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Proliferation and function of MC3T3-E1 cells on freeze-cast hydroxyapatite scaffolds with oriented pore architectures

  • Qiang Fu
  • Mohamed N. Rahaman
  • B. Sonny Bal
  • Roger F. Brown
Article

Abstract

Previous work by the authors showed that hydroxyapatite (HA) scaffolds with different types of oriented microstructures and a unique ‘elastic–plastic’ mechanical response could be prepared by unidirectional freezing of suspensions. The objective of the present work was to evaluate the in vitro cellular response to these freeze-cast HA scaffolds. Unidirectional scaffolds with approximately the same porosity (65–70%) but different pore architectures, described as ‘lamellar’ (pore width = 25 ± 5 μm) and ‘cellular’ (pore diameter = 100 ± 10 μm), were evaluated. Whereas both groups of scaffolds showed excellent ability to support the proliferation of MC3T3-E1 pre-osteoblastic cells on their surfaces, scaffolds with the cellular-type microstructure showed far better ability to support cell proliferation into the pores and cell function. These results indicate that freeze-cast HA scaffolds with the cellular-type microstructure have better potential for bone repair applications.

Keywords

Alkaline Phosphatase Activity Aqueous Suspension Bioactive Glass Pore Width Purple Formazan 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Qiang Fu
    • 1
    • 2
  • Mohamed N. Rahaman
    • 1
    • 2
  • B. Sonny Bal
    • 3
  • Roger F. Brown
    • 2
    • 4
  1. 1.Department of Materials Science and EngineeringMissouri University of Science and TechnologyRollaUSA
  2. 2.Center for Bone and Tissue Repair and RegenerationMissouri University of Science and TechnologyRollaUSA
  3. 3.Department of Orthopaedic SurgeryUniversity of Missouri-ColumbiaColumbiaUSA
  4. 4.Department of Biological SciencesMissouri University of Science and TechnologyRollaUSA

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