Proliferation and function of MC3T3-E1 cells on freeze-cast hydroxyapatite scaffolds with oriented pore architectures
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Previous work by the authors showed that hydroxyapatite (HA) scaffolds with different types of oriented microstructures and a unique ‘elastic–plastic’ mechanical response could be prepared by unidirectional freezing of suspensions. The objective of the present work was to evaluate the in vitro cellular response to these freeze-cast HA scaffolds. Unidirectional scaffolds with approximately the same porosity (65–70%) but different pore architectures, described as ‘lamellar’ (pore width = 25 ± 5 μm) and ‘cellular’ (pore diameter = 100 ± 10 μm), were evaluated. Whereas both groups of scaffolds showed excellent ability to support the proliferation of MC3T3-E1 pre-osteoblastic cells on their surfaces, scaffolds with the cellular-type microstructure showed far better ability to support cell proliferation into the pores and cell function. These results indicate that freeze-cast HA scaffolds with the cellular-type microstructure have better potential for bone repair applications.
KeywordsAlkaline Phosphatase Activity Aqueous Suspension Bioactive Glass Pore Width Purple Formazan
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