Genipin-crosslinked chitosan/gelatin blends for biomedical applications

  • Valeria Chiono
  • Ettore Pulieri
  • Giovanni Vozzi
  • Gianluca Ciardelli
  • Arti Ahluwalia
  • Paolo Giusti
Article

Abstract

Blends between chitosan (CS) and gelatin (G) with various compositions (CS/G 0/100 20/80, 40/60, 60/40 100/0 w/w) were produced, as candidate materials for biomedical applications. Different amounts of genipin (0.5 wt.% and 2.5 wt.%) were used to crosslink CS/G blends, promoting the formation of amide and tertiary amine bonds between the macromolecules and the crosslinker. The effects of composition and crosslinking on the physico-chemical properties of samples were evaluated by infrared analysis, thermogravimetry, contact angle measurements, dissolution and swelling tests. Mechanical properties of crosslinked samples were also determined through stress–strain and creep tests: samples stiffness increased with increasing the crosslinker amount and the CS content. Blend composition affected mouse fibroblasts adhesion and proliferation on substrates, depending on the crosslinker amount. Finally, crosslinked CS/G blends containing 80 wt.% G were found to support neuroblastoma cells adhesion and proliferation which made them promising candidates for uses in the field of nerve regeneration.

References

  1. 1.
    W. W. MINUTH, M. SITTINGER and S. KLOTH, Cell. Tissue Res. 291 (1998) 1CrossRefGoogle Scholar
  2. 2.
    D. M. BARNES, Science 230 (1985) 1024CrossRefGoogle Scholar
  3. 3.
    Y. YUAN, P. ZHANG, Y. YANG, X. WANG and X. GU, Biomaterials 25 (2004) 4273CrossRefGoogle Scholar
  4. 4.
    R. P. BUNGE, J. Neural. 241 (1994) S 19Google Scholar
  5. 5.
    S. ITOH, I. YAMAGUCHI, M. SUZUKI, S. ICHINOSE, K. TAKAKUDA, H. KOBAYASHI, K. SHINOMIYA and J. TANAKA, Brain Res. 993 (2003) 111CrossRefGoogle Scholar
  6. 6.
    M. CHENG, J. DENG, F. YANG, Y. GONG, N. ZHAO and X. ZHANG, Biomaterials 24 (2003) 2871CrossRefGoogle Scholar
  7. 7.
    M. CHENG, W. CAO, Y. GAO, Y. GONG, N. ZHAO and X. ZHANG, J. Biomater. Sci. Polym. Ed. 14 (2003) 1155CrossRefGoogle Scholar
  8. 8.
    F. -H. LIN, C. -H. YAO, J. -S. SUN, H. -C. LIU and C. -W. HUANG, Biomaterials 19 (1998) 905CrossRefGoogle Scholar
  9. 9.
    P. -R. CHEN, M. -H. CHEN, J. -S. SUN, M. -H. CHEN, C. -C. TSAI and F. -H. -F. -H. LIN, Biomaterials 25 (2004) 5667CrossRefGoogle Scholar
  10. 10.
    J. MAO, L. ZHAO, K. D. YAO, Q. SHANG, G. YANG and Y. CAO, J. Biomed. Mater. Res. 64A (2003) 301CrossRefGoogle Scholar
  11. 11.
    Y. HANG, S. ONYERI, M. SIEWE, A. MOSHFEGHIAN and S. V. MADIHALLY, Biomaterials 26 (2005) 7616CrossRefGoogle Scholar
  12. 12.
    E. CHIELLINI, P. CINELLI, E. GRILLO FERNANDES, el-R. -S. KENAWAY and A. LAZZERI, Biomacromolecules 3 (2001) 806CrossRefGoogle Scholar
  13. 13.
    M. A. VANDELLI, M. ROMAGNOLI, A. MONTI, M. GOZZI, P. GUERRA, F. RIVASI and F. FORNI, J. Control. Release 96 (2004) 67CrossRefGoogle Scholar
  14. 14.
    V. YANNAS and A. V. TOBOLSKY, Nature 215 (1967) 509CrossRefGoogle Scholar
  15. 15.
    H. UEDA, T. NAKAMURA, M. YAMAMOTO, N. NAGATA, S. FUKUDA, Y. TABATA and Y. SHIMIZU, J. Control. Release. 88 (2000) 55CrossRefGoogle Scholar
  16. 16.
    M. M. WELZ and C. M. OFNER III, J. Pharm. Sci. 81 (1992) 85CrossRefGoogle Scholar
  17. 17.
    L. MA, C. GAO, Z. MAO, J. SHEN, X. HU and C. HAN, J. Biomater. Sci. Polym. Ed. 14 (2003) 861CrossRefGoogle Scholar
  18. 18.
    G. A. DIOGENIS, T. B. GOLD and V. P. SHAH, J. Pharm. Sci. 83 (1994) 915CrossRefGoogle Scholar
  19. 19.
    T. MIYADA, T. TARA and Y. NOISHIKI, Clin. Mater. 9 (1992) 139CrossRefGoogle Scholar
  20. 20.
    A. J. KUIJPERS, G. H. M. ENGBERS, J. KRIJGSVELD, S. A. J. ZAAT, J. DANKERT and J. FEIJEN, J. Biomater. Sci. Polym. Ed. 11 (2000) 225CrossRefGoogle Scholar
  21. 21.
    A. BIGI, G. COJAZZI, S. PANZAVOLTA, N. ROVERI and K. RUBINI, Biomaterials 23 (2002) 4827CrossRefGoogle Scholar
  22. 22.
    T. AKAO, K. KOBASHI and M. ABURABA, Biol. Pharm. Bull. 17 (1994) 1573Google Scholar
  23. 23.
    Y. S. PAIK, C. M. LEE, M. H. CHO and T. R. HAHN, J. Agric. Food Chem. 49 (2001) 430CrossRefGoogle Scholar
  24. 24.
    J. JIN, M. SONG and D. J. HOURSTON, Biomacromolecules 5 (2004) 162CrossRefGoogle Scholar
  25. 25.
    F. -L. MI, Y. -C. TAN, H. -F. LIANG and H. -W. SUNG, Biomaterials 23 (2002) 181CrossRefGoogle Scholar
  26. 26.
    F. -L. MI, H. -W. SUNG, S. -S. SHYU, C. -C. SU and C. -K. PENG, Polymer 44 (2003) 6521CrossRefGoogle Scholar
  27. 27.
    Y. -S. CHEN, J. -Y. CHANG, C. -Y. CHENG, F. -J. TSAI, C. -H. YAO and B. -S. LIU, Biomaterials 26 (2005) 3065CrossRefGoogle Scholar
  28. 28.
    H. W. SUNG, R. N. HUANG, L. L. H. HUANG and C. C. TSAI, J. Biomater. Sci. Polym. Ed. 10 (1999) 63Google Scholar
  29. 29.
    C. C. TSAI, R. N. HUANG, H. W. SUNG and H. C. LIANG, J. Biomed. Mater. Res. 52 (2000) 58CrossRefGoogle Scholar
  30. 30.
    H. W. SUNG, D. M. HUANG, W. H. CHANG, L. L. HUANG, C. C. TSAI and I. L. LIANG, J. Biomater. Sci. Polym. Ed. 10 (1999) 751Google Scholar
  31. 31.
    H. W. SUNG, D. M. HUANG, W. H. CHANG, R. N. HUANG and J. C. HSU, J. Biomed. Mater. Res. 46 (1999) 520CrossRefGoogle Scholar
  32. 32.
    H. W. SUNG, R. N. HUANG, L. L. H. HUANG, C. C. TSAI and C. T. CHIU, J. Biomed. Mater. Res. 42 (1998) 560CrossRefGoogle Scholar
  33. 33.
    M. F. BUTLER, Y. -F. NG and P. D. A. PUDNEY, J. Polym. Sci. Part A: Polym. Chem. 41 (2003) 3941CrossRefGoogle Scholar
  34. 34.
    C. ABRUSCI, A. MARTIN-GONZALEZ, A. DEL AMO, T. CORRALES and F. CATALINA, Polym. Degrad. Stability. 86 (2004) 283CrossRefGoogle Scholar
  35. 35.
    G. CIARDELLI, V. CHIONO, G. VOZZI, M. PRACELLA, A. AHLUWALIA, N. BARBANI, C. CRISTALLINI and P. GIUSTI, Biomacromolecules 6 (2005) 1961CrossRefGoogle Scholar
  36. 36.
    G. VOZZI, C. J. FLAIM, F. BIANCHI, A. AHLUWALIA and S. N. BHATIA, Mater Sci Eng. C20 (2002) 43Google Scholar
  37. 37.
    E. PULIERI, V. CHIONO, G. CIARDELLI, G. VOZZI, A. AHLUWALIA, C. DOMENICI, F. VOZZI and P. GIUSTI, J. Biomed. Mater. Res. Part A. (in press)Google Scholar
  38. 38.
    C. PENICHE, C. ELVIRA and J. S. ROMAN, Polymer 39 (1998) 6549CrossRefGoogle Scholar
  39. 39.
    A. SIONKOWSKA, M. WISNIEWSKI, J. SKOPINSKA, C. J. KENNEDY and T. J. WESS, Biomaterials 25 (2004) 795CrossRefGoogle Scholar
  40. 40.
    P. B. van WACHEM, T. BEUGELING, J. FEJIEN, A. BANTJES, J. P. DETMERS and W. G. van AKEN, Biomaterials 6 (1985) 403CrossRefGoogle Scholar
  41. 41.
    J. M. BELLON, L. A. CONTRERAS, C. SABATER and J. BUJAN, World J. Surg. 21 (1997) 402CrossRefGoogle Scholar
  42. 42.
    R. HAIGH, N. FULLWOOD and S. RIMMER, Biomaterials 23 (2002) 3509CrossRefGoogle Scholar
  43. 43.
    G. CIARDELLI and V. CHIONO, Macromol. Biosci. 6 (2006) 13CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Valeria Chiono
    • 1
  • Ettore Pulieri
    • 2
  • Giovanni Vozzi
    • 1
    • 2
  • Gianluca Ciardelli
    • 1
    • 3
  • Arti Ahluwalia
    • 2
  • Paolo Giusti
    • 1
    • 4
  1. 1.Department of Chemical Engineering, Industrial Chemistry and Materials ScienceUniversity of PisaPisaItaly
  2. 2.Centro “E. Piaggio”University of PisaPisaItaly
  3. 3.Department of MechanicsPolitecnico in TurinTurinItaly
  4. 4.C.N.R. Institute for Composite and Biomedical MaterialsPisaItaly

Personalised recommendations