Quantification of bone mass gain in response to the application of biphasic bioceramics and platelet concentrate in critical-size bone defects

  • Sonja Ellen Lobo
  • Francisco Henrique Lanna Wykrota
  • Ana Carolina Marques Barbosa Oliveira
  • Irina Kerkis
  • Germán Bohorquez Mahecha
  • Humberto José Alves
Article

Abstract

Biphasic bioceramics have been widely indicated for bone reconstruction; however, the real gain in bone mass due to the presence of such biomaterials has not been established yet nor the advantages of its association with platelet concentrate. This study aims at quantifying the volume of bone matrix, osteoblasts, osteocytes, blood vessels and adipose tissue after the application of a biphasic bioceramics composed of 65% hydroxyapatite and 35% β-tricalcium phosphate. Critical-size bone defects were produced in rabbit femora and reconstructed with bioceramics only, with bioceramics combined with platelet concentrate, with platelet concentrate alone, and with no treatment (blood clot). The quantitative evaluation was performed on histological sections using histomorphometry. Our data provide original evidence that consolidates the indication of bioceramics for clinical bone loss reconstruction. The application of biphasic bioceramics alone led to major bone mass gain and was followed by its association with platelet concentrate. On the other hand, platelet concentrate can contribute to the augmentation and maintenance of the adipose tissue, representing a new field for future applications in plastic surgery.

Notes

Acknowledgment

This work was funded by EINCO Biomaterial Ltda (Belo Horizonte, Minas Gerais, Brazil).

References

  1. 1.
    R.W. Bucholz, A. Carlton, R.E. Holmes, Orthop. Clin. North Am. 18, 323 (1987)PubMedGoogle Scholar
  2. 2.
    F. Causa, P.A. Netti, L. Ambrosio, Biomaterials 28, 5093 (2007)PubMedCrossRefGoogle Scholar
  3. 3.
    B.R. Constantz, I.C. Ison, M.T. Fulmer, R.D. Poser, S.T. Smith, M. Vanwagoner, J. Ross, S.A. Goldstein, J.B. Jupiter, D.I. Rosenthal, Science 267, 1796 (1995)PubMedCrossRefADSGoogle Scholar
  4. 4.
    T.J. Gao, T.S. Lindholm, B. Kommonen, P. Ragni, A. Paronzini, T.C. Lindholm, Biomaterials 16, 1175 (1995)PubMedCrossRefGoogle Scholar
  5. 5.
    S. Govindaraj, P.D. Costantino, C.D. Friedman, Facial Plast. Surg. 15, 73 (1999)PubMedCrossRefGoogle Scholar
  6. 6.
    D. Le Nihouannen, A. Saffarzadeh, O. Gauthier, F. Moreau, P. Pilet, R. Spaethe, P. Layrolle, G. Daculsi, J. Mater. Sci.: Mater. Med. 19, 667 (2008)CrossRefGoogle Scholar
  7. 7.
    M. Mastrogiacomo, A. Corsi, E. Francioso, M. Di Comite, F. Monetti, S. Scaglione et al., Tissue Eng. 5, 1261 (2006)CrossRefGoogle Scholar
  8. 8.
    K. Nagahara, M. Isogai, K. Shibata, M.A. Meenaghan, Int. J. Oral Maxillofac. Implants 7, 72 (1992)PubMedGoogle Scholar
  9. 9.
    S. Sato, T. Koshino, T. Saito, Biomaterials 19, 1895 (1998)PubMedCrossRefGoogle Scholar
  10. 10.
    M. Sous, R. Bareille, F. Rouais, D. Clément, J. Amédée, B. Dupuy et al., Biomaterials 19, 2147 (1998)PubMedCrossRefGoogle Scholar
  11. 11.
    D.C. Tancred, A.J. Mccormack, A.J. Carr, Biomaterials 19, 2303 (1998)PubMedCrossRefGoogle Scholar
  12. 12.
    Z. Yang, H. Yuan, W. Tong, P. Zou, W. Chen, X. Zhang, Biomaterials 17, 2131 (1996)PubMedCrossRefGoogle Scholar
  13. 13.
    H. Yuan, K. Kurashina, J.D. De Bruijn, Y. Li, K. De Groot, X. Zhang, Biomaterials 20, 1799 (1999)PubMedCrossRefGoogle Scholar
  14. 14.
    W.J. Habraken, J.G. Wolke, J.A. Jansen, Adv. Drug Deliv. Rev. 59, 234 (2007)PubMedCrossRefGoogle Scholar
  15. 15.
    Y. Khan, M.J. Yaszemski, A.G. Mikos, C.T. Laurencin, J. Bone Joint Surg. Am. 90, 36 (2008)PubMedCrossRefGoogle Scholar
  16. 16.
    U. Ripamonti, J. Crooks, D.C. Rueger, Plast. Reconstr. Surg. 107, 977 (2001)PubMedCrossRefGoogle Scholar
  17. 17.
    S. Teixeira, M.P. Ferraz, F.J. Monteiro, J. Mater. Sci.: Mater. Med. 19, 855 (2008)CrossRefGoogle Scholar
  18. 18.
    R.Z. Legeros, S. Lin, R. Rohanizadeh, D. Mijaes, J.P. Legeros, J. Mater. Sci. Mat. Med. 14, 201 (2003)CrossRefGoogle Scholar
  19. 19.
    G. Daculsi, O. Laboux, O. Malard, P. Weiss, J. Mater. Sci. Mat. Med. 14, 195 (2003)CrossRefGoogle Scholar
  20. 20.
    H. Cerruti Filho, I. Kerkis, A. Kerkis, N.H. Tatsui, A. Da Costa Neves, D.F. Bueno et al., Artif. Organs 31, 268 (2007)CrossRefGoogle Scholar
  21. 21.
    T. Ilgenli, N. Dündar, B.I. Kal, Clin. Oral Investig. 11, 51 (2007)PubMedCrossRefGoogle Scholar
  22. 22.
    B. Klongnoi, S. Rupprecht, P. Kessker, M. Thorwarth, J. Wiltfang, K.A. Schlegel, Clin. Oral Implants Res. 17, 312 (2006)PubMedCrossRefGoogle Scholar
  23. 23.
    R.E. Marx, E.R. Carlson, R.M. Eichstaedt, S.R. Schimmele, J.E. Strauss, K.R. Georgeff, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 85, 638 (1998)PubMedCrossRefGoogle Scholar
  24. 24.
    R.E. Marx, Implant Dent. 10, 225 (2001)PubMedMathSciNetGoogle Scholar
  25. 25.
    A.S. Plachokova, J. Van Den Dolder, P.J. Stoelinga, J.A. Jansen, Clin. Oral Implants Res. 17, 305 (2006)PubMedCrossRefGoogle Scholar
  26. 26.
    D. Sonnleitner, P. Huemer, D.Y. Sullivan, Int. J. Oral Maxillofac. Implants 15, 879 (2000)PubMedGoogle Scholar
  27. 27.
    B. Azzena, F. Mazzoleni, G. Abatangelo, B. Zavan, V. Vindigni, Aesth. Plast. Surg. 32, 155 (2008)CrossRefGoogle Scholar
  28. 28.
    T.B. Jensen, O. Rahbek, S. Overgaard, K. Soballe, Int. Orthop. 29, 67 (2005)PubMedCrossRefGoogle Scholar
  29. 29.
    H.D. Kim, R.F. Valentini, Biomaterials 18, 1175 (1997)PubMedCrossRefGoogle Scholar
  30. 30.
    Y. Ogino, Y. Ayukawa, T. Kukita, K. Koyano, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 101, 724 (2006)PubMedCrossRefGoogle Scholar
  31. 31.
    A. Tomoyasu, K. Higashio, K. Kanomata, M. Goto, K. Kodaira, H. Serizawa et al., Biochem. Biophys. Res. Commun. 361, 62 (2007)PubMedCrossRefGoogle Scholar
  32. 32.
    B.L. Eppley, W.S. Pietrzak, M. Blanton, Plast. Reconstr. Surg. 118, 147e (2006)PubMedCrossRefGoogle Scholar
  33. 33.
    L.L. Wykrota, F.H.L. Wykrota, C.A. Garrido in Bone Engineering, ed. by J.E. Davies (Em2 Inc., Toronto, 2000) p. 516Google Scholar
  34. 34.
    H. Elias, A. Henning, D.E. Schwartz, Stereology: applications to biomedical research. Physiol. Rev. 51, 158–200 (1971)PubMedGoogle Scholar
  35. 35.
    R.R. Recker, Bone histomorphometry: techniques and interpretation, 1st edn. (CRC Press, Boca Raton, 1983)Google Scholar
  36. 36.
    L. Geris, A. Gerisch, J.V. Sloten, R. Weiner, H.V. Oosterwyck, J. Theor. Biol. 251, 137 (2008)PubMedCrossRefGoogle Scholar
  37. 37.
    J. Van Den Dolder, R. Mooren, A.P.G. Vloon, P.J. Stoelinga, J.A. Jansen, Tissue Eng. 12, 3067 (2006)PubMedCrossRefGoogle Scholar
  38. 38.
    D. Le Nihouannen, L.L. Le Guehennec, T. Rouillon, P. Pilet, M. Bilban, P. Layrolle et al., Biomaterials 27, 2716 (2006)PubMedCrossRefGoogle Scholar
  39. 39.
    C. Borselli, O. Oliviero, S. Battista, L. Ambrosio, P.A. Netti, J. Biomed. Mat. Res. A 80, 297 (2007)CrossRefGoogle Scholar
  40. 40.
    M. Mastrogiacomo, S. Scaglione, R. Martinetti, L. Dolcini, F. Beltrame, R. Cancedda et al., Biomaterials 27, 3230 (2006)PubMedCrossRefGoogle Scholar
  41. 41.
    D.C. Teixeira, Y. Nemelivsky, C. Karkia, R.Z. Legeros, Tissue Eng. 12, 2283 (2006)PubMedCrossRefGoogle Scholar
  42. 42.
    H. Yamasaki, H. Sakai, Biomaterials 13, 308 (1992)PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Sonja Ellen Lobo
    • 1
    • 2
  • Francisco Henrique Lanna Wykrota
    • 3
  • Ana Carolina Marques Barbosa Oliveira
    • 4
  • Irina Kerkis
    • 2
  • Germán Bohorquez Mahecha
    • 1
  • Humberto José Alves
    • 1
  1. 1.Morphology DepartmentFederal University of Minas GeraisBelo HorizonteBrazil
  2. 2.Genetics LaboratoryButantan InstituteSão PauloBrazil
  3. 3.Hospital São Bento Cardioclínica S/A and EINCO Biomaterial Ltd.Minas GeraisBrazil
  4. 4.Federal University of Minas GeraisMinas GeraisBrazil

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