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Multilayer coatings on biomaterials for control of MG-63 osteoblast adhesion and growth

  • Kristin Kirchhof
  • Kamelia Hristova
  • Natalia Krasteva
  • George Altankov
  • Thomas GrothEmail author
Article

Abstract

Here, the layer-by-layer technique (LbL) was used to modify glass as model biomaterial with multilayers of chitosan and heparin to control the interaction with MG-63 osteoblast-like cells. Different pH values during multilayer formation were applied to control their physico-chemical properties. In the absence of adhesive proteins like plasma fibronectin (pFN) both plain layers were rather cytophobic. Hence, the preadsorption of pFN was used to enhance cell adhesion which was strongly dependent on pH. Comparing the adhesion promoting effects of pFN with an engineered repeat of the FN III fragment and collagen I which both lack a heparin binding domain it was found that multilayers could bind pFN specifically because only this protein was capable of promoting cell adhesion. Multilayer surfaces that inhibited MG-63 adhesion did also cause a decreased cell growth in the presence of serum, while an enhanced adhesion of cells was connected to an improved cell growth.

Keywords

Chitosan Heparin Contact Angle Water Contact Angle Biomaterial Surface 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by Graduiertenförderung des Landes Sachsen-Anhalt with a Ph.D. scholarship to KK and the German Academic Exchange Service (DAAD) in the frame of a bilateral exchange program between Germany and Bulgaria. Dr. J. Vogel is acknowledged for scientific support and discussion.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Kristin Kirchhof
    • 1
  • Kamelia Hristova
    • 2
  • Natalia Krasteva
    • 2
  • George Altankov
    • 2
    • 3
  • Thomas Groth
    • 1
    Email author
  1. 1.Biomedical Materials Group, Department of Pharmaceutics and Biopharmaceutics, Institute of PharmacyMartin Luther University Halle-WittenbergHalle (Saale)Germany
  2. 2.Institute of BiophysicsBulgarian Academy of SciencesSofiaBulgaria
  3. 3.ICREA & Institute of Bioengineering CatalunyaBarcelonaSpain

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