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Gelatin sponges (Gelfoam®) as a scaffold for osteoblasts

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Abstract

Gelatine sponge because of its flexibility, biocompatibility, and biodegradability, has the potential to be used as a scaffold to support osteoblasts and to promote bone regeneration in defective areas. This study aimed to determine osteoblast proliferation, differentiation, and integration in modified and un-modified gelatine sponges. Three scaffolds were studied: gelatine sponge (Gelfoam®), gelatin sponge/mineral (hydroxyapatite) composite, and gelatin sponge/polymer (poly-lactide-co-glycolide) composite. 2–D plastic coverslip was used as control. The gelatin sponges were modified using PLGA coating and mineral deposition to increase biodegradation resistance and osteoblast proliferation respectively. The scaffolds were characterized using Scanning Electron Microscopy (SEM) and X-ray diffraction. Cell number (DNA content), cell-replication rate (thymidine assay), and cell differentiation (alkaline phosphatase activity) were measured 24 h, 3 days, and 1, 2, 3 weeks after the osteoblast-like cells were cultured onto the scaffolds. Cell penetration into the sponges was determined using haematoxylin-eosin staining. Both modified and unmodified gelatine sponges demonstrated ability to support cell growth and cells were able to penetrate into the sponge pores. In a comparison of different scaffolds, cell number and cell replication were highest in sponge/hydroxyapatite composite and lowest in sponge/PLGA composite.

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Acknowledgment

This project was supported by a project grant from National Health and Medical Research Council (NHMRC), Australia. We wish to thank the Electron Microscopy Unit (EMU) of the University of Sydney, Australia, for their expert technical assistance.

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Authors and Affiliations

  1. Bone and Skin Research Group, Department of Physiology, University of Sydney, Sydney, NSW, Australia

    Ramin Rohanizadeh & Rebecca S. Mason

  2. Advanced Drug Delivery Group, Faculty of Pharmacy, University of Sydney, Sydney, NSW, Australia

    Ramin Rohanizadeh

  3. Biomaterials Unit, Faculty of Dentistry, University of Sydney, Sydney, NSW, Australia

    Michael V. Swain

Authors
  1. Ramin Rohanizadeh
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  2. Michael V. Swain
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  3. Rebecca S. Mason
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Correspondence to Ramin Rohanizadeh.

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Rohanizadeh, R., Swain, M.V. & Mason, R.S. Gelatin sponges (Gelfoam®) as a scaffold for osteoblasts. J Mater Sci: Mater Med 19, 1173–1182 (2008). https://doi.org/10.1007/s10856-007-3154-y

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  • DOI: https://doi.org/10.1007/s10856-007-3154-y

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