Expression of basal lamina components by Schwann cells cultured on poly(lactic acid) (PLLA) and poly(caprolactone) (PCL) membranes

  • A. Pierucci
  • E. A. R. Duek
  • A. L. R. de Oliveira
Article
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Abstract

The present in vitro study investigated the expression of basal lamina components by Schwann cells (SCs) cultivated on PCL and PLLA membranes prepared by solvent evaporation. Cultures of SCs were obtained from sciatic nerves from neonatal Sprague Dawley rats and seeded on 24 well culture plates containing the polymer membranes. The purity of the cultures was evaluated with a Schwann cell marker antibody (anti-S-100). After one week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, laminin I and II. Positive labeling against the studied molecules was observed, indicating that such biomaterials positively stimulate Schwann cell adhesion and proliferation. Overall, the present results provide evidence that membrane-derived biodegradable polymers, particularly those derived from PLLA, are able to provide adequate substrate and stimulate SCs to produce ECM molecules, what may have in turn positive effects in vivo, influencing the peripheral nerve regeneration process.

Keywords

Laminin Schwann Cell PLLA Caprolactone Peripheral Nerve Lesion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

To the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), proc. 471832/2004-9. A.L.R.O. receives a fellowship from CNPq, proc. 304834/2006-8.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • A. Pierucci
    • 1
  • E. A. R. Duek
    • 2
  • A. L. R. de Oliveira
    • 1
  1. 1.Department of Anatomy, Laboratory of Nerve Regeneration, Institute of BiologyUniversity of Campinas––UNICAMPCampinasBrazil
  2. 2.Department of Materials Engineering, Faculty of Mechanical EngineeringUniversity of Campinas––UNICAMPCampinasBrazil

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