soft lasers in repair of bone defects. J Oral Laser Appl. 2007;7:161–6.Google Scholar
  25. 25.
    Lirani-Galväo AP, Jorgetti V, Lopes da Silva O. Comparative study of how low-level laser therapy and low-intensity pulsed ultrasound affect bone repair in rats. Photomed Laser Surg. 2006;24:735–40.CrossRefPubMedGoogle Scholar
  26. 26.
    Gerbi ME, Pinheiro ALB, Marzola C, et al. Assessment of bone repair associated with the use of organic bovine bone Gen-ox organic and membrane irradiated with 830 nm. Photomed Laser Surg. 2005;23:382–8.CrossRefPubMedGoogle Scholar
  27. 27.
    Lewandrowski KU, Lorente C, Schomacker KT, Flotte TJ, Wilkes JW, Deutsch TF. Use of the Er: YAG laser for improved plating in maxillofacial surgery: comparison of bone healing in laser and drill osteotomies. Lasers Surg Med. 1996;19:40–5.CrossRefPubMedGoogle Scholar
  28. 28.
    Romanos GE, Everts H, Nentwig GH. Effects of diode and Nd: YAG laser irradiation on titanium discs: a scanning electron microscope examination. J Periodontol. 2000;71:810–5.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Poliani Oliveira
    • 1
  • Daniel Araki Ribeiro
    • 2
  • Elaine Favaro Pipi
    • 1
  • Patricia Driusso
    • 1
  • Nivaldo A. Parizotto
    • 1
  • Ana Claudia Muniz Renno
    • 2
  1. 1.Department of PhysiotherapyFederal University of São CarlosSão CarlosBrazil
  2. 2.Department of BioscienceFederal University of São PauloSantosBrazil

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