Hydrogel-magnetic nanoparticles with immobilized l-asparaginase for biomedical applications

  • Eugenia Teodor
  • Simona-Carmen Litescu
  • Veronica Lazar
  • Raluca Somoghi
Article
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Abstract

The association of magnetic nanoparticles, which could be controlled by a magnetic field and have dimensions which facilitate their penetration in cells/tissues, with hydrogel type biopolymeric shells confer them compatibility and the capacity to retain and deliver bioactive substances. The main objective of this work is the development of a new system based on a biocompatible polymer with organic–inorganic structure capable of vectoring support for biologic active agents (l-asparaginase, e.g.). Characterization of size and morphology of the hydrogel-magnetic nanoparticles with entrapped l-asparaginase was made using Dynamic Light Scattering method, Transmission Electron Microscopy and Confocal Microscopy. The structure of magnetic nanoparticles coated with hydrogel was characterized by Fourier Transformed Infrared Spectroscopy. The cytotoxicity of nanoparticles was evaluated and also the interactions with microorganisms. We obtained hydrogel-magnetic nanoparticles with l-asparaginase entrapped, with sizes below 30 nm in dried stage, capable to penetrate the cells and tissues.

Keywords

Chitosan Hyaluronic Acid Magnetic Nanoparticles Vero Cell Magnetic Nanostructures 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

Work supported by the National Research and Development Agency of Romania, the Program of Excellence in Research (No. 129/2006-CEEX). Kindly thanks to Dr. Cristian Panaiotu (Bucharest University) for magnetic susceptibility studies.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Eugenia Teodor
    • 1
  • Simona-Carmen Litescu
    • 1
  • Veronica Lazar
    • 2
  • Raluca Somoghi
    • 3
  1. 1.National Institute for Biological Sciences, Centre of BioanalysisBucharestRomania
  2. 2.Bucharest UniversityBucharestRomania
  3. 3.National Institute for Chemistry & PetrochemistryBucharestRomania

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