Influence of fabrication parameters in cellular microarrays for stem cell studies
- 153 Downloads
Lately there has been an increasing interest in the development of tools that enable the high throughput analysis of combinations of surface-immobilized signaling factors and which examine their effect on stem cell biology and differentiation. These surface-immobilized factors function as artificial microenvironments that can be ordered in a microarray format. These microarrays could be useful for applications such as the study of stem cell biology to get a deeper understanding of their differentiation process. Here, the evaluation of several key process parameters affecting the cellular microarray fabrication is reported in terms of its effects on the mesenchymal stem cell culture time on these microarrays. Substrate and protein solution requirements, passivation strategies and cell culture conditions are investigated. The results described in this article serve as a basis for the future development of cellular microarrays aiming to provide a deeper understanding of the stem cell differentiation process.
KeywordsPMMA Protein Spot Cell Seeding Microcontact Printing PMMA Substrate
S. A. Rodríguez-Seguí and E. Martínez acknowledge funding of the Spanish Ministry of Education through FPU and Ramón y Cajal Grants, respectively. M. Funes and Dr. C. A. Mills (Barcelona Science Park) are gratefully acknowledged for helping with the cell cultures and for comments with respect to the article. This paper and the work it concerns were generated in the context of the CellPROM project, funded by the European Community as Contract No. NMP4-CT-2004-500039 and it reflects only the authors’ views.
- 36.Doyle A, Mather JP. Chapter 3. In: Doyle A, Griffiths JB, editors. Cell and tissue culture: laboratory procedures in biotechnology. New York: Wiley; 1998. p. 83–91.Google Scholar