Mulberry non-engineered silk gland protein vis-à-vis silk cocoon protein engineered by silkworms as biomaterial matrices

  • Joydip Kundu
  • Moumita Dewan
  • Sarani Ghoshal
  • S. C. Kundu
Article
First Online:
Received:
Accepted:

Abstract

Silk fibroin from silk gland of Bombyx mori 5th instar larvae was utilized to fabricate films, which may find possible applications as two-dimensional matrices for tissue engineering. Bombyx mori cocoon fibroin is well characterized as potential biomaterial by virtue of its good mechanical strength, water stability, thermal properties, surface roughness and biocompatibility. The present study aims to characterize the biophysical, thermal, mechanical, rheological, swelling properties along with spectroscopic analysis, surface morphology and biocompatibility of the silk gland fibroin films compared with cocoon fibroin. Fibroin solutions showed increased turbidity and shear thinning at higher concentration. The films after methanol treatment swelled moderately and were less hydrophilic compared to the untreated. The spectroscopic analysis of the films illustrated the presence of various amide peaks and conformational transition from random coil to β sheet on methanol treatment. X-ray diffraction studies also confirmed the secondary structure. Thermogravimetric analysis showed distinct weight loss of the films. The films were mechanically stronger and AFM studies showed surfaces were rougher on methanol treatment. The matrices were biocompatible and supported L929 mouse fibroblast cell growth and proliferation. The results substantiate the silk gland fibroin films as potential biomaterial matrices.

Keywords

Contact Angle Silk Fibroin Silk Fiber Silk Gland Contact Angle Hysteresis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

SF

Silk fibroin protein

BMG

Bombyx mori gland

BMC

Bombyx mori cocoon

FTIR

Fourier transform infrared

XRD

X-ray diffraction

AFM

Atomic force microscopy

This is a preview of subscription content, log in to check access.

Notes

Acknowledgements

Financial support was obtained from Indo-Australia Biotechnology Fund and Department of Biotechnology, Government of India, New Delhi.

References

  1. 1.
    K. Grzelak, Comp. Biochem. Physiol. B: Biochem. Mol. Biol. 110, 671 (1995)CrossRefGoogle Scholar
  2. 2.
    T. Gamo, Biochem. Genet. 20, 165 (1982)CrossRefGoogle Scholar
  3. 3.
    J.J. Michaille, P. Couble, J.C. Prudhomme, A. Garel, Biochimie 68, 1165 (1986)CrossRefGoogle Scholar
  4. 4.
    S. Inoue, K. Tanaka, F. Arisakaffi, J. Biol. Chem. 275, 40517 (2000)CrossRefGoogle Scholar
  5. 5.
    S. Inoue, J. Magoshi, T. Tanaka, Y. Magoshi, M. Becker, J. Polym. Sci., Part B, Polym. Phys. 38, 1436 (2000)CrossRefGoogle Scholar
  6. 6.
    K. Hu, Q. Lv, F. Cui, Q. Feng, X. Kong, H. Wang, J. Bioact. Compat. Polym. 21, 23 (2006)CrossRefGoogle Scholar
  7. 7.
    T. Arai, G. Freddi, R. Innocenti, M. Tsukada, J. Appl. Polym. Sci. 91, 2383 (2004)CrossRefGoogle Scholar
  8. 8.
    G.H. Altman, F. Diaz, C. Jakuba, T. Calabro, R.L. Horan, J. Chen, Biomaterials 24, 401 (2003)CrossRefGoogle Scholar
  9. 9.
    C. Vepari, D.L. Kaplan, Prog. Polym. Sci. 32, 991 (2007)CrossRefGoogle Scholar
  10. 10.
    S. Hofmann, C.T. Wong Po Foo, F. Rossetti, M. Textor, G. Vunjak-Novakovic, D.L. Kaplan, H.P. Merkle, L. Meinel, J. Control Release 111, 219 (2006)CrossRefGoogle Scholar
  11. 11.
    M. Fini, A. Motta, P. Torricelli, G. Giavaresi, N. Nicoli Aldini, M. Tschon, R. Giardino, C. Migliaresi, Biomaterials 26, 3527 (2005)CrossRefGoogle Scholar
  12. 12.
    T. Hino, M. Tanimoto, S. Shimabayashi, J. Colloid Interface Sci. 266, 68 (2003)CrossRefGoogle Scholar
  13. 13.
    J. Zhu, H. Shao, X. Hu, Int. J. Biol. Macromol. 41, 469 (2007)CrossRefGoogle Scholar
  14. 14.
    A. Sugihara, K. Sugiura, H. Morita, T. Ninagawa, K. Tubouchi, R. Tobe, Proc. Soc. Exp. Biol. Med. 225, 58 (2000)CrossRefGoogle Scholar
  15. 15.
    Y. Wang, D.J. Blasioli, H.J. Kim, H.S. Kim, D.L. Kaplan, Biomaterials 27, 4434 (2006)CrossRefGoogle Scholar
  16. 16.
    S. Rammensee, D. Huemmerich, K.D. Hermanson, T. Scheibel, A.R. Bausch, Appl. Phys. A 82, 261 (2006)CrossRefGoogle Scholar
  17. 17.
    X.X. Feng, L.L. Zhang, J.Y. Chen, Y.H. Guo, H.P. Zhang, C.I. Jia, Int. J. Biol. Macromol. 40, 105 (2007)CrossRefGoogle Scholar
  18. 18.
    S. Putthanarat, S. Zarkoob, J. Magoshi, J.A. Chen, R.K. Eby, M. Stone, W.W. Adams, Polymer 43, 3405 (2002)CrossRefGoogle Scholar
  19. 19.
    N. Agarwal, D.A. Hoagland, R.J. Farris, J. Appl. Polym. Sci. 63, 401 (1997)CrossRefGoogle Scholar
  20. 20.
    Y.Y. Sun, Z.Z. Shao, M.H. Ma, P. Hu, Y.S. Liu, T.Y. Yu, J. Appl. Polym. Sci. 65, 959 (1997)CrossRefGoogle Scholar
  21. 21.
    M.Z. Li, Z.Y. Wu, C.S. Zhang, S.Z. Lu, H.J. Yan, D. Huang, H.L. Ye, J. Appl. Polym. Sci. 79, 2192 (2001)CrossRefGoogle Scholar
  22. 22.
    A.B. Mathur, A. Tonelli, T. Rathke, S. Hudson, Biopolymer 42, 61 (1997)CrossRefGoogle Scholar
  23. 23.
    S. Gobin, V.E. Froude, A.B. Mathur, J. Biomed. Mater. Res. A. 74, 465 (2005)Google Scholar
  24. 24.
    K.E. Park, S.Y. Jung, S.J. Lee, B.M. Min, W.H. Park, Int. J. Biol. Macromol. 38, 165 (2006)CrossRefGoogle Scholar
  25. 25.
    X. Wang, H.J. Kim, P. Xu, A. Matsumoto, D.L. Kaplan, Langmuir 21, 11335 (2005)CrossRefGoogle Scholar
  26. 26.
    R. Nazarov, H.J. Jin, D.L. Kaplan, Biomacromolecules 5, 718 (2004)CrossRefGoogle Scholar
  27. 27.
    R. Valluzzi, S.P. Gido, W. Muller, D.L. Kaplan, Int. J. Biol. Macromol. 24, 237 (1999)CrossRefGoogle Scholar
  28. 28.
    X. Wang, H.J. Kim, P. Xu, A. Matsumoto, D.L. Kaplan, Langmuir 21, 11335 (2005)CrossRefGoogle Scholar
  29. 29.
    J.S. Hwang, J.S. Lee, T.W. Goo, E.Y. Yun, K.S. Lee, Y.S. Kim, B.R. Jin, S.M. Lee, K.Y. Kim, S.W. Kang, D.S. Suh, Biotech. Lett. 231, 1321 (2001)CrossRefGoogle Scholar
  30. 30.
    W. Wang, S. Zhu, L. Wang, F. Yu, W. Shen, J. Biosci. 30, 605 (2005)CrossRefGoogle Scholar
  31. 31.
    B.B. Mandal, S.C. Kundu, Biotechnol.Bioeng. in pressGoogle Scholar
  32. 32.
    H. Shiozaki, Y. Tanaka, Angewandte Makromolekulare Chemie 64, 1 (2003)CrossRefGoogle Scholar
  33. 33.
    M.L. Gimenes, L. Liu, X. Feng, J. Memb. Sci. 29, 71 (2007)CrossRefGoogle Scholar
  34. 34.
    X. Wang, X. Hu, A. Daley, O. Rabotyagova, P. Cebe, D.L. Kaplan, J. Control Release 121, 190 (2007)CrossRefGoogle Scholar
  35. 35.
    O. Bayraktar, Ö. Malay, Y. Özgarip, A. batigün, Eur. J. Pharm. Biopharm. 60, 373 (2005)CrossRefGoogle Scholar
  36. 36.
    H.J. Jin, J. Park, V. Karageorgiou, U.J. Kim, R. Valluzzi, P. Cebe, D.L. Kaplan, Adv. Func. Mater. 15, 1241 (2005)CrossRefGoogle Scholar
  37. 37.
    ASTM, Annual Book of ASTM Standards. (Philadelphia, PA, USA, 1993), p. 59Google Scholar
  38. 38.
    O. Malay, O. Bayraktar, A. Batiguin, Int. J. Biol. Macromol. 40, 387 (2007)CrossRefGoogle Scholar
  39. 39.
    D.L. Kaplan, S. Fossey, C.M. Mello, Mat. Res. Soc. Bull. 17, 41 (1992)Google Scholar
  40. 40.
    C.Z. Zhou, F. Confalonieri, N. Medina, Y. Zivanovic, C. Esnault, T. Yang, M. Jacquet, J. Janin, M. Duguet, R. Perasso, Z.G. Li, Nucleic Acids Res. 28, 2413 (2000)CrossRefGoogle Scholar
  41. 41.
    J.H. Yeo, K.G. Lee, Y.W. Lee, S.Y. Kim, Eur. Polym. J. 39, 1195 (2003)CrossRefGoogle Scholar
  42. 42.
    M.K. Yoo, H.Y. Kweon, K.G. Lee, H.C. Lee, C.S. Cho, Int. J. Biol. Macromol. 34, 263 (2004)CrossRefGoogle Scholar
  43. 43.
    I.C. Um, H.Y. Kweon, Y.H. Park, S. Hudson, Int. J. Biol. Macromol. 29, 91 (2001)CrossRefGoogle Scholar
  44. 44.
    H. Wang, Y. Zhang, H. Shao, X. Hu, Int. J. Biol. Macromol. 36, 66 (2005)CrossRefGoogle Scholar
  45. 45.
    D.L. Kaplan, S.M. Mello, S. Arcidiacono, S. Fossey, K.W.M. Senecal, Protein Based Materials. (Birkhauser, Boston, 1998), p. 103Google Scholar
  46. 46.
    H.J. Jin, D.L. Kaplan, Nature 424, 1057 (2003)CrossRefGoogle Scholar
  47. 47.
    A. Motta, L. Fambri, C. Migliaresi, Macromol. Chem. Phys. 203, 1658 (2002)CrossRefGoogle Scholar
  48. 48.
    N. Miniura, S. Aiba, M. Higuchi, Biochem. Biophys. Res. Comm. 208, 511 (1995)CrossRefGoogle Scholar
  49. 49.
    M. Tsukada, Y. Gotoh, M. Nagura, N. Minoura, N. Kasai, G. Freddi, J. Polym. Sci. B: Polym. Phys. 32, 961 (1994)CrossRefGoogle Scholar
  50. 50.
    J. Zhu, H. Shao, X. Hu, Int. J. Biol. Macromol. 41, 469 (2007)CrossRefGoogle Scholar
  51. 51.
    W. Tao, M. Li, C. Zhao, Int. J. Biol. Macromol. 40, 472 (2007)CrossRefGoogle Scholar
  52. 52.
    T. Iliescu, D. Maniu, V. Chis, F.D. Irimie, C.S. Paizs, M. Tosa, Chem. Phys. 310, 189 (2005)CrossRefGoogle Scholar
  53. 53.
    M.E. Rousseau, T. Lefèvre, L. Beaulieu, T. Asakura, M. Pézolet, Biomacromolecules 56, 2247–2257 (2004)CrossRefGoogle Scholar
  54. 54.
    J.W. Van Egmond, Curr. Opin. Colloid Interface Sci. 3, 385 (1998)CrossRefGoogle Scholar
  55. 55.
    X. Chen, D.P. Knight, Z. Shao, F. Vollrath, Polymer 42, 9969 (2001)CrossRefGoogle Scholar
  56. 56.
    K. Cai, K. Yao, Y. Cui, Z. Yang, X. Li, H. Xie, T. Qing, L. Gao, Biomaterials 23, 1603 (2002)CrossRefGoogle Scholar
  57. 57.
    A.M. Tellez-Garay, M.Sc. Thesis, Texas A&M University, College Station, TX (1999)Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Joydip Kundu
    • 1
  • Moumita Dewan
    • 1
  • Sarani Ghoshal
    • 1
  • S. C. Kundu
    • 1
  1. 1.Department of BiotechnologyIndian Institute of TechnologyKharagpurIndia

Personalised recommendations