Journal of Materials Science: Materials in Medicine

, Volume 19, Issue 2, pp 877–882

STEM-HAADF electron microscopy analysis of the central dark line defect of human tooth enamel crystallites

  • Jose Reyes Gasga
  • Georgina Carbajal-de-la-Torre
  • Etienne Bres
  • Ivet M. Gil-Chavarria
  • Ana G. Rodríguez-Hernández
  • Ramiro Garcia-Garcia
Article

Abstract

When human tooth enamel is observed with the Transmission Electron Microscope (TEM), a structural defect is registered in the central region of their nanometric grains or crystallites. This defect has been named as Central Dark Line (CDL) and its structure and function in the enamel structure have been unknown yet. In this work we present the TEM analysis to these crystallites using the High Angle Annular Dark Field (HAADF) technique. Our results suggest that the CDL region is the calcium richest part of the human tooth enamel crystallites.

References

  1. 1.
    E. RÖNNHOLM, J. Ultrastruct. Res. 6 (1962) 249CrossRefGoogle Scholar
  2. 2.
    M. U. NYLEN, E. D. EANES and K. A. OMNELL, J. Cell. Biol. 18 (1963) 109CrossRefGoogle Scholar
  3. 3.
    A. F. MARSHALL and K. R. LAWLESS, J. Dent. Res. 60 (1981) 1773Google Scholar
  4. 4.
    D. G. A. NELSON, G. J. WOOD, J. C. BARRY and J. D. B. FEATHERSTONE, Ultramicroscopy 19 (1986) 253CrossRefGoogle Scholar
  5. 5.
    E. F. BRES, W. G. WADDINGTON, J. C. VOEGEL, J. C. BARRY and R. M. FRANK, Biophysics 1 (1986) 1105Google Scholar
  6. 6.
    T. YANAGISAWA and Y. MIAKE, J. Electron Microsc. 52 (2003) 605CrossRefGoogle Scholar
  7. 7.
    E. F. BRÈS, J. L. HUTCHISON, J.-C. VOEGEL and R. M. FRANK, Journal de Physique 51 (1990) C1-97Google Scholar
  8. 8.
    E. F. BRÈS, W. G. WADDINGTON, J.-C. VOEGEL, J. C. BARRY and R. M. FRANK, Biophys. J. 50 (1986) 1185CrossRefGoogle Scholar
  9. 9.
    E. F. BRÈS, J. C. BARRY and J. L. HUTCHISON, Ultramicroscopy 12 (1984) 367CrossRefGoogle Scholar
  10. 10.
    P. BODIER-HOULLÉ, P. STEUER, J.-C. VOEGEL and F. J. G. CUISINIER, Acta Cryst. D54 (1998) 1377Google Scholar
  11. 11.
    C. ROBINSON, S. CONNELL, J. KIRKHAM, R. SHORE and A. SMITH, J. Mater. Chem. 14 (2004) 2242CrossRefGoogle Scholar
  12. 12.
    D. B. SCOTT, J. W. SIMMELINK and V. NYGAARD, J. Dent. Res. 53 (1974) 165Google Scholar
  13. 13.
    J.-C. VOEGEL and R. M. FRANK, Calcif. Tissue Res. 24 (1997) 19CrossRefGoogle Scholar
  14. 14.
    A. BELIO-REYES, L. VARGAS-ULLOA, L. F. JIMENEZ-GARCIA and J. REYES-GASGA, Latin-Am. J. Metallurgy Mater. 19 (1999) 52Google Scholar
  15. 15.
    M. S. ISAACSON, M. OHTSUKI and M. UTLAUT, in “Introduction to the Analytical Electron Microscopy”, edited by J. J. HREN, J. I. GOLDSTEIN and D. C. JOY (Plenum Press, New York, 1979) p. 343Google Scholar
  16. 16.
    V. CREWE, J. P. LANGMORE and M. S. ISAACSON, in “Physical Aspects of Electron Microscopy and Microbeam Analysis”, edited by B. M. SIEGEL and D. R. BEMAN (Wiley, New York, 1975) p. 47Google Scholar
  17. 17.
    A. Howie, J. Microsc. 117 (1979) 11Google Scholar
  18. 18.
    J. M. COWLEY, Ultramicroscopy 2 (1976) 258CrossRefGoogle Scholar
  19. 19.
    S. J. PENNYCOOK, S. D. BERGER and R. J. CULBERTSON, J. Microsc. 144 (1986) 229Google Scholar
  20. 20.
    S. J. PENNYCOOK and D. E. JESSON, Ultramicroscopy 37 (1991) 14CrossRefGoogle Scholar
  21. 21.
    J. REYES-GASGA and R. GARCIA-GARCIA, Radiat. Phys. Chem. 64 (2002) 359CrossRefGoogle Scholar
  22. 22.
    L. F. LOZANO, M. A. PEÑA-RICO, A. HEREDIA, J. OCOTLAN-FLORES, A. GOMEZ-CORTES, R. VELÁSQUEZ, I. A. BELIO and L. BUCIO, J. Mater. Sci. 38 (2003) 4777CrossRefGoogle Scholar
  23. 23.
    C. P. LIN, B. S. LEE, S. H. KOK, W. H. LAN, Y. C. TSENG and F. H. LIN, J. Mat. Sci. 11 (2000) 373Google Scholar
  24. 24.
    F. H. LIN, C. J. LIAO, K. S. CHEN and J. S. SUN, Biomaterials 19 (1998) 1101CrossRefGoogle Scholar
  25. 25.
    M. IIJIMA, H. THODA and Y. MORIWAKI, J. Cryst. Growth 116 (1992) 319CrossRefGoogle Scholar
  26. 26.
    M. IIJIMA and Y. MORIWAKI, J. Cryst. Growth 194 (1998) 125; J. Cryst. Growth 198199 (1999) 670Google Scholar
  27. 27.
    Y. MIAKE, S. SHIMODA, M. FUKAE and T. AOBA, Calcif. Tissue Int. 53 (1993) 249CrossRefGoogle Scholar
  28. 28.
    A. G. RODRIGUEZ-HERNANDEZ, M. E. FERNANDEZ, G. CARBAJAL DE LA TORRE, R. GARCIA-GARCIA and J. REYES-GASGA, in “Electron Microscopy of Molecular and Atom-Scale Mechanical Behavior, Chemistry and Structure”, edited by D. C. MARTIN, D. A. MULLER, P. A. MIDGLEY and E. A. STACH, Mater. Res. Soc. Symp. Proc. 839 (2005), P6.10.1Google Scholar
  29. 29.
    M. E. FERNÁNDEZ, J. A. ASCENCIO, C. ZORRILLA-CANGAS, R. GARCÍA-GARCÍA and J. REYES-GASGA, Acta Cryst. B 59 (2003) 175CrossRefGoogle Scholar
  30. 30.
    G. H. NANCOLLAS, Pure Appl. Chem. 64 (1992) 1673CrossRefGoogle Scholar
  31. 31.
    P. P. PHAKEY and J. R. LEONARD, J. Appl. Cryst. 3 (1970) 38CrossRefGoogle Scholar
  32. 32.
    M. RÜHLE and S. L. SASS, Philos. Mag. A 49 (1984) 759CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Jose Reyes Gasga
    • 1
  • Georgina Carbajal-de-la-Torre
    • 1
    • 2
  • Etienne Bres
    • 2
  • Ivet M. Gil-Chavarria
    • 1
    • 3
  • Ana G. Rodríguez-Hernández
    • 1
    • 3
  • Ramiro Garcia-Garcia
    • 1
  1. 1.Instituto de FísicaUNAMMexicoMexico
  2. 2.Laboratorie de Structure et des Proprietes del 1´Etat Solide CNRS UMR 8008Universite des Scinces et des Technologies de LilleVillenueve d’AscqFrance
  3. 3.Facultad de OdontologíaUNAM, Unidad de Posgrado, Circuito de la Investigación Científica, Cd, UniversitariaCoyoacan, MexicoMexico

Personalised recommendations