Effect of biological implant surface coatings on bone formation, applying collagen, proteoglycans, glycosaminoglycans and growth factors

  • Bernd Stadlinger
  • Eckart Pilling
  • Ronald Mai
  • Susanne Bierbaum
  • Ricardo Berhardt
  • Dieter Scharnweber
  • Uwe Eckelt



The aim of the present study was to evaluate six different implant surface coatings with respect to bone formation. Being major structural components of the extracellular matrix, collagen, the non-collagenous components decorin/chondroitin sulphate (CS) and the growth factors TGF-β1/BMP-4 served in different combinations as coatings of experimental titanium implants.

Materials and methods

Eight miniature pigs received each six implants in the mandible. The implant design showed two circular recesses along the length axis. Three, four, five and six weeks after implant placement, the animals were sacrificed in groups of two. Bone-implant contact (BIC) was evaluated along the outer implant surface and within the recesses. Bone volume was determined by synchrotron radiation micro computed tomography (SRμCT) for one implant of each surface state, 6 weeks after placement.


At each week of observation, collagen/CS or collagen/CS/BMP-4 coated implants showed the highest BIC of all surface states. This was statistically significant at week five (p = 0.030, p = 0.040) and six (p = 0.025, p = 0.005). SRμCT measurements determined the highest bone volume for a collagen/CS coated implant.


The results indicate that collagen/CS and collagen/CS/BMP-4 lead to a higher degree of bone formation compared to other ECM components.


Bone Morphogenetic Protein Bone Volume Titanium Implant Coated Implant Recombinant Growth Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The financial support of the “Deutsche Forschungsgemeinschaft” is gratefully acknowledged. The authors further thank Dr. Roland Jung, Centre for Experimental Studies Dresden, Mrs. Ines Kleiber, Histological Laboratory and Dipl.math. Eberhardt Kuhlisch, Insitute for Medical Informatics and Biometry, for the extensive assistance in the animal study.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Bernd Stadlinger
    • 1
  • Eckart Pilling
    • 1
  • Ronald Mai
    • 1
  • Susanne Bierbaum
    • 2
  • Ricardo Berhardt
    • 2
  • Dieter Scharnweber
    • 2
  • Uwe Eckelt
    • 1
  1. 1.Department of Oral & Maxillofacial Surgery, Faculty of MedicineUniversity of Technology DresdenDresdenGermany
  2. 2.Max Bergmann Centre of Biomaterials, Institute of Materials ScienceUniversity of Technology DresdenDresdenGermany

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