Journal of Materials Science: Materials in Medicine

, Volume 17, Issue 9, pp 795–802 | Cite as

Gelation under dynamic conditions: A strategy for in vitro cell ordering

  • Ernesto Doncel-Pérez
  • Margarita Darder
  • Eduardo Martín-López
  • Luis Vázquez
  • Manuel Nieto-Sampedro
  • Eduardo Ruiz-Hitzky
Article
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Accepted:

Abstract

Ordered gelation under spin-coating conditions, as reported here, is a suitable method to order cells in biogels. Cell ordering is of great importance for functional repair of central nervous system (CNS) injuries, because therapies must include strategies to bridge chystic gaps and facilitate axon growth towards its target. Organized biocompatible and biodegradable substrates may be used for this purpose, to supply trophic support and provide directional cues for neuronal process outgrowth. Atomic force microscopy (AFM) and low temperature scanning electron microscopy (LTSEM), confirmed that fibrils in κ–carrageenan/chitosan and fibrin hydrogels prepared under spin-coating conditions, were longitudinally arranged. The cell model was conveniently tested using rat C6 glioma cells. C6 cells were distributed regularly in fibrin gels formed under centrifugal force. The ability of ordered fibrin scaffolds to promote uniform distribution of transplanted cells, was confirmed by fluorescence microscopy.

Keywords

Chitosan Atomic Force Microscopy Centrifugal Force Olfactory Ensheathing Cell Atomic Force Microscopy Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Ernesto Doncel-Pérez
    • 1
  • Margarita Darder
    • 3
  • Eduardo Martín-López
    • 2
  • Luis Vázquez
    • 3
  • Manuel Nieto-Sampedro
    • 1
    • 2
  • Eduardo Ruiz-Hitzky
    • 3
  1. 1.Unidad de Neurología Experimental, CSIC, SESCAMHospital Nacional de ParapléjicosToledoSpain
  2. 2.Departamento de Plasticidad NeuralInstituto Cajal, CSICMadridSpain
  3. 3.Instituto de Ciencia de Materiales de Madrid, CSICCantoblancoMadridSpain

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