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Osteogenic activity of human periosteal sheets cultured on salmon collagen-coated ePTFE meshes

  • Tomoyuki KawaseEmail author
  • Kazuhiro Okuda
  • Hiroyuki Kogami
  • Hitoshi Nakayama
  • Masaki Nagata
  • Hiromasa Yoshie
Article

Abstract

Our animal implantation studies have demonstrated that, after osteogenic processing, cultured human periosteal sheets form osteoid tissue ectopically without the aid of conventional scaffolding materials. To improve the osteogenic activity of these periosteal sheets, we have tested the effects of including a scaffold made of salmon collagen-coated ePTFE mesh. Periosteal sheets were produced with minimal manipulation without enzymatic digestion. Outgrown cells penetrated into the coated mesh fiber networks to form complex multicellular layers and increased expression of alkaline phosphatase activity in response to the osteoinduction. In vitro mineralization was notably enhanced in the original tissue segment regions, but numerous micro-mineral deposits were also formed on the coated-fiber networks. When implanted subcutaneously into nude mice, periosteal sheets efficiently form osteoid around the mineral deposits. These findings suggest that the intricate three-dimensional mesh composed of collagen-coated fibers substantially augmented the osteogenic activity of human periosteal sheets both in vitro and in vivo.

Keywords

Mineral Deposit Osteogenic Induction Osteogenic Activity Mesh Fiber Periosteal Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors wish to acknowledge Dr. Douglas M. Burns and Dr. Makoto Kodama (Vecell Inc.) for proofreading and editorial assistance and for providing Vecell 3D-insert®, respectively. This project was funded through support by Grants-in-Aid for Scientific Research from the Ministry of Education, Sports, Science, and Technology, Japan and Japan Society for the Promotion of Science (Contact grant numbers: #17390558, #19592140, #20500406), by Health and Labour Sciences Research Grants, H20-saisei-ippan-006, and by Research for Promoting Technological Seeds (2007, 2008, 2009) and Practical Application Research from Japan Science and Technology Agency.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Tomoyuki Kawase
    • 1
    Email author
  • Kazuhiro Okuda
    • 2
  • Hiroyuki Kogami
    • 1
  • Hitoshi Nakayama
    • 1
    • 3
  • Masaki Nagata
    • 4
  • Hiromasa Yoshie
    • 2
  1. 1.Division of Oral Bioengineering, Department of Tissue Regeneration and Reconstitution, Institute of Medicine and DentistryNiigata UniversityNiigataJapan
  2. 2.Division of Periodontology, Department of Oral Biological Science, Institute of Medicine and DentistryNiigata UniversityNiigataJapan
  3. 3.Japan Science and Technology Agency Innovation Satellite NiigataNagaokaJapan
  4. 4.Division of Oral and Maxillofacial Surgery, Department of Oral Health Science, Institute of Medicine and DentistryNiigata UniversityNiigataJapan

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