Premixed macroporous calcium phosphate cement scaffold

  • Hockin H. K. Xu
  • Lisa E. Carey
  • Carl G. SimonJr.
Article

Abstract

Calcium phosphate cement (CPC) sets in situ to form resorbable hydroxyapatite and is promising for orthopaedic applications. However, it requires on-site powder-liquid mixing during surgery, which prolongs surgical time and raises concerns of inhomogeneous mixing. The objective of this study was to develop a premixed CPC scaffold with macropores suitable for tissue ingrowth. To avoid the on-site powder-liquid mixing, the CPC paste was mixed in advance and did not set in storage; it set only after placement in a physiological solution. Using 30% and 40% mass fractions of mannitol porogen, the premixed CPC scaffold with fibers had flexural strength (mean ± sd; n = 5) of (3.9 ± 1.4) MPa and (1.8 ± 0.8) MPa, respectively. The scaffold porosity reached (68.6 ± 0.7)% and (74.7 ± 1.2)%, respectively. Osteoblast cells colonized in the surface macropores of the scaffold and attached to the hydroxyapatite crystals. Cell viability values for the premixed CPC scaffold was not significantly different from that of a conventional non-premixed CPC known to be biocompatible (P > 0.1). In conclusion, using fast-dissolving porogen and slow-dissolving fibers, a premixed macroporous CPC scaffold was developed with strength approaching the reported strengths of sintered porous hydroxyapatite implants and cancellous bone, and non-cytotoxicity similar to a biocompatible non-premixed CPC.

Notes

Acknowledgments

We gratefully thank Dr. S. Takagi for discussions and experimental help, and Drs. L. C. Chow, F. C. Eichmiller, and S. H. Dickens for discussions. This study was supported by USPHS grant DE14190 (Xu), Y1-DE-1021 (Simon), NIST, and the ADAF.

Disclaimer

Certain commercial materials and equipment are identified to specify experimental procedures. In no instance does such identification imply recommendation by NIST or the ADA Foundation or that the material identified is necessarily the best available for the purpose.

References

  1. 1.
    L. L. HENCH, J. WILSON, in “Introduction to Bioceramics” (World Scientific, Singapore, 1993)Google Scholar
  2. 2.
    R. Z. LEGEROS, Clinical. Mater. 14 (1993) 65CrossRefGoogle Scholar
  3. 3.
    K. A. HING, S. M. BEST and W. BONFIELD, J. Mater. Sci.: Mater. in Med. 10 (1999) 135CrossRefGoogle Scholar
  4. 4.
    W. SUCHANEK and M. YOSHIMURA, J. Mater. Res. 13 (1998) 94CrossRefGoogle Scholar
  5. 5.
    C. T. LAURENCIN, A. M. AMBROSIO, M. D. BORDEN and J. A. COOPER, Annu. Rev. Biomed. Eng. 1 (1999) 19CrossRefGoogle Scholar
  6. 6.
    W. E. Brown, L. C. Chow, In Cements research progress, edited by P. W. Brown (American Ceramic Society, Westerville, OH, 1986) p. 352Google Scholar
  7. 7.
    M. P. GINEBRA, E. FERNANDEZ, E. A. De MAEYER, R. M. VERBEECK, M. G. BOLTONG, J. GINEBRA, F. C. DRIESSENS and J. A. PLANELL, J. Dent. Res. 76 (1997) 905Google Scholar
  8. 8.
    Y. MIYAMOTO, K. ISHIKAWA, M. TAKECHI, T. TOH, T. YUASA, M. NAGAYAMA and K. SUZUKI, J. Biomed. Mater. Res. (Appl. Biomater.) 48 (1999) 36CrossRefGoogle Scholar
  9. 9.
    L. C. CHOW, Mat. Res. Symp. Proc. 599 (2000) 27Google Scholar
  10. 10.
    P. D. COSTANTINO, C. D. FRIEDMAN, K. JONES, L. C. CHOW and G. A. SISSON, Plast. Reconstr. Surg. 90 (1992) 174Google Scholar
  11. 11.
    C. D. FRIEDMAN, P. D. COSTANTINO, S. TAKAGI and L. C. CHOW, J. Biomed. Mater. Res. (Appl. Biomater.) 43 (1998) 428CrossRefGoogle Scholar
  12. 12.
    H. H. K. XU, J. B. QUINN, S. TAKAGI and L. C. CHOW, J. Dent. Res. 81 (2002) 219CrossRefGoogle Scholar
  13. 13.
    H. H. K. XU, S. TAKAGI, J. B. QUINN and L. C. CHOW, J. Biomed. Mater. Res. 68A (2004) 725CrossRefGoogle Scholar
  14. 14.
    H. H. K. XU and C. G. SIMON Jr, J. Orthop. Res. 22 (2004) 535CrossRefGoogle Scholar
  15. 15.
    S. TAKAGI, L. C. CHOW, S. HIRAYAMA and A. SUGAWARA, J. Biomed. Mater. Res. (Appl. Biomater.) 67B (2003) 689CrossRefGoogle Scholar
  16. 16.
    L. E. CAREY, H. H. K. XU, C. G. SIMON Jr, S. TAKAGI and L. C. CHOW, Biomaterials 26 (2005) 5002CrossRefGoogle Scholar
  17. 17.
    S. TAKAGI and L. C. CHOW, J. Mater. Sci.: Mater. Med. 12 (2001) 135CrossRefGoogle Scholar
  18. 18.
    H. H. K. XU, J. B. QUINN, S. TAKAGI, L. C. CHOW and F. C. EICHMILLER, J. Biomed. Mater. Res. 57 (2001) 457CrossRefGoogle Scholar
  19. 19.
    ISO 10993–5. Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity (International Standards Organization, Geneva, Switzerland, 1999)Google Scholar
  20. 20.
    C. J. DAMIEN and J. R. PARSONS, J. Appl. Biomater. 2 (1991) 187CrossRefGoogle Scholar
  21. 21.
    M. ISHIYAMA, M. SHIGA, K. SASAMOTO, M. MIZOGUCHI and P. G. HE, Chem. Pharm. Bull. 41 (1993) 1118Google Scholar
  22. 22.
    R. M. PILLIAR, M. J. FILIAGGI, J. D. WELLS, M. D. GRYNPAS and R. A. KANDEL, Biomaterials 22 (2001) 963CrossRefGoogle Scholar
  23. 23.
    K. A. HING, S. M. BEST, K. E. TANNER, W. BONFIELD and P. A. REVELL, J. Biomed. Mater. Res. 68A (2004) 187CrossRefGoogle Scholar
  24. 24.
    T. LIVINGSTON, P. DUCHEYNE and J. GARINO, J. Biomed. Mater. Res. 62 (2002) 1CrossRefGoogle Scholar
  25. 25.
    H. H. K. XU, F. C. EICHMILLER and A. A. GIUSEPPETTI, J. Biomed. Mater. Res. 52 (2000) 107CrossRefGoogle Scholar
  26. 26.
    N. TAMAI, A. MYOUI, T. TOMITA, T. NAKASE, J. TANAKA, T. OCHI and H. YOSHIKAWA, J. Biomed. Mater. Res. 59 (2002) 110CrossRefGoogle Scholar
  27. 27.
    R. WINTER, in “A consumer’s dictionary of food additives” (Crown Publishers, New York, 1978)Google Scholar
  28. 28.
    J. Darnell, H. LODISH, D. BALTIMORE, in “Molecular cell biology, 2nd edn” (Freeman and Company, New York, 1990) p. 890Google Scholar
  29. 29.
    B. ANNAZ, K. A. HING, M. KAYSER, T. BUCKLAND and L. DI SILVIOF, J. Microscopy. 215 (2004) 100CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hockin H. K. Xu
    • 1
  • Lisa E. Carey
    • 3
  • Carl G. SimonJr.
    • 2
  1. 1.American Dental Association Foundation, Paffenbarger Research CenterNational Institute of Standards and TechnologyGaithersburgUSA
  2. 2.Polymers DivisionNational Institute of Standards and TechnologyGaithersburgUSA
  3. 3.Student InternJohns Hopkins UniversityBaltimoreUSA

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