Enzymatic preparation of hollow magnetite microspheres for hyperthermic treatment of cancer

  • Masakazu Kawashita
  • Kazuo Sadaoka
  • Tadashi Kokubo
  • Takashi Saito
  • Mikio Takano
  • Norio Araki
  • Masahiro Hiraoka


Ferrimagnetic materials can be expected to be useful as thermal seeds for hyperthermic treatment of cancer, especially where the cancer is located in deep parts of body, as they can generate heat by magnetic hysteretic loss when they are placed in an alternating magnetic field. In this study, hollow magnetite (Fe3O4) particles were prepared using an enzymatic reaction of urease. A hollow particle was obtained by using a Pasteur pipette. The particle was 500 μm in size and was composed of Fe3O4. Its saturation magnetization and coercive force were 57 emu⋅g−1 and 183 Oe, respectively. Its heat generation under an alternating magnetic field of 300 Oe at 100 kHz was estimated to be 45 W⋅g−1. Microspheres 30 μm in diameter were also successfully obtained by using a spray gun.


Magnetite Alginate Saturation Magnetization Coercive Force Pasteur Pipette 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. CONWAY and A. P. ANDERSON, Clin. Phys. Physiol. Means. 7 (1986) 287.CrossRefGoogle Scholar
  2. 2.
    T. SUGIHARA, in “Gan-to-Tatakau-Hyperthermia” (Kinpoudou, Kyoto, 1986) p. 37 (In Japanese).Google Scholar
  3. 3.
  4. 4.
    K. OVERGAARD and J. OVERGAARD, Eur. J. Cancer 8 (1972) 64.Google Scholar
  5. 5.
    J. OVERGAARD, Cancer 39 (1977) 2637.Google Scholar
  6. 6.
    A. A. LUDERER, N. F. BORRELLI, J. N. PANZARINO, G. R. MANSFIELD, D. M. HESS, J. L. BROWN and E. H. BARNELL, Radiat. Res. 94 (1983) 190.Google Scholar
  7. 7.
    N. F. BORRELLI, A. A. LUDERER and J. N. PANZARINO, U. S. Pat. No. 4 323 056, 1980.Google Scholar
  8. 8.
    N. F. BORRELLI, A. A. LUDERER and J. N. PANZARINO, Phys. Med. Biol. 29 (1984) 487.CrossRefGoogle Scholar
  9. 9.
    T. KOKUBO, Y. EBISAWA, Y. SUGIMOTO, M. KIYAMA, K. OHURA, T. YAMAMURO, M. HIRAOKA and M. ABE. In “Bioceramics Vol. 3” edited by J. E. HULBERT and S. F. HULBERT (Rose-Hulman Institute of Technology, Indiana, 1990) p. 213.Google Scholar
  10. 10.
    Y. EBISAWA, Y. SUGIMOTO, T. HAYASHI, T. KOKUBO, K. OHURA and T. YAMAMURO, J. Ceram. Soc. Jpn. 99 (1991) 7.Google Scholar
  11. 11.
    Y. EBISAWA, T. KOKUBO, K. OHURA and T. YAMAMURO, J. Mater. Sci: Mater. Med. 1 (1990) 239.CrossRefGoogle Scholar
  12. 12.
    Y. EBISAWA, T. KOKUBO, K. OHURA and T. YAMAMURO, J. Mater. Sci: Mater. Med. 4 (1992) 225.CrossRefGoogle Scholar
  13. 13.
    K. OHURA, M. IKENAGA, T. NAKAMURA, T. YAMAMURO, Y. EBISAWA, T. KOKUBO, Y. KOTOURA and M. OKA, J. Appl. Biomater. 2 (1991) 153.CrossRefGoogle Scholar
  14. 14.
    M. IKENAGA, K. OHURA, T. YAMAMURO, Y. KOTOURA, M. OKA and T. KOKUBO, J. Orthop. Res. 11 (1993) 849.CrossRefGoogle Scholar
  15. 15.
    Y. EBISAWA, F. MIYAJI, T. KOKUBO, K. OHURA and T. NAKAMURA, J. Ceram. Soc. Jpn. 105 (1997) 947.Google Scholar
  16. 16.
    Y. EBISAWA, F. MIYAJI, T. KOKUBO, K. OHURA and T. NAKAMURA, Biomaterials 18 (1997) 1277.CrossRefGoogle Scholar
  17. 17.
    H. KONAKA, F. MIYAJI and T. KOKUBO, J. Ceram. Soc. Jpn. 105 (1997) 833.Google Scholar
  18. 18.
    M. KAWASHITA, H. TAKAOKA, T. KOKUBO, T. YAO, S. HAMADA and T. SHINJO, J. Ceram. Soc. Jpn. 109 (2001) 39.Google Scholar
  19. 19.
    M. KAWASHITA, Y. IWAHASHI, T. KOKUBO, T. YAO, S. HAMADA and T. SHINJO, J. Ceram. Soc. Jpn. 112 (2004) 373.CrossRefGoogle Scholar
  20. 20.
    M. KAWASHITA, M. TANAKA, T. KOKUBO, Y. INOUE, T. YAO, S. HAMADA and T. SHINJO, Biomaterials 26 (2005) 2231.CrossRefGoogle Scholar
  21. 21.
    Y. HIROSE, M. EBINA, K. WATANABE, H. UNUMA and M. SUGAWARA. In Proceedings of 15th Fall Meeting of the Ceramic Society of Japan, Akita, September 2002, edited by the Ceramic Society of Japan (the Ceramic Society of Japan, Tokyo, 2002) p. 109 (in Japanese).Google Scholar
  22. 22.
    H. UNUMA, Y. HIROSE, M. ITO and K. WATANABE, J. Ceram. Soc. Jpn. 112 (2004) 409.CrossRefGoogle Scholar
  23. 23.
    A. Y. MATLOUBIEH, R. B. ROEMER and T. C. CETAS, IEEE Trans. Biomed. Eng. 31 (1984) 227.Google Scholar
  24. 24.
    A. MUAN, Am. Ceram. Soc. Bull. 37 (1958) 81.Google Scholar
  25. 25.
    J. S. JACOB and C. P. BEAN. In “Magnetism III” edited by G. T. RADO and H. SUHL (Academic Press Inc., New York, 1963) p. 371.Google Scholar
  26. 26.
    J. E. KNOWLES, J. Magn., Magn. Mater. 25 (1981) 105.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Masakazu Kawashita
    • 1
  • Kazuo Sadaoka
    • 1
  • Tadashi Kokubo
    • 2
  • Takashi Saito
    • 3
  • Mikio Takano
    • 3
  • Norio Araki
    • 4
  • Masahiro Hiraoka
    • 4
  1. 1.Graduate School of EngineeringKyoto UniversityKyotoJapan
  2. 2.Institute of Science and Technology ResearchChubu UniversityAichiJapan
  3. 3.Institute for Chemical ResearchKyoto UniversityKyotoJapan
  4. 4.Graduate School of MedicineKyoto UniversityKyotoJapan

Personalised recommendations