Preparation and blood coagulation evaluation of chitosan microspheres

  • Qi Zhao Wang
  • Xi Guang Chen
  • Zi Xiang Li
  • Song Wang
  • Cheng Sheng Liu
  • Xiang Hong Meng
  • Chen Guang Liu
  • Ying Hui Lv
  • Le Jun Yu
Article

DOI: 10.1007/s10856-007-3243-y

Cite this article as:
Wang, Q.Z., Chen, X.G., Li, Z.X. et al. J Mater Sci: Mater Med (2008) 19: 1371. doi:10.1007/s10856-007-3243-y

Abstract

Cross-linked chitosan microspheres (40–100 μm) with smooth surface were prepared by the methods of emulsification and ethanol coagulant. FTIR results showed that the cross-linking reaction occurred on the amino groups of chitosan molecules. The swelling characteristic of chitosan microspheres was influenced by the environment pH, being generally greater at low rather than higher pH values. The coagulation properties of chitosan microspheres were evaluated by dynamic blood clotting, platelet adhesion and activation, erythrocyte adhesion, hemolysis, and protein absorption assays. Chitosan microspheres can shorten the clotting time and induce the adhesion and activation of platelets. But the shortening of clotting time by chitosan microspheres may be related to not only platelet aggregation, but also erythrocyte aggregation. Take together, chitosan microspheres may be potential use as thrombospheres.

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Qi Zhao Wang
    • 1
    • 2
  • Xi Guang Chen
    • 1
  • Zi Xiang Li
    • 3
  • Song Wang
    • 3
  • Cheng Sheng Liu
    • 1
  • Xiang Hong Meng
    • 1
  • Chen Guang Liu
    • 1
  • Ying Hui Lv
    • 1
    • 2
  • Le Jun Yu
    • 1
  1. 1.College of Marine Life ScienceOcean University of ChinaQingdaoP.R. China
  2. 2.Institute of Molecular MedicineHuaqiao UniversityQuanzhouP.R. China
  3. 3.The Affiliated Hospital of Medical CollegeQingdao UniversityQingdaoP.R. China

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