Stereological analysis of the poly-(dl-lactide-co-glycolide) submicron sphere prepared by solvent/non-solvent chemical methods and centrifugal processing
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Fine particles made of poly(lactide-co-glycolide) (DLPLG) are excellent candidates for controlled release of delivering drugs and genes, because of their degradable nature. The preparation of DLPLG submicron spheres poses serious challenges that are not necessarily present when preparing macroparticles. In the present paper, DLPLG powder is produced with chemical method using solvent/non-solvent systems with subsequent centrifugation of the solution. The samples were characterized by infrared spectroscopy (IR), scanning electron microscopy (SEM) and stereological analysis. By changing the aging time with non-solvent and time and velocity of the centrifugal processing, it is possible to influence on the morphology and uniformity of the copolymer particles. Powder of the series with short aging time with non-solvent and longest time and velocity of the centrifugal processing has smallest particles and highest uniformity, where mean particles sizes were between 150 nm and 230 nm depending on which stereological parameters are considered (D max, maximal diameters, feret X or feret Y).
KeywordsSupercritical Fluid Extraction Stereological Analysis Emulsion Process Copolymer Poly Centrifugal Processing
Authors would like to thank Zoran Nedic and Milos Bokorov for their help in IR and SEM analysis. The Ministry of Science and Environmental Protection of the Republic of Serbia supports this work through the project No. 142006.
- 1.B. D. RATNER, A. S. HOFFMAN, F. J. SCHOEN AND J. E. LEMONS (Eds.), Biomaterials Science, Elsevier Inc, (2004) p. 864Google Scholar
- 8.A. G. A. COOMBES, S. S. DAVIS and E. H. SCHACHT, United States Patent, 5.922.357, (1999)Google Scholar
- 16.U. B. KOMPELLA and K. KOUSHIK, Crit. Rev. Ther. Drug. Carrier. Syst. 18 (2001) 173Google Scholar
- 23.J. OLIVIER, J. Am. Soc. Exp. Neurother. 2 (2005) 108Google Scholar
- 24.E. E. UNDERWOOD, Quantitative stereology. Addison Wesley, Reading, M, (1969) p. 330Google Scholar
- 26.M. KIREMITCI-GUMUSDERELIOGLU and G. DENIZ, Turk. J. Chem. 23 (1999) 153Google Scholar