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Journal of Materials Science: Materials in Medicine

, Volume 17, Issue 9, pp 829–833 | Cite as

Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 4: Growth of rat bone marrow stromal cells in three-dimensional hydrogels with positive and negative surface charges and in polyelectrolyte complexes

  • P. Lesný
  • M. Přádný
  • P. Jendelová
  • J. Michálek
  • J. Vacík
  • E. SykováEmail author
Article

Abstract

The growth of bone marrow stromal cells was assessed in vitro in macroporous hydrogels based on 2-hydro- xyethyl methacrylate (HEMA) copolymers with different electric charges. Copolymers of HEMA with sodium methacrylate (MA) carried a negative electric charge, copolymers of HEMA with [2-(methacryloyloxy)ethyl] trimethylammonium chloride (MOETA) carried a positive electric charge and terpolymers of HEMA, MA and MOETA+ carried both, positive and negative electric charges. The charges in the polyelectrolyte complexes were shielded by counter-ions. The hydrogels had similar porosities, based on a comparison of their diffusion parameters for small cations as measured by the real-time tetramethylammonium iontophoretic method of diffusion analysis. The cell growth was studied in the peripheral and central regions of the hydrogels at 2 hours and 2, 7, 14 and 28 days after cell seeding. Image analysis revealed the highest cellular density in the HEMA-MOETA+ copolymers; most of the cells were present in the peripheral region of the hydrogels. A lower density of cells but no difference between the peripheral and central regions was observed in the HEMA-MA copolymers and in polyelectrolyte complexes. This study showed that positively charged functional groups promote the adhesion of cells.

Keywords

HEMA Bone Marrow Stromal Cell Cell Seeding Polyelectrolyte Complex Cellular Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    S. WOERLY, E. PINET, L. DE ROBERTIS, M. BOUSMINA, G. LAROCHE, T. ROITBAK, L. VARGOVA and E. SYKOVA J Biomater Sci Polym Ed 9 (1998) 681.Google Scholar
  2. 2.
    P LESNY, J. DE CROOS, M. PRADNY, J. VACIK, J. MICHALEK, S. WOERLY and E. SYKOVA J Chem Neuroanat 23 (2002) 243.CrossRefGoogle Scholar
  3. 3.
    S. WOERLY, R. MARCHAND and C. LAVALLEE Biomaterials 11 (1990) 97.CrossRefGoogle Scholar
  4. 4.
    S. WOERLY, P. PETROV, E. SYKOVA, T. ROITBAK, Z. SIMONOVA and A. R. HARVEY Tissue Eng 5 (1999) 467.Google Scholar
  5. 5.
    D. J. PROCKOP, Science 276 (1997) 71.CrossRefGoogle Scholar
  6. 6.
    M. F. PITTENGER, A. M. MACKAY, S. C. BECK, R. K. JAISWAL, R. DOUGLAS, J. D. MOSCA, M. A. MOORMAN, D. W. SIMONETTI, S. CRAIG and D. R. MARSHAK Science 284 (1999) 143.CrossRefGoogle Scholar
  7. 7.
    G. C. KOPEN, D. J. PROCKOP and D. G. PHINNEY Proc Natl Acad Sci U S A 96 (1999) 10711.CrossRefGoogle Scholar
  8. 8.
    T. R. BRAZELTON, F. M. ROSSI, G. I. KESHET and H. M. BLAU Science 290 (2000) 1775.CrossRefGoogle Scholar
  9. 9.
    P. JENDELOVA, V. HERYNEK, J. DECROOS, K. GLOGAROVA, B. ANDERSSON, M. HAJEK and E. SYKOVA Magn Reson Med 50 (2003) 767.CrossRefGoogle Scholar
  10. 10.
    X. CHEN, Y. LI, L. WANG, M. KATAKOWSKI, L. ZHANG, J. CHEN, Y. XU, S. C. GAUTAM and M. CHOPP Neuropathology 22 (2002) 275.CrossRefGoogle Scholar
  11. 11.
    N. K. LOH, S. WOERLY, S. M. BUNT, S. D. WILTON and A. R. HARVEY Exp Neurol 170 (2001) 72.CrossRefGoogle Scholar
  12. 12.
    W. J. LI, C. T. LAURENCIN, E. J. CATERSON, R. S. TUAN and F. K. KO J Biomed Mater Res 60 (2002) 613.CrossRefGoogle Scholar
  13. 13.
    D. A. STUART, C. P. PARAMORE, S. WOERLY and J. D. PEDUZZI, in Neurotrauma Society Meeting 2000.Google Scholar
  14. 14.
    A. SOEKARNO, B. LOM and P. E. HOCKBERGER Neuroimage 1 (1993) 129.CrossRefGoogle Scholar
  15. 15.
    Q. QIU, M. SAYER, M. KAWAJA, X. SHEN and J. E. DAVIES J Biomed Mater Res 42 (1998) 117.CrossRefGoogle Scholar
  16. 16.
    M. PRADNY, P. LESNY, J. FIALA, J. VACIK, M. SLOUF, J. MICHALEK and E. SYKOVA Collection of Czech Chemical Communication 68 (2003) 812.CrossRefGoogle Scholar
  17. 17.
    M. PRADNY, P. LESNY, K. SMETANA JR., J. VACIK, M. SLOUF, J. MICHALEK and E. SYKOVA J Mater Sci Mater Med 16 (2005) 767.CrossRefGoogle Scholar
  18. 18.
    C. NICHOLSON and E. SYKOVA Trends Neurosci. 21 (1998) 207.CrossRefGoogle Scholar
  19. 19.
    J. MICHALEK, M. PRADNY, A. ARTYUKHOV, M. SLOUF and K. SMETANA J Mater Sci Mater Med 16 (2005) 783.CrossRefGoogle Scholar
  20. 20.
    C. NICHOLSON and J. M. PHILLIPS J Physiol 321 (1981) 225.Google Scholar
  21. 21.
    E. SYKOVA, Neurochem Int 45 (2004) 453.CrossRefGoogle Scholar
  22. 22.
    A. LEHMENKUHLER, E. SYKOVA, J. SVOBODA, K. ZILLES and C. NICHOLSON Neuroscience 55 (1993) 339.CrossRefGoogle Scholar
  23. 23.
    E. SYKOVA, L. URDZIKOVA, P. JENDELOVA, M. BURIAN, K. GLOGAROVA and M. HAJEK Exp Neurol 193 (2005) 261.Google Scholar
  24. 24.
    P. LESNY, M. PRADNY, A. HEJCL, P. JENDELOVA, J. MICHALEK and E. SYKOVA Exp. Neurol 187 (2004) 212.Google Scholar

Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • P. Lesný
    • 1
    • 2
    • 3
  • M. Přádný
    • 3
    • 4
  • P. Jendelová
    • 1
    • 2
    • 3
  • J. Michálek
    • 3
    • 4
  • J. Vacík
    • 3
    • 4
  • E. Syková
    • 1
    • 2
    • 3
    Email author
  1. 1.Institute of Experimental MedicineAcademy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Department of NeuroscienceCharles University, Second Medical FacultyPragueCzech Republic
  3. 3.Center for Cell Therapy and Tissue RepairCharles UniversityPragueCzech Republic
  4. 4.Institute of Macromolecular ChemistryAcademy of Sciences of the Czech RepublicPragueCzech Republic

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